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PerCP/Cyanine5.5 anti-mouse CD19 Antibody

Pricing & Availability
Clone
6D5 (See other available formats)
Regulatory Status
RUO
Other Names
B4
Isotype
Rat IgG2a, κ
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Product Citations
publications
6D5_PerCPCyanine55_CD19_Antibody_102318
C57BL/6 mouse splenocytes were stained with CD3ε FITC and CD19 (clone 6D5) PerCP/Cyanine5.5 (left), or Rat IgG2a, κ PerCP/Cyanine5.5 isotype control (right).
  • 6D5_PerCPCyanine55_CD19_Antibody_102318
    C57BL/6 mouse splenocytes were stained with CD3ε FITC and CD19 (clone 6D5) PerCP/Cyanine5.5 (left), or Rat IgG2a, κ PerCP/Cyanine5.5 isotype control (right).
Compare all formats See PerCP/Cyanine5.5 spectral data
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115533 25 µg $89
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115534 100 µg $288
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Description

CD19 is a 95 kD glycoprotein also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81, forms a molecular complex integral to B cell activation.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CD19-expressing K562 human erythroleukemia cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence7.

Application References

(PubMed link indicates BioLegend citation)
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  2. Goodyear CS, et al. 2004. J. Immunol. 172:2870. (FC)
  3. Kamimura D, et al. 2006. J. Immunol. 177:306. (FC)
  4. Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (FC)
  5. Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC)
  6. Phan TG, et al. 2007. Nat. Immunol. 8:992. (FC)
  7. Hayashida K, et al. 2008. J. Biol. Chem. 283:19895. (IF) PubMed
  8. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  9. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  10. Stadnisky MD, et al. 2011. Blood. 117:5133. (FC) PubMed
  11. Perlot T, et al. 2012. J. Immunol. 188:1201. (FC) PubMed
  12. Olive V, et al. 2013. Elife. 2:822. PubMed
  13. Miyai T, et al. 2014. PNAS. 111:11780. PubMed
Product Citations
  1. Bradley CP et al. 2017. Cell host & microbe. 22(5):697-704 . PubMed
  2. Lang J, et al. 2020. Nat Commun. 11:1338. PubMed
  3. Fan L, et al. 2020. J Immunol Res. 2020:3032425. PubMed
  4. Mills EL, et al. 2022. Cell Metab. 34:140. PubMed
  5. Laffleur B, et al. 2022. Sci Immunol. 7:eabn2738. PubMed
  6. Arimoto KI, et al. 2023. Nat Commun. 14:251. PubMed
  7. Kulkarni R, et al. 2023. Life Sci Alliance. 6: . PubMed
  8. Potempa M, et al. 2022. J Immunol. 208:1742. PubMed
  9. Deng G, et al. 2023. Cancers (Basel). 15:. PubMed
  10. Miller CM, et al. 2020. J Virol. 94:00:00. PubMed
  11. Zhou J, et al. 2019. Immunity. 50:403. PubMed
  12. Frisbee AL, et al. 2019. Nat Commun. 10:2712. PubMed
  13. Webb LMC, et al. 2021. Aging Cell. 20:e13295. PubMed
  14. Lancaster JN, et al. 2019. Nat Commun. 10:2220. PubMed
  15. Kobayashi T, et al. 2019. Cell. 176:982. PubMed
  16. Smith D, et al. 2011. PLoS One. 7:e1002284. PubMed
  17. Wei H, et al. 2021. Malar J. 20:89. PubMed
  18. Guzmán M, et al. 2021. Mucosal Immunol. 14:1347. PubMed
  19. Yousif AS, et al. 2020. Immunity. 54(2):235-246.e5. PubMed
  20. Zhu X, et al. 2017. Arch Oral Biol. 10.1016/j.archoralbio.2017.03.010. PubMed
  21. Eastman A, et al. 2016. J Immunol . 194: 5999-6010. PubMed
  22. Wang X, et al. 2016. PLoS Pathog. 12: 1005367. PubMed
  23. Woodruff MC et al. 2018. Cell reports. 25(2):321-327 . PubMed
  24. Bratti M, et al. 2022. Front Immunol. 13:1029759. PubMed
  25. Gopinathan G, et al. 2022. Mol Cancer Ther. 21:1030. PubMed
  26. Waide ML, et al. 2020. Cell Rep. 33:108503. PubMed
  27. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  28. Santiago M, et al. 2010. J Immunol. 185:1114. PubMed
  29. Yoshihara E, et al. 2020. Nature. 606:586. PubMed
  30. Fachi JL, et al. 2020. J Exp Med. 217:00:00. PubMed
  31. Sochalska M, et al. 2016. Oncogene. 10.1038/onc.2016.362. PubMed
  32. Cuff AO, et al. 2020. Frontiers in Immunology. 1.930555556. PubMed
  33. Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed
  34. Krementsov DN, et al. 2018. Front Immunol. 9:1622. PubMed
  35. Palathingal Bava E, et al. 2022. JCI Insight. 7:. PubMed
  36. Wagage S, et al. 2014. J Immunol. 192:1661. PubMed
  37. Daniel CJ, et al. 2022. Mol Cancer Res. 20:1151. PubMed
  38. McFarland AP, et al. 2021. Immunity. 54(6):1320-1337.e4. PubMed
  39. Sangesland M, et al. 2020. Cell Reports. 32(8):108065. PubMed
  40. Uddin MJ, et al. 2022. Mucosal Immunol. 15:165. PubMed
  41. White C, et al. 2015. J Immunol. 194:697. PubMed
  42. Ariyoshi Y, et al. 2016. Gene Ther. 10.1038/gt.2016.7. PubMed
  43. Faust HJ, et al. 2020. J Clin Invest. 130:5493. PubMed
  44. Zhu H, et al. 2019. Nat Commun. 10:1084. PubMed
  45. LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed
  46. Denny JE, et al. 2019. Sci Rep. 2.786111111. PubMed
  47. Ding P, et al. 2022. Bone Res. 10:42. PubMed
  48. Hajas A, et al. 2013. Hum Immunol. 19:93. PubMed
  49. DiToro D, et al. 2020. Immunity. 650:52. PubMed
  50. Kim DK, et al. 2022. Nat Commun. 13:6292. PubMed
  51. Sakamoto K, et al. 2021. Immunity. 54:2321. PubMed
  52. Holzki J, et al. 2015. J Virol. 89: 9886 - 9895. PubMed
  53. Isvoranu G, et al. 2019. Oncol Lett. 17:4197. PubMed
  54. Maniati E, et al. 2020. Cell Rep. 30:525. PubMed
  55. Glasenapp A, et al. 2021. Circ Res. 129:369. PubMed
  56. Ghezraoui H, et al. 2018. Nature. 560:122. PubMed
  57. Li S, et al. 2013. Virology. 443:143. PubMed
  58. Velazquez VM, et al. 2017. Mol Ther Methods Clin Dev. 0.277083333. PubMed
  59. Royzman D, et al. 2019. Front Immunol. 10:633. PubMed
  60. Heil J, et al. 2021. Nat Commun. 12:6963. PubMed
  61. Fedele C, et al. 2021. J Exp Med. 218: . PubMed
  62. Li S, et al. 2014. J Immunol. 193:306. PubMed
  63. Peng V, et al. 2022. Nat Immunol. 23:619. PubMed
  64. Sakamoto K, et al. 2022. STAR Protoc. 3:101052. PubMed
  65. Ioanna E Galani et al. 2017. Immunity. 46(5):875-890 . PubMed
  66. Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed
RRID
AB_2072925 (BioLegend Cat. No. 115533)
AB_2072925 (BioLegend Cat. No. 115534)

Antigen Details

Structure
Ig superfamily, associates with CD21 and CD81, 95 kD
Distribution

Pro-B cells to mature B cells (during development), follicular dendritic cells

Function
Modulates B cell activation and differentiation
Ligand/Receptor
CD21, CD81, Leu-13
Cell Type
B cells, Dendritic cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341.
2. Krop I, et al. 1996. Eur. J. Immunol. 26:238.
3. Krop I, et al. 1996. J. Immunol. 157:48.
4. Tedder TF, et al. 1994. Immunol. Today 15:437.

Gene ID
12478 View all products for this Gene ID
UniProt
View information about CD19 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Other Formats

View All CD19 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse CD19 6D5 FC
Biotin anti-mouse CD19 6D5 FC
FITC anti-mouse CD19 6D5 FC
PE anti-mouse CD19 6D5 FC
PE/Cyanine5 anti-mouse CD19 6D5 FC
Purified anti-mouse CD19 6D5 FC,CyTOF®,IHC-F
PE/Cyanine7 anti-mouse CD19 6D5 FC
Alexa Fluor® 488 anti-mouse CD19 6D5 FC
Alexa Fluor® 647 anti-mouse CD19 6D5 FC,IHC-F
Pacific Blue™ anti-mouse CD19 6D5 FC
Alexa Fluor® 700 anti-mouse CD19 6D5 FC
APC/Cyanine7 anti-mouse CD19 6D5 FC
PerCP anti-mouse CD19 6D5 FC
PerCP/Cyanine5.5 anti-mouse CD19 6D5 FC
Alexa Fluor® 594 anti-mouse CD19 6D5 IHC-F,FC
Brilliant Violet 421™ anti-mouse CD19 6D5 FC,IHC-F
Brilliant Violet 570™ anti-mouse CD19 6D5 FC
Brilliant Violet 605™ anti-mouse CD19 6D5 FC
Brilliant Violet 650™ anti-mouse CD19 6D5 FC
Brilliant Violet 785™ anti-mouse CD19 6D5 FC
Brilliant Violet 510™ anti-mouse CD19 6D5 FC
Purified anti-mouse CD19 (Maxpar® Ready) 6D5 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD19 6D5 FC
Brilliant Violet 711™ anti-mouse CD19 6D5 FC
APC/Fire™ 750 anti-mouse CD19 6D5 FC
TotalSeq™-A0093 anti-mouse CD19 6D5 PG
Brilliant Violet 750™ anti-mouse CD19 6D5 FC
TotalSeq™-B0093 anti-mouse CD19 6D5 PG
Spark Blue™ 550 anti-mouse CD19 6D5 FC
Spark NIR™ 685 anti-mouse CD19 6D5 FC
TotalSeq™-C0093 anti-mouse CD19 6D5 PG
Ultra-LEAF™ Purified anti-mouse CD19 6D5 FC,CyTOF®,IHC-F
PE/Fire™ 640 anti-mouse CD19 Antibody 6D5 FC
Spark YG™ 581 anti-mouse CD19 6D5 FC
APC/Fire™ 810 anti-mouse CD19 6D5 FC
Spark YG™ 570 anti-mouse CD19 6D5 IHC-F
Spark Blue™ 574 anti-mouse CD19 Antibody 6D5 FC
Spark Blue™ 515 anti-mouse CD19 6D5 FC
Spark UV™ 387 anti-mouse CD19 6D5 FC
Spark Red™ 718 anti-mouse CD19 (Flexi-Fluor™) 6D5 FC
Go To Top Version: 3    Revision Date: 10/23/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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