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APC anti-human CD127 (IL-7Rα) Antibody

Pricing & Availability
Clone
A019D5 (See other available formats)
Regulatory Status
RUO
Other Names
IL-7 receptor α chain, IL-7Rα
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A019D5_APC_CD127_Antibody_FC_1_102512
Human peripheral blood lymphocytes were stained with CD3 FITC and CD127 (clone A019D5) APC (top) or mouse IgG1 APC isotype control (bottom).
  • A019D5_APC_CD127_Antibody_FC_1_102512
    Human peripheral blood lymphocytes were stained with CD3 FITC and CD127 (clone A019D5) APC (top) or mouse IgG1 APC isotype control (bottom).
  • A019D5_APC_CD127_Antibody_FC_2_102512
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Cat # Size Price Quantity Check Availability Save
351315 25 tests $142
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351342 100 µg $296
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351316 100 tests $314
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Description

CD127 is a 60-90 kD type I transmembrane glycoprotein also known as IL-7 receptor α chain or IL-7Rα. It forms a heterodimer with the common γ chain (γc or CD132) which is shared with the receptors for IL-2, IL-4, IL-9, IL-13, IL-15, and IL-21. CD127 is expressed on immature B cells through early pre-B stage cells, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, and bone marrow stromal cells. CD127 has been reported to be a useful marker for identifying memory and effector T cells. Studies have shown that CD127 expression is down-modulated on Treg cells. It can be used as a marker for differentiation of Treg and conventional T cells. The ligation of IL-7 with its receptor is important for stimulation of mature and immature T cells as well as immature B cell proliferation and development.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human CD127
Formulation
µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
µg sizes: 0.2 mg/mL
test sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported (for the relevant formats) application: proteogenomics1.

Application References

(PubMed link indicates BioLegend citation)
  1. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations
  1. Sudmeier LJ, et al. 2022. Cell Rep Med. 3:100620. PubMed
  2. Cui B, et al. 2023. Int Immunopharmacol. 114:109541. PubMed
  3. Ye L, et al. 2022. Cell Metab. 34:595. PubMed
  4. Avital G, et al. 2022. Cell Rep. 41:111477. PubMed
  5. Rendeiro AF, et al. 2020. Nat Commun. 11:577. PubMed
  6. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  7. Vandooren J, et al. 2021. Front Immunol. 12:701739. PubMed
  8. Hixon JA, et al. 2020. Leukemia. 34:35. PubMed
  9. Alissafi T, et al. 2020. Cell Metabolism. 32(4):591-604.e7. PubMed
  10. Meyer LK, et al. 2019. J Clin Invest. 130:863. PubMed
  11. Christophersen A, et al. 2016. J Immunol. 196: 2819 - 2826. PubMed
  12. Kim SY, et al. 2018. Invest Ophthalmol Vis Sci. 59:5693. PubMed
  13. Wang B, et al. 2017. Stem Cell Res Ther.. 10.1186/s13287-017-0744-6. PubMed
  14. Grivas A, et al. 2022. Front Immunol. 13:964274. PubMed
  15. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  16. Goods BA, et al. 2021. JCI Insight. 6: . PubMed
  17. Shen C, et al. 2021. Front Immunol. 12:680055. PubMed
  18. Batikan O, et al. 2022. J Coll Physicians Surg Pak. 32:1004. PubMed
  19. Chruewkamlow N, et al. 2021. Stem Cell Res Ther. 12:520. PubMed
  20. Schielke L, et al. 2022. Front Immunol. 13:916701. PubMed
  21. Nabatanzi R, et al. 2021. BMC Immunol. 22:59. PubMed
  22. Grigoriou M, et al. 2021. Cancer Immunol Res. 9:726. PubMed
  23. Imura Y, et al. 2020. JCI Insight. :5. PubMed
  24. Janssen JJE, et al. 2022. Am J Physiol Endocrinol Metab. 322:E141. PubMed
  25. Sehgal R, et al. 2022. Front Immunol. 13:828949. PubMed
  26. Li J, et al. 2017. Toxicology. 378:17. PubMed
  27. Wang L, et al. 2018. Oncol Lett. 15:8635. PubMed
  28. Gamradt S, et al. 2021. iScience. 24:103312. PubMed
  29. Rochman Y, et al. 2015. PLoS One. 10:122198. PubMed
RRID
AB_2564137 (BioLegend Cat. No. 351315)
AB_2564137 (BioLegend Cat. No. 351342)
AB_2564137 (BioLegend Cat. No. 351316)

Antigen Details

Structure
Type I transmembrane glycoprotein, associates with CD132, 60-90 kD
Distribution

Immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, bone marrow stromal cells

Function
T cell and immature B cell proliferation and development
Ligand/Receptor
IL-7
Cell Type
B cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Sudo T, et al. 1993. P. Natl. Acad. Sci. USA 90:9125.
2. He YW and Malek TR. 1998. Crit. Rev. Immunol. 18:503.
3. Huster KM, et al. 2004. P. Natl. Acad. Sci. USA 101:5610.
4. Pillai M, et al. 2004. Leukemia Lymphoma 45:2403.
5. Morrissey PJ, et al. 1989. J. Exp. Med. 169:707.
6. Liu W, et al. 2006. J. Exp. Med. 203:1701.

