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APC anti-human Siglec-10 Antibody

Pricing & Availability
Clone
5G6 (See other available formats)
Regulatory Status
RUO
Other Names
Sialic acid binding Ig-like lectin 10
Isotype
Mouse IgG1, κ
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Product Citations
publications
5G6_APC_1_033012
Human peripheral blood lymphocytes were stained with CD19 FITC and Siglec-10 (clone 5G6) APC (top) or mouse IgG1, κ APC isotype control (bottom).
  • 5G6_APC_1_033012
    Human peripheral blood lymphocytes were stained with CD19 FITC and Siglec-10 (clone 5G6) APC (top) or mouse IgG1, κ APC isotype control (bottom).
  • 5G6_APC_2_033012
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347606 100 tests $367
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Description

Siglec-10 is a lectin that specifically binds α-2,3- or α-2,6-linked sialic acid. It is a member of the Ig-superfamily and is expressed in monocytes, eosinophils, B cells, and a subset of NK cells. Siglec-10 is a single pass transmembrane protein. The extracellular domain contains one V-set Ig-like domain and three C2-set domains; the cytoplasmic domain contains one immunoreceptor tyrosine-based inhibitor motif (ITIM) which, after tyrosine-phosphorylation, recruits SH2-family phosphatases such as SHP-1 and PTPN6, resulting in the blocking of the signal transduction. CD24 and the vascular adhesion protein-1 (VAP-1) have been described as ligands of Siglec-10.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant Siglec-10 fused to human IgG Fc
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Munday J, et al. 2001. Biochem. J. 355:489
  2. Yokoi H, et al. 2006. Allergy. 61:769
  3. Kivi E, et al. 2009. Blood 114:5385
  4. Barkal AA, et al. 2019. Nature. 572(7769):392-396. (Agonist)
Product Citations
  1. Murugesan G, et al. 2022. Biomedicines. 10:. PubMed
  2. Keumatio Doungtsop BC, et al. 2024. J Allergy Clin Immunol Glob. 100193:3. PubMed
  3. Tecle E, et al. 2019. J Biol Chem. 294:11910. PubMed
RRID
AB_11203899 (BioLegend Cat. No. 347606)

Antigen Details

Structure
Lectin, belongs to the Ig-superfamily, has one V-set Ig-like domain and three C2-set domains, a single transmembrane domain and one immunoreceptor tyrosine-based inhibitor motif (ITIM) in the cytoplasmic domain
Distribution

Monocytes, eosinophils, B cells, and a subset of NK cells

Function
Cell adhesion, signal transduction
Interaction
SHP-1, PTPN6
Ligand/Receptor
CD24, vascular adhesion protein-1 (VAP-1)
Bioactivity
Inhibition of signal transduction by recruiting cytoplasmic phosphatases
Cell Type
B cells, Eosinophils, Monocytes, NK cells
Biology Area
Immunology
Molecular Family
Siglec Molecules
Antigen References

1. Chen GY, et al. 2009. Science 323:1722
2. Aizawa H, et al. 2003. Genomics.82:521
3. Kitzig F, et al. 2002. Biochem Biophys Res Commun. 296:355

Gene ID
89790 View all products for this Gene ID
UniProt
View information about Siglec-10 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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