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APC anti-mouse CD19 Antibody

Pricing & Availability
Clone
6D5 (See other available formats)
Regulatory Status
RUO
Other Names
B4
Isotype
Rat IgG2a, κ
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Product Citations
publications
6D5_APC_080207
C57BL/6 mouse splenocytes stained with CD19 (clone 6D5) APC (filled histogram) or rat IgG2a, κ APC isotype control (open histogram).
  • 6D5_APC_080207
    C57BL/6 mouse splenocytes stained with CD19 (clone 6D5) APC (filled histogram) or rat IgG2a, κ APC isotype control (open histogram).
Compare all formats See APC spectral data
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115511 25 µg $43
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115512 100 µg $128
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Description

CD19 is a 95 kD glycoprotein also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81, forms a molecular complex integral to B cell activation.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CD19-expressing K562 human erythroleukemia cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence7.

Application References

(PubMed link indicates BioLegend citation)
  1. Shoham T, et al. 2003. J. Immunol. 171:4062. (FC)
  2. Goodyear CS, et al. 2004. J. Immunol. 172:2870. (FC)
  3. Kamimura D, et al. 2006. J. Immunol. 177:306. (FC)
  4. Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (FC)
  5. Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC)
  6. Phan TG, et al. 2007. Nat. Immunol. 8:992. (FC)
  7. Hayashida K, et al. 2008. J. Biol. Chem. 283:19895. (IF) PubMed
  8. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  9. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  10. Stadnisky MD, et al. 2011. Blood. 117:5133. (FC) PubMed
  11. Perlot T, et al. 2012. J. Immunol. 188:1201. (FC) PubMed
  12. Olive V, et al. 2013. Elife. 2:822. PubMed
  13. Miyai T, et al. 2014. PNAS. 111:11780. PubMed
Product Citations
  1. Valladao A, et al. 2016. J Immunol. 197(12):4541-4551. PubMed
  2. Hou X, et al. 2020. Cell Reports. 28(1):172-189.e7.. PubMed
  3. Fu A, et al. 2022. Cell. 185:1356. PubMed
  4. Yang K, et al. 2022. Sci Immunol. 7:eabn2888. PubMed
  5. Urata S, et al. 2023. Microbiol Immunol. 67:105. PubMed
  6. Oh S, et al. 2023. Nat Biotechnol. :. PubMed
  7. Tan Y, et al. 2023. NPJ Regen Med. 8:7. PubMed
  8. Wei X, et al. 2022. Cell Rep. 40:111313. PubMed
  9. Cheng C, et al. 2022. Cell Mol Gastroenterol Hepatol. 15:261. PubMed
  10. Weier A, et al. 2022. Int J Mol Sci. 23:. PubMed
  11. Lentucci C, et al. 2017. J Biol Chem. 292:2754-2772. PubMed
  12. Schmidt M, et al. 2012. J Virol. 86:11654. PubMed
  13. Poh AR, et al. 2017. Cancer Cell. 31:563. PubMed
  14. Miyai T, et al. 2014. Proc Natl Acad Sci U S A. 111:1780. PubMed
  15. Wang J, et al. 2021. ACS Nano. 15:14360. PubMed
  16. Fu Y, et al. 2020. Mol Ther. 1214:28. PubMed
  17. Larsen SE, et al. 2021. Sci Rep. 11:9040. PubMed
  18. Brooks JF, et al. 2020. Cytotherapy. 22:436. PubMed
  19. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  20. Swidergall M, et al. 2019. Cell Rep. 28:423. PubMed
  21. Olive V, et al. 2009. Genes Dev. 2.929861111. PubMed
  22. Hoffman D, et al. 2021. Immunity. 54:2712. PubMed
  23. Lu Y, et al. 2020. Cell. 180(6):1081-1097. PubMed
