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APC anti-mouse CD326 (Ep-CAM) Antibody

Pricing & Availability
Clone
G8.8 (See other available formats)
Regulatory Status
RUO
Other Names
CD326, EGP40, MIC18, TROP1, KSA
Isotype
Rat IgG2a, κ
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Product Citations
publications
G8dot8_APC_081908.jpg
TE-71 (mouse thymic epithelial stromal cell line) stained with G8.8 APC
  • G8dot8_APC_081908.jpg
    TE-71 (mouse thymic epithelial stromal cell line) stained with G8.8 APC
Compare all formats See APC spectral data
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118213 25 µg $102
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118214 100 µg $296
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Description

EpCAM (CD326) mediates calcium-independent homophilic cell to cell adhesion. It may also function as a growth factor receptor. It is thought to be involved in maintaining cells in position during proliferation. Expression of EpCAM seems to correlate inversely with the level of E-cadherin (CD324). EpCAM is considered important in tumor biology.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
TE-71 thymic epithelial cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications for clone G8.8 (for the relevant formats) include: immunohistochemistry of frozen sections: acetone fixed1, with or without OCT embedding2,4, and spatial biology (IBEX)13,14.

Application References

(PubMed link indicates BioLegend citation)
  1. Farr A, et al. 1991. J. Histochem. Cytochem. 39:645. (FC, IHC)
  2. Dooley J, et al. 2005. J. Immunol. 175:4331. (FC, IHC)
  3. Hinterberger M, et. al. 2010. Nat. Immunol. 11:512. (FC) PubMed
  4. Gracz AD, et al. 2010. Am J. Physiol Gastrointest Liver Physiol. 298:590. (IHC) PubMed
  5. Nudel I, et al. 2011. J. Immunol. 186:891. PubMed
  6. Morimoto H, et al. 2012. Biol Reprod. 86:148. PubMed
  7. Ishii K, et al. 2012. Development. 139:1734. PubMed
  8. Takehashi M, et al. 2012. Biol Reprod. 86:178. PubMed
  9. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  10. Taguchi K, et al. 2014. Mol Cell Biol. 34:900. PubMed
  11. Hirokawa Y, et al. 2014. Am J Physiol Gastrointerest Liver Physiol. 306:547. PubMed
  12. Ding X, et al. 2015. Cancer Res. 75:330. PubMed
  13. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  14. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Garo LP, et al. 2021. Nat Commun. 12:2419. PubMed
  2. Hohmann MS, et al. 2021. JCI Insight. 6:. PubMed
  3. Zhu Y, et al. 2022. Nat Commun. 13:4282. PubMed
  4. Park SE, et al. 2022. Cell Mol Gastroenterol Hepatol. 14:1235. PubMed
  5. Na E, et al. 2022. Am J Physiol Lung Cell Mol Physiol. 322:L550. PubMed
  6. Xu J, et al. 2022. Dev Cell. 57:839. PubMed
  7. Serra-Hassoun M, et al. 2014. J Immunol. 193:1504. PubMed
  8. Elkon R, et al. 2015. Nat Commun. 6: 8549. PubMed
  9. Bowling S, et al. 2020. Cell. 181(6):1410-1422.e27. PubMed
  10. Sun L, et al. 2021. Cancer Cell. 39:1361. PubMed
  11. Chen Q, et al. 2020. Cell Reports. 31(13):107828. PubMed
  12. Moriyama M, et al. 2023. Proc Natl Acad Sci U S A. 120:e2221652120. PubMed
  13. Lv X, et al. 2023. Acta Pharm Sin B. 13:1631. PubMed
  14. Cui W, et al. 2023. J Exp Med. 220:. PubMed
  15. Kalgudde Gopal S, et al. 2023. Nat Commun. 14:3020. PubMed
  16. Lv X, et al. 2022. Acta Pharm Sin B. 12:735. PubMed
  17. Boyd DF, et al. 2020. Nature. 587:466. PubMed
  18. Mevel R, et al. 2020. eLife. 9:00. PubMed
  19. Buerger S, et al. 2015. J Immunol. 195: 4106 - 4116. PubMed
  20. Malhotra D, et al. 2016. Nat Immunol. 17:187-195. PubMed
  21. Cheung P, et al. 2020. Cell Stem Cell. 27(4):590-604.e9. PubMed
  22. Komban RJ, et al. 2019. Nat Commun. 10:2423. PubMed
  23. Kato T, et al. 2019. Front Immunol. 10:2021. PubMed
  24. Ford MJ, et al. 2021. Cell Rep. 36:109677. PubMed
