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Brilliant Violet 421™ anti-human Siglec-10 Antibody

Pricing & Availability
Clone
5G6 (See other available formats)
Regulatory Status
RUO
Other Names
Sialic acid binding Ig-like lectin 10
Isotype
Mouse IgG1, κ
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Product Citations
publications
5G6_BV421_Siglec_10_Antibody_070623
Human peripheral blood lymphocytes were stained with anti-human Siglec-10 (clone 5G6) Brilliant Violet 421™ (left) or mouse IgG1, к Brilliant Violet 421™ isotype control (right). Cells were costained with anti-human CD19 FITC.
  • 5G6_BV421_Siglec_10_Antibody_070623
    Human peripheral blood lymphocytes were stained with anti-human Siglec-10 (clone 5G6) Brilliant Violet 421™ (left) or mouse IgG1, к Brilliant Violet 421™ isotype control (right). Cells were costained with anti-human CD19 FITC.
Compare all formats See Brilliant Violet 421™ spectral data
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347619 25 tests $205
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347620 100 tests $420
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Description

Siglec-10 is a lectin that specifically binds α-2,3- or α-2,6-linked sialic acid. It is a member of the Ig-superfamily and is expressed in monocytes, eosinophils, B cells, and a subset of NK cells. Siglec-10 is a single pass transmembrane protein. The extracellular domain contains one V-set Ig-like domain and three C2-set domains; the cytoplasmic domain contains one immunoreceptor tyrosine-based inhibitor motif (ITIM) which, after tyrosine-phosphorylation, recruits SH2-family phosphatases such as SHP-1 and PTPN6, resulting in the blocking of the signal transduction. CD24 and the vascular adhesion protein-1 (VAP-1) have been described as ligands of Siglec-10.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant Siglec-10 fused to human IgG Fc
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Munday J, et al. 2001. Biochem. J. 355:489
  2. Yokoi H, et al. 2006. Allergy. 61:769
  3. Kivi E, et al. 2009. Blood 114:5385
  4. Barkal AA, et al. 2019. Nature. 572(7769):392-396. (Agonist)
RRID
AB_3083335 (BioLegend Cat. No. 347619)
AB_3083335 (BioLegend Cat. No. 347620)

Antigen Details

Structure
Lectin, belongs to the Ig-superfamily, has one V-set Ig-like domain and three C2-set domains, a single transmembrane domain and one immunoreceptor tyrosine-based inhibitor motif (ITIM) in the cytoplasmic domain
Distribution

Monocytes, eosinophils, B cells, and a subset of NK cells

Function
Cell adhesion, signal transduction
Interaction
SHP-1, PTPN6
Ligand/Receptor
CD24, vascular adhesion protein-1 (VAP-1)
Bioactivity
Inhibition of signal transduction by recruiting cytoplasmic phosphatases
Cell Type
B cells, Eosinophils, Monocytes, NK cells
Biology Area
Immunology
Molecular Family
Siglec Molecules
Antigen References

1. Chen GY, et al. 2009. Science 323:1722
2. Aizawa H, et al. 2003. Genomics.82:521
3. Kitzig F, et al. 2002. Biochem Biophys Res Commun. 296:355

Gene ID
89790 View all products for this Gene ID
UniProt
View information about Siglec-10 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 1    Revision Date: 07/06/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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