  24. Sarapulov AV, et al. 2020. Front Immunol. 11:599. PubMed
  25. Delgado‐Benito V et al. 2018. Molecular cell. 72(4):636-649 . PubMed
  26. Best SA, et al. 2018. Cell Metab. 27:935. PubMed
  27. Mauduit O, et al. 2022. Front Immunol. 13:1011125. PubMed
  28. Horikoshi M, et al. 2012. PLoS One. 7:e51215. PubMed
  29. Ma L, et al. 2015. Blood. 126: 247 - 256. PubMed
  30. Wigton E, et al. 2016. J Leukoc Biol. 100: 143 - 153. PubMed
  31. Zhang B, et al. 2016. Mol Cell. 63: 976-89. PubMed
  32. Liang Z, et al. 2020. Pain. 161:2089. PubMed
  33. Riffelmacher T et al. 2017. Immunity. 47(3):466-480 . PubMed
  34. Fujita K, et al. 2019. Proc Natl Acad Sci U S A. 116:14714. PubMed
  35. Johnson MO et al. 2018. Cell. 175(7):1780-1795 . PubMed
  36. Socodato R, et al. 2020. Sci Signal. 13: . PubMed
  37. Wang X, et al. 2018. Mol Med Rep. 17:1660. PubMed
  38. Jasti S, et al. 2017. Biol Reprod. 96:134. PubMed
  39. Yue X, et al. 2019. Nat Commun. 10:2011. PubMed
  40. Wang W, et al. 2020. Immunity. 51(6):1102-1118.e7.. PubMed
  41. Xu H, et al. 2020. Immunity. 51(4):696-708.e9.. PubMed
  42. Witkowski MT, et al. 2020. Cancer Cell. 37:867. PubMed
  43. Knocke S, et al. 2016. Cell Rep. 17:2234-2246. PubMed
  44. Lewis EL, et al. 2021. Front Immunol. 12:741518. PubMed
  45. Cook KD, et al. 2015. Immunity. 43:703-714. PubMed
  46. Lewandowski JP, et al. 2020. Genome Biol. 1.039583333. PubMed
  47. Fang H, et al. 2020. Nat Commun. 4.661805556. PubMed
  48. Ko S, et al. 2009. J Virol. 83:748. PubMed
  49. Nahrendorf W, et al. 2021. eLife. 10:00. PubMed
  50. Cai Z, et al. 2020. Cell Rep. 31:107816. PubMed
  51. Luck H, et al. 2019. Nat Commun. 10:3650. PubMed
  52. Metidji A et al. 2018. Immunity. 49(2):353-362 . PubMed
  53. Olson B, et al. 2021. Brain Behav Immun. 97:102. PubMed
  54. Olive V, et al. 2013. Elife. 2:822. PubMed
  55. Ligon MM, et al. 2020. Mucosal Immunol. 1.172222222. PubMed
  56. Jing Y, et al. 2021. Front Immunol. 12:651860. PubMed
  57. Baglaenko Y, et al. 2016. PLoS One. 11: 0150515. PubMed
  58. Bazett M, et al. 2012. J Immunol. 188:2297. PubMed
  59. Amezcua Vesely MC, et al. 2020. Cell. 178(5):1176-1188.e15.. PubMed
  60. Wang X, et al. 2021. Cell. 184:5357. PubMed
  61. Alenazy MF, et al. 2022. PLoS One. 17:e0271689. PubMed
  62. Kim MY, et al. 2022. Nat Commun. 13:3296. PubMed
  63. Liu J, et al. 2015. Sci Rep. 5: 13558. PubMed
  64. Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed
  65. Cao A, et al. 2018. Nat Commun. 9:3288. PubMed
  66. Kimura S, et al. 2020. Am J Transplant. 20:977. PubMed
  67. Li S, et al. 2016. Sci Rep. 6:20425. PubMed
  68. Pelly V, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.4. PubMed
  69. Aalipour A, et al. 2020. Mol Ther Oncolytics. 17:232. PubMed
  70. Kunishita Y, et al. 2020. Front Immunol. 11:98. PubMed
  71. Rusconi M, et al. 2017. Sci Rep. 5.745138889. PubMed
  72. Che N, et al. 2021. Front Immunol. 12:626310. PubMed
  73. Rappe JCF, et al. 2021. J Exp Med. 218:. PubMed
  74. Chow AK, et al. 2021. Cellular and Molecular Gastroenterology and Hepatology. :. PubMed
  75. Omenetti S, et al. 2019. Immunity. 51:77. PubMed
  76. Hester AK, et al. 2022. Cell Rep. 38:110363. PubMed
  77. Amend A, et al. 2021. Int J Mol Sci. 22:. PubMed
RRID
AB_313646 (BioLegend Cat. No. 115511)
AB_313646 (BioLegend Cat. No. 115512)

