GMP PE/Cyanine7 anti-human CD127 (IL-7Rα) Antibody, CD127, A019D5

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Clone: A019D5 (See other available formats)
Other Names: IL-7 receptor α chain, IL-7Rα
Isotype: Mouse IgG1, κ
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Product Citations: publications

A019D5_GMP_PE-Cyanine7_CD127_08102023 — Typical results from human peripheral blood lymphocytes stained either with A019D5 PE/Cyanine7 used at 5 µL/test (filled histogram) or with an isotype control (open histogram).

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260286

100 tests

$440

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Description

CD127 is a 60-90 kD type I transmembrane glycoprotein also known as IL-7 receptor α chain or IL-7Rα. It forms a heterodimer with the common γ chain (γc or CD132) which is shared with the receptors for IL-2, IL-4, IL-9, IL-13, IL-15, and IL-21. CD127 is expressed on immature B cells through early pre-B stage cells, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, and bone marrow stromal cells. CD127 has been reported to be a useful marker for identifying memory and effector T cells. Studies have shown that CD127 expression is down-modulated on Treg cells. It can be used as a marker for differentiation of Treg and conventional T cells. The ligation of IL-7 with its receptor is important for stimulation of mature and immature T cells as well as immature B cell proliferation and development.

Product Details

Technical data sheet

Product Details

Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human CD127
Formulation: Phosphate-buffered solution, pH 7.2, containing True-Stain Monocyte Blocker™, 0.09% sodium azide and 0.2% (w/v) BSA (origin USA) and a stabilizer.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: 100.0 µg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported (for the relevant formats) application: proteogenomics¹.

Antigen Details

Structure: Type I transmembrane glycoprotein, associates with CD132, 60-90 kD
Distribution: Immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, bone marrow stromal cells
Function: T cell and immature B cell proliferation and development
Ligand/Receptor: IL-7
Cell Type: B cells, T cells, Thymocytes, Tregs
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Sudo T, et al. 1993. P. Natl. Acad. Sci. USA 90:9125.
2. He YW and Malek TR. 1998. Crit. Rev. Immunol. 18:503.
3. Huster KM, et al. 2004. P. Natl. Acad. Sci. USA 101:5610.
4. Pillai M, et al. 2004. Leukemia Lymphoma 45:2403.
5. Morrissey PJ, et al. 1989. J. Exp. Med. 169:707.
6. Liu W, et al. 2006. J. Exp. Med. 203:1701.
Gene ID: 3575 View all products for this Gene ID
UniProt: View information about CD127 on UniProt.org

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Other Formats

View All CD127 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human CD127 (IL-7Rα)	A019D5	FC
PE anti-human CD127 (IL-7Rα)	A019D5	FC
Pacific Blue™ anti-human CD127 (IL-7Rα)	A019D5	FC
Brilliant Violet 421™ anti-human CD127 (IL-7Rα)	A019D5	FC
FITC anti-human CD127 (IL-7Rα)	A019D5	FC
Alexa Fluor® 488 anti-human CD127 (IL-7Rα)	A019D5	FC
APC anti-human CD127 (IL-7Rα)	A019D5	FC
Alexa Fluor® 647 anti-human CD127 (IL-7Rα)	A019D5	FC
PE/Cyanine7 anti-human CD127 (IL-7Rα)	A019D5	FC
PerCP/Cyanine5.5 anti-human CD127 (IL-7Rα)	A019D5	FC
Brilliant Violet 570™ anti-human CD127 (IL-7Rα)	A019D5	FC
PE/Cyanine5 anti-human CD127 (IL-7Rα)	A019D5	FC
Brilliant Violet 650™ anti-human CD127 (IL-7Rα)	A019D5	FC
Brilliant Violet 711™ anti-human CD127 (IL-7Rα)	A019D5	FC
Brilliant Violet 785™ anti-human CD127 (IL-7Rα)	A019D5	FC
Brilliant Violet 510™ anti-human CD127 (IL-7Rα)	A019D5	FC
Brilliant Violet 605™ anti-human CD127 (IL-7Rα)	A019D5	FC
PE/Dazzle™ 594 anti-human CD127 (IL-7Rα)	A019D5	FC
Purified anti-human CD127 (IL-7Rα) (Maxpar® Ready)	A019D5	FC,CyTOF®
Alexa Fluor® 700 anti-human CD127 (IL-7Rα)	A019D5	FC
Biotin anti-human CD127 (IL-7Rα)	A019D5	FC
APC/Cyanine7 anti-human CD127 (IL-7Rα)	A019D5	FC
APC/Fire™ 750 anti-human CD127 (IL-7Rα)	A019D5	FC
TotalSeq™-A0390 anti-human CD127 (IL-7Rα)	A019D5	PG
TotalSeq™-B0390 anti-human CD127 (IL-7Rα)	A019D5	PG
TotalSeq™-C0390 anti-human CD127 (IL-7Rα)	A019D5	PG
KIRAVIA Blue 520™ anti-human CD127 (IL-7Rα)	A019D5	FC
Spark NIR™ 685 anti-human CD127 (IL-7Rα)	A019D5	FC
PE/Fire™ 640 anti-human CD127 (IL-7Rα)	A019D5	FC
PE/Fire™ 700 anti-human CD127 (IL-7Rα) Antibody	A019D5	FC
Spark YG™ 581 anti-human CD127 (IL-7Rα)	A019D5	FC
Brilliant Violet 750™ anti-human CD127 (IL-7Rα)	A019D5	FC
TotalSeq™-D0390 anti-human CD127 (IL-7Rα)	A019D5	PG
APC/Fire™ 810 anti-human CD127 (IL-7Rα) Antibody	A019D5	FC
APC/Fire™ 750 anti-human CD127	A019D5	FC
PE anti-human CD127	A019D5	FC
PerCP/Cyanine5.5 anti-human CD127	A019D5	FC
PE/Cyanine7 anti-human CD127	A019D5	FC
Spark Red™ 718 anti-human CD127 (IL-7Rα)	A019D5	FC
GMP PE/Cyanine7 anti-human CD127 (IL-7Rα)	A019D5	FC
PE/Fire™ 744 anti-human CD127 (IL-7Rα)	A019D5	FC
PerCP/Fire™ 780 anti-human CD127 (IL-7Rα)	A019D5	FC
PerCP/Fire™ 806 anti-human CD127 (IL-7Rα)	A019D5	FC
Spark PLUS B550™ anti-human CD127 (IL-7Rα)	A019D5	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 09/07/2023

For Research Use Only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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