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Purified anti-human CD3 Antibody

Pricing & Availability
Clone
HIT3a (See other available formats)
Regulatory Status
RUO
Workshop
V CD03.05
Other Names
T3, CD3ε
Isotype
Mouse IgG2a, κ
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Product Citations
publications
HIT3a_Purified_080204
Human peripheral blood lymphocytes stained with purified HIT3a and detected with anti-mouse IgGs FITC
  • HIT3a_Purified_080204
    Human peripheral blood lymphocytes stained with purified HIT3a and detected with anti-mouse IgGs FITC
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300301 25 µg $24
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300302 100 µg $50
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Description

CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T lymphocytes, NK-T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IP, IHC-F, Activ - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported (for the relevant formats) applications include: immunohistochemical staining of acetone-fixed frozen sections, immunoprecipitation, and activation of T lymphocytes4-7. The HIT3a antibody is able to stimulate T cell activation. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300314). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 300332) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York.
  3. Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego.
  4. Sedelies KA, et al. 2004. J. Biol. Chem. 279:26581. (Activ)
  5. Rivollier A, et al. 2004. Blood 104:4029. (Activ)
  6. Scharschmidt E, et al. 2004. Mol. Cell Biol. 24:3860. (Activ)
  7. Smeltz RB. 2007. J. Immunol. 178:4786. (Activ)
Product Citations
  1. Lu X, et al. 2021. J Exp Med. 218: . PubMed
  2. Toothaker JM, et al. 2022. Development. 149: . PubMed
  3. Lin GL, et al. 2018. Acta Neuropathol Commun. 9:617. PubMed
  4. Kaushal A, et al. 2021. Blood Cancer Discov. 2:600. PubMed
  5. Galaski J, et al. 2021. Front Cell Infect Microbiol. 11:692544. PubMed
  6. Yang W, et al. 2020. Nat Commun. 3.553472222. PubMed
  7. Kim H, et al. 2021. Int J Mol Sci. 22:. PubMed
  8. Gao H, et al. 2021. J Virol. :. PubMed
  9. Patel AA, et al. 2017. J Exp Med. 214:1913. PubMed
  10. Willimsky G, et al. 2021. Elife. 10:. PubMed
  11. Chakhtoura M, et al. 2021. PLoS Pathog. 17:e1009732. PubMed
  12. Vereertbrugghen A, et al. 2021. Front Oncol. 11:598319. PubMed
  13. Zhang Y, et al. 2022. Exp Hematol Oncol. 11:15. PubMed
  14. Kondo H, et al. 2022. Front Immunol. 13:836923. PubMed
  15. Liu F, et al. 2020. J Immunother Cancer. 8:. PubMed
  16. Kilpinen L, et al. 2013. J Lipid Res. 54:622. PubMed
  17. Jin H, et al. 2019. MBio. 10:e01769-19. PubMed
  18. Cobb DA, et al. 2022. J Immunother Cancer. 10:. PubMed
  19. Cosgrove PR, et al. 2021. Pediatr Res. . PubMed
  20. Cai J, et al. 2021. eLife. 10:00. PubMed
  21. Bruno TC, et al. 2017. Cancer Immunol Res. 0.831944444. PubMed
  22. Liu Y, et al. 2022. Micromachines (Basel). 13:. PubMed
  23. Zhao J, et al. 2017. Sci Immunol. 2:eaan5400. PubMed
  24. Bonavia AS, et al. 2022. Front Immunol. 13:940030. PubMed
  25. Newell KL, et al. 2021. PLoS One. 16:e0244855. PubMed
  26. Vlkova M, et al. 2019. J Immunol. 202:93. PubMed
  27. De Maeyer RPH, et al. 2020. Nat Immunol. 21:615. PubMed
  28. Sheng WY, et al. 2006. FEBS Lett. 580:6819. PubMed
  29. Mazer M, et al. 2019. J Immunol. 203:2088. PubMed
  30. Jones N, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01516. PubMed
  31. Bantug GR, et al. 2018. Immunity. 48:542. PubMed
  32. Ren X, et al. 2021. Lab Chip. 21:1527. PubMed
  33. Ravell JC, et al. 2020. J Clin Invest. 130:507. PubMed
  34. Ainai A, et al. 2020. Microbiol Immunol. 64:313. PubMed
RRID
AB_314037 (BioLegend Cat. No. 300301)
AB_314037 (BioLegend Cat. No. 300302)

Antigen Details

Structure
Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution

Mature T and NK-T cells, thymocyte differentiation

Function
Antigen recognition, signal transduction, T cell activation
Ligand/Receptor
Peptide antigen bound to MHC
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, TCRs
Antigen References

1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507.

Gene ID
916 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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