  25. Shultz L, et al. 2010. Proc Natl Acad Sci U S A. 107:13022. PubMed
  26. Paris AJ, et al. 2020. Nat Cell Biol. 1.747916667. PubMed
  27. Nishina T, et al. 2021. Nat Commun. 12:2281. PubMed
  28. Phung HT, et al. 2020. Immunohorizons. 0.25625. PubMed
  29. Semba T, et al. 2020. Cancers (Basel). 12:. PubMed
  30. Chua CW, et al. 2018. Elife. 7. PubMed
  31. Finn J et al. 2019. Cell reports. 26(11):2942-2954 . PubMed
  32. Willobee BA, et al. 2021. Mol Cancer Ther. 20:1246. PubMed
  33. Ludwik KA, et al. 2021. STAR Protocols. 2(1):100270. PubMed
  34. Santos CP et al. 2019. Nat Commun. 10(1):4407 . PubMed
  35. Goldfarb Y, et al. 2021. J Exp Med. 218:. PubMed
  36. Wang Y, et al. 2014. PLoS One. 9:113127. PubMed
  37. Katara GK, et al. 2020. Mol Oncol. 0.729861111. PubMed
  38. Malik A et al. 2018. Immunity. 49(3):515-530 . PubMed
  39. Rudnick JA, et al. 2021. Genes Dev. 35:963. PubMed
  40. Lüönd F, et al. 2022. STAR Protoc. 3:101438. PubMed
  41. Wu H, et al. 2021. Cell Death Discov. 7:225. PubMed
  42. Liu S, et al. 2020. Cell Host & Microbe. 26(6):779-794.e8.. PubMed
  43. Jiang M, et al. 2021. Developmental Cell. 56(11):1646-1660.e5. PubMed
  44. Barone F, et al. 2015. Proc Natl Acad Sci U S A. 112: 11024-11029. PubMed
  45. Bar C, et al. 2020. Cell Reports. 28(1):257-266.e5.. PubMed
  46. Tuong ZK, et al. 2021. iScience. 24:103326. PubMed
  47. Ionkina AA, et al. 2021. Breast Cancer Res. 23:93. PubMed
  48. Zhang Z, et al. 2021. Exp Dermatol. 30:102. PubMed
  49. Hertzano R, et al. 2020. Laryngoscope. . PubMed
  50. Xi‐Zhi J Guo et al. 2018. Immunity. 49(3):531-544 . PubMed
  51. Sateriale A, et al. 2020. Cell Host & Microbe. 26(1):135-146.e5.. PubMed
  52. Li J, et al. 2020. Development. :. PubMed
  53. Cerretelli G, et al. 2021. J Pathol. 255:464. PubMed
  54. Tang Z, et al. 2021. Stem Cell Res Ther. 12:397. PubMed
  55. Christin JR, et al. 2020. Cell Reports. 31(10):107742. PubMed
  56. McGinty JW, et al. 2020. Immunity. 52(3):528-541. PubMed
  57. Gerdes P, et al. 2022. Nat Commun. 13:7470. PubMed
  58. Ualiyeva S, et al. 2021. Bio Protoc. 11:e4163. PubMed
  59. Ognjenovic NB, et al. 2020. Dev Cell. 55:544. PubMed
  60. Zhang Y, et al. 2021. Commun Biol. 344:4. PubMed
  61. Li MY, et al. 2021. Developmental Cell. 56:2547. PubMed
  62. Caporarello N, et al. 2020. Aging Cell. 19:e13196. PubMed
  63. Pepe‐Mooney BJ et al. 2019. Cell Stem Cell. 25(1):23-38 . PubMed
  64. Smith NR, et al. 2018. Cell Mol Gastroenterol Hepatol. 6:79. PubMed
  65. King J, et al. 2012. Am J Physiol Gastrointest Liver Physiol. 303:443. PubMed
  66. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  67. Schmit T, et al. 2022. Cell Rep. 38:110456. PubMed
  68. Chetty A, et al. 2021. Cell Host Microbe. 29:579. PubMed
  69. Baptista M, et al. 2016. Nat Commun. 7:12175. PubMed
  70. Castillo–Azofeifa D, et al. 2019. EMBO J. 38:e99984. PubMed
  71. Tippimanchai DD, et al. 2018. Oncoimmunology. 7:e1438105. PubMed
  72. Caporarello N, et al. 2022. Nat Commun. 13:4170. PubMed
  73. Casey O, et al. 2012. PLoS One. 7:e41668. PubMed
  74. Tanimizu N, et al. 2014. J Biol Chem. 289:7859. PubMed
  75. Kasuga A, et al. 2020. Cancer Sci. . PubMed
  76. Gross KM, et al. 2019. Cell Rep. 28:394. PubMed
  77. Lai NY, et al. 2020. Cell. 180:33:00. PubMed
  78. Lehmann M, et al. 2020. Cell Signal. 70:109588. PubMed
  79. Chen Z, et al. 2020. Immunity. 54(1):151-163.e6. PubMed
  80. Tripathi D, et al. 2016. Nat Commun. 7:13896. PubMed
  81. Han H, et al. 2020. Cancer Cell. 36(5):483-497.e15.. PubMed
  82. Jadhav U, et al. 2019. Mol Cell. 74:542. PubMed
  83. Renz BW, et al. 2018. Cancer Cell. 33:75. PubMed
  84. Cohen I et al. 2018. Cell stem cell. 22(5):726-739 . PubMed
RRID
AB_1134102 (BioLegend Cat. No. 118213)
AB_1134102 (BioLegend Cat. No. 118214)

