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APC anti-human CD3 Antibody

Pricing & Availability
Clone
HIT3a (See other available formats)
Regulatory Status
RUO
Workshop
V CD03.05
Other Names
T3, CD3ε
Isotype
Mouse IgG2a, κ
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Product Citations
publications
HIT3a_APC_073004
Human peripheral blood lymphocytes stained with HIT3a APC
  • HIT3a_APC_073004
    Human peripheral blood lymphocytes stained with HIT3a APC
Compare all formats See APC spectral data
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300311 25 tests $33
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300312 100 tests $83
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Description

CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T lymphocytes, NK-T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported (for the relevant formats) applications include: immunohistochemical staining of acetone-fixed frozen sections, immunoprecipitation, and activation of T lymphocytes4-7. The HIT3a antibody is able to stimulate T cell activation. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300314). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 300332) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York.
  3. Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego.
  4. Sedelies KA, et al. 2004. J. Biol. Chem. 279:26581. (Activ)
  5. Rivollier A, et al. 2004. Blood 104:4029. (Activ)
  6. Scharschmidt E, et al. 2004. Mol. Cell Biol. 24:3860. (Activ)
  7. Smeltz RB. 2007. J. Immunol. 178:4786. (Activ)
Product Citations
  1. Dong MB, et al. 2020. Cell. 178(5):1189-1204.e23.. PubMed
  2. Pan J, et al. 2021. J Cell Mol Med. 25:1089. PubMed
  3. Liu C, et al. 2022. Front Immunol. 13:1039631. PubMed
  4. Gerace D, et al. 2023. Cell Rep Med. 4:100879. PubMed
  5. Tunali G, et al. 2023. J Clin Invest. :. PubMed
  6. Ye L, et al. 2022. Cell Metab. 34:595. PubMed
  7. Tate T, et al. 2023. Cancers (Basel). 15:. PubMed
  8. Yuan X, et al. 2023. Oncoimmunology. 12:2219544. PubMed
  9. Zeng W, et al. 2017. Front Immunol. 0.806944444. PubMed
  10. Lopez-Vergès S, et al. 2010. Blood. 116:3865. PubMed
  11. Morton JJ, et al. 2018. Mol Carcinog. 57:1651. PubMed
  12. Ge Y, et al. 2019. J Immunol. 203:2827. PubMed
  13. Huang C, et al. 2020. Theranostics. 10:10498. PubMed
  14. Yomogida K, et al. 2012. Cytokine. 58:431. PubMed
  15. Merugu S, et al. 2020. Methods Enzymol. 639:289. PubMed
  16. Chulpanova DS, et al. 2020. Bioengineering (Basel). 7:00. PubMed
  17. Alsaleh G, et al. 2020. Elife. 9: . PubMed
  18. Domenis R, et al. 2017. PLoS One. 10.1371/journal.pone.0169932. PubMed
  19. Cao Y, et al. 2021. Cell Res. 31:732. PubMed
  20. Mao FY, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:395. PubMed
  21. Zhang J, et al. 2022. Nature. 609:369. PubMed
  22. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  23. Jung JH, et al. 2021. Nat Commun. 12:4043. PubMed
  24. Morton JJ, et al. 2020. Molecular Cancer Research. 19(2):346-357. PubMed
  25. Roider T, et al. 2021. Blood Adv. 5:5060. PubMed
  26. Wang L, et al. 2021. Microbiol Spectr. 9:e0124621. PubMed
  27. Ren X, et al. 2022. STAR Protoc. 3:101818. PubMed
  28. Mlynska A, et al. 2020. Am J Reprod Immunol. 84:e13244. PubMed
  29. Franco LM, et al. 2019. J Exp Med. 216:384. PubMed
  30. Yin S, et al. 2015. Sci Rep. 5: 14432. PubMed
  31. Gomzikova MO, et al. 2020. Pharmaceutics. 12:00. PubMed
  32. Hu Q, et al. 2021. Nat Commun. 12:2186. PubMed
  33. Lee YG, et al. 2019. Nat Commun. 10:2681. PubMed
  34. Liu X, et al. 2020. Ther Adv Med Oncol. 12:1758835920910347. PubMed
  35. Reyes M, et al. 2021. Sci Transl Med. 13:. PubMed
  36. Hu Q, et al. 2021. Nat Biomed Eng. 5:1038. PubMed
  37. Hu JF, et al. 2022. Front Immunol. 13:958960. PubMed
  38. Li X, et al. 2021. Front Oncol. 11:703087. PubMed
  39. Ruth JH, et al. 2021. JCI Insight. 6:e145662. PubMed
  40. Hara T, et al. 2021. Cancer Cell. 39(6):779-792.e11. PubMed
  41. Tao L, et al. 2020. Cancers (Basel). 12:00. PubMed
  42. Jiang L, et al. 2019. Sci Rep. 9:3705. PubMed
  43. Wang Z, et al. 2018. Front Immunol. 9:1239. PubMed
  44. Zhong W, et al. 2022. Front Immunol. 13:1001255. PubMed
  45. Mlynska A, et al. 2019. Oncol Rep. 41:1238. PubMed
RRID
AB_314047 (BioLegend Cat. No. 300311)
AB_314047 (BioLegend Cat. No. 300312)

Antigen Details

Structure
Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution

Mature T and NK-T cells, thymocyte differentiation

Function
Antigen recognition, signal transduction, T cell activation
Ligand/Receptor
Peptide antigen bound to MHC
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, TCRs
Antigen References

1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507.

Gene ID
916 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

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Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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