Antigen Details

Structure
Ig superfamily, associates with CD21 and CD81, 95 kD
Distribution

Pro-B cells to mature B cells (during development), follicular dendritic cells

Function
Modulates B cell activation and differentiation
Ligand/Receptor
CD21, CD81, Leu-13
Cell Type
B cells, Dendritic cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341.
2. Krop I, et al. 1996. Eur. J. Immunol. 26:238.
3. Krop I, et al. 1996. J. Immunol. 157:48.
4. Tedder TF, et al. 1994. Immunol. Today 15:437.

Gene ID
12478 View all products for this Gene ID
UniProt
View information about CD19 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD19 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse CD19 6D5 FC
Biotin anti-mouse CD19 6D5 FC
FITC anti-mouse CD19 6D5 FC
PE anti-mouse CD19 6D5 FC
PE/Cyanine5 anti-mouse CD19 6D5 FC
Purified anti-mouse CD19 6D5 FC,CyTOF®,IHC-F
PE/Cyanine7 anti-mouse CD19 6D5 FC
Alexa Fluor® 488 anti-mouse CD19 6D5 FC
Alexa Fluor® 647 anti-mouse CD19 6D5 FC,IHC-F
Pacific Blue™ anti-mouse CD19 6D5 FC
Alexa Fluor® 700 anti-mouse CD19 6D5 FC
APC/Cyanine7 anti-mouse CD19 6D5 FC
PerCP anti-mouse CD19 6D5 FC
PerCP/Cyanine5.5 anti-mouse CD19 6D5 FC
Alexa Fluor® 594 anti-mouse CD19 6D5 IHC-F,FC
Brilliant Violet 421™ anti-mouse CD19 6D5 FC,IHC-F
Brilliant Violet 570™ anti-mouse CD19 6D5 FC
Brilliant Violet 605™ anti-mouse CD19 6D5 FC
Brilliant Violet 650™ anti-mouse CD19 6D5 FC
Brilliant Violet 785™ anti-mouse CD19 6D5 FC
Brilliant Violet 510™ anti-mouse CD19 6D5 FC
Purified anti-mouse CD19 (Maxpar® Ready) 6D5 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD19 6D5 FC
Brilliant Violet 711™ anti-mouse CD19 6D5 FC
APC/Fire™ 750 anti-mouse CD19 6D5 FC
TotalSeq™-A0093 anti-mouse CD19 6D5 PG
Brilliant Violet 750™ anti-mouse CD19 6D5 FC
TotalSeq™-B0093 anti-mouse CD19 6D5 PG
Spark Blue™ 550 anti-mouse CD19 6D5 FC
Spark NIR™ 685 anti-mouse CD19 6D5 FC
TotalSeq™-C0093 anti-mouse CD19 6D5 PG
Ultra-LEAF™ Purified anti-mouse CD19 6D5 FC,CyTOF®,IHC-F
PE/Fire™ 640 anti-mouse CD19 Antibody 6D5 FC
Spark YG™ 581 anti-mouse CD19 6D5 FC
APC/Fire™ 810 anti-mouse CD19 6D5 FC
Spark YG™ 570 anti-mouse CD19 6D5 IHC-F
Spark Blue™ 574 anti-mouse CD19 Antibody 6D5 FC
Spark Blue™ 515 anti-mouse CD19 6D5 FC
Spark UV™ 387 anti-mouse CD19 6D5 FC
Spark Red™ 718 anti-mouse CD19 (Flexi-Fluor™) 6D5 FC
Go To Top Version: 2    Revision Date: 05/24/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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