Antigen Details

Structure
40 kD single-pass type 1 glycoprotein. 293 amino acids, with a 21 aa signal peptide, a 246 aa extracellular domain, a 21 aa transmembrane domain, and a 26 aa cytoplasmic domain. The extracellular domain contains two epidermal growth factor-like repeats.
Distribution

Expressed on majority of epithelial cell membranes with the exception of adult squamous cells of the skin and a few specific epithelial cell types.

Function
Mediates calcium-independent homophilic cell-cell adhesion.
Interaction
CD326 displays hemophilic binding.
Ligand/Receptor
CD305 (LAIR-1), CD306 (LAIR-2), and Ep-CAM.
Cell Type
Embryonic Stem Cells, Epithelial cells
Biology Area
Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Borkowski TA, et al. 1996. Eur. J. Immunol. 26:110.
2. Bergsagel PL, et al. 1992. J. Immunol. 148:590.

Gene ID
17075 View all products for this Gene ID
UniProt
View information about CD326 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD326 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse CD326 (Ep-CAM) G8.8 FC
Purified anti-mouse CD326 (Ep-CAM) G8.8 FC,IHC-F,ICC
Biotin anti-mouse CD326 (Ep-CAM) G8.8 FC
PE anti-mouse CD326 (Ep-CAM) G8.8 FC
FITC anti-mouse CD326 (Ep-CAM) G8.8 FC
Alexa Fluor® 488 anti-mouse CD326 (Ep-CAM) G8.8 FC,IHC-F,3D IHC
Alexa Fluor® 647 anti-mouse CD326 (Ep-CAM) G8.8 FC,IHC-F,3D IHC,SB
PE/Cyanine7 anti-mouse CD326 (Ep-CAM) G8.8 FC
APC/Cyanine7 anti-mouse CD326 (Ep-CAM) G8.8 FC
PerCP/Cyanine5.5 anti-mouse CD326 (Ep-CAM) G8.8 FC
Alexa Fluor® 594 anti-mouse CD326 (Ep-CAM) G8.8 ICC,IHC-F,3D IHC
Brilliant Violet 421™ anti-mouse CD326 (Ep-CAM) G8.8 FC
Brilliant Violet 605™ anti-mouse CD326 (Ep-CAM) G8.8 FC
Purified anti-mouse CD326 (Ep-CAM) (Maxpar® Ready) G8.8 FC,CyTOF®
APC/Fire™ 750 anti-mouse CD326 (Ep-CAM) G8.8 FC
Brilliant Violet 711™ anti-mouse CD326 (Ep-CAM) G8.8 FC
Brilliant Violet 510™ anti-mouse CD326 (Ep-CAM) G8.8 FC
PE/Dazzle™ 594 anti-mouse CD326 (Ep-CAM) G8.8 FC
TotalSeq™-A0449 anti-mouse CD326 (Ep-CAM) G8.8 PG
Alexa Fluor® 700 anti-mouse CD326 (Ep-CAM) G8.8 FC
TotalSeq™-C0449 anti-mouse CD326 (Ep-CAM) G8.8 PG
Brilliant Violet 785™ anti-mouse CD326 (Ep-CAM) G8.8 FC
TotalSeq™-B0449 anti-mouse CD326 (Ep-CAM) G8.8 PG
Brilliant Violet 650™ anti-mouse CD326 (Ep-CAM) G8.8 FC
PE/Cyanine5 anti-mouse CD326 (Ep-CAM) G8.8 FC
Spark Red™ 718 anti-mouse CD326 (Ep-CAM) (Flexi-Fluor™) G8.8 FC
Spark Blue™ 574 anti-mouse CD326 (Ep-CAM) (Flexi-Fluor™) G8.8 FC
Spark Blue™ 550 anti-mouse CD326 (Ep-CAM) (Flexi-Fluor™) G8.8 FC
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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