Gene ID
3575 View all products for this Gene ID
UniProt
View information about CD127 on UniProt.org

Other Formats

View All CD127 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-human CD127 (IL-7Rα) A019D5 FC
PE anti-human CD127 (IL-7Rα) A019D5 FC
Pacific Blue™ anti-human CD127 (IL-7Rα) A019D5 FC
Brilliant Violet 421™ anti-human CD127 (IL-7Rα) A019D5 FC
FITC anti-human CD127 (IL-7Rα) A019D5 FC
Alexa Fluor® 488 anti-human CD127 (IL-7Rα) A019D5 FC
APC anti-human CD127 (IL-7Rα) A019D5 FC
Alexa Fluor® 647 anti-human CD127 (IL-7Rα) A019D5 FC
PE/Cyanine7 anti-human CD127 (IL-7Rα) A019D5 FC
PerCP/Cyanine5.5 anti-human CD127 (IL-7Rα) A019D5 FC
Brilliant Violet 570™ anti-human CD127 (IL-7Rα) A019D5 FC
PE/Cyanine5 anti-human CD127 (IL-7Rα) A019D5 FC
Brilliant Violet 650™ anti-human CD127 (IL-7Rα) A019D5 FC
Brilliant Violet 711™ anti-human CD127 (IL-7Rα) A019D5 FC
Brilliant Violet 785™ anti-human CD127 (IL-7Rα) A019D5 FC
Brilliant Violet 510™ anti-human CD127 (IL-7Rα) A019D5 FC
Brilliant Violet 605™ anti-human CD127 (IL-7Rα) A019D5 FC
PE/Dazzle™ 594 anti-human CD127 (IL-7Rα) A019D5 FC
Purified anti-human CD127 (IL-7Rα) (Maxpar® Ready) A019D5 FC,CyTOF®
Alexa Fluor® 700 anti-human CD127 (IL-7Rα) A019D5 FC
Biotin anti-human CD127 (IL-7Rα) A019D5 FC
APC/Cyanine7 anti-human CD127 (IL-7Rα) A019D5 FC
APC/Fire™ 750 anti-human CD127 (IL-7Rα) A019D5 FC
TotalSeq™-A0390 anti-human CD127 (IL-7Rα) A019D5 PG
TotalSeq™-B0390 anti-human CD127 (IL-7Rα) A019D5 PG
TotalSeq™-C0390 anti-human CD127 (IL-7Rα) A019D5 PG
KIRAVIA Blue 520™ anti-human CD127 (IL-7Rα) A019D5 FC
Spark NIR™ 685 anti-human CD127 (IL-7Rα) A019D5 FC
PE/Fire™ 640 anti-human CD127 (IL-7Rα) A019D5 FC
PE/Fire™ 700 anti-human CD127 (IL-7Rα) Antibody A019D5 FC
Spark YG™ 581 anti-human CD127 (IL-7Rα) A019D5 FC
Brilliant Violet 750™ anti-human CD127 (IL-7Rα) A019D5 FC
TotalSeq™-D0390 anti-human CD127 (IL-7Rα) A019D5 PG
APC/Fire™ 810 anti-human CD127 (IL-7Rα) Antibody A019D5 FC
APC/Fire™ 750 anti-human CD127 A019D5 FC
PE anti-human CD127 A019D5 FC
PerCP/Cyanine5.5 anti-human CD127 A019D5 FC
PE/Cyanine7 anti-human CD127 A019D5 FC
Spark Red™ 718 anti-human CD127 (IL-7Rα) A019D5 FC
GMP PE/Cyanine7 anti-human CD127 (IL-7Rα) A019D5 FC
PE/Fire™ 744 anti-human CD127 (IL-7Rα) A019D5 FC
PerCP/Fire™ 780 anti-human CD127 (IL-7Rα) A019D5 FC
PerCP/Fire™ 806 anti-human CD127 (IL-7Rα) A019D5 FC
Spark PLUS B550™ anti-human CD127 (IL-7Rα) A019D5 FC
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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