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PE anti-mouse CD326 (Ep-CAM) Antibody

Pricing & Availability
Clone
G8.8 (See other available formats)
Regulatory Status
RUO
Other Names
CD326, EGP40, MIC18, TROP1, KSA
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
G8.8_PE_CD326(EpCAM)_Antibody_1_FC_100416
TE-71 (mouse thymic epithelial stromal cell line) cells were stained with CD326 (clone G8.8) PE (filled histogram) or rat IgG2a, κ PE isotype control (open histogram).
  • G8.8_PE_CD326(EpCAM)_Antibody_1_FC_100416
    TE-71 (mouse thymic epithelial stromal cell line) cells were stained with CD326 (clone G8.8) PE (filled histogram) or rat IgG2a, κ PE isotype control (open histogram).
Compare all formats See PE spectral data
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118205 50 µg $102
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118206 200 µg $329
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Description

EpCAM (CD326) mediates calcium-independent homophilic cell to cell adhesion. It may also function as a growth factor receptor. It is thought to be involved in maintaining cells in position during proliferation. Expression of EpCAM seems to correlate inversely with the level of E-cadherin (CD324). EpCAM is considered important in tumor biology.

Product Details
Technical data sheet

Product Details

Verified Reactivity
mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
TE-71 thymic epithelial cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications for clone G8.8 (for the relevant formats) include: immunohistochemistry of frozen sections: acetone fixed1, with or without OCT embedding2,4, and spatial biology (IBEX)13,14.

Application References

(PubMed link indicates BioLegend citation)
  1. Farr A, et al. 1991. J. Histochem. Cytochem. 39:645. (FC, IHC)
  2. Dooley J, et al. 2005. J. Immunol. 175:4331. (FC, IHC)
  3. Hinterberger M, et. al. 2010. Nat. Immunol. 11:512. (FC) PubMed
  4. Gracz AD, et al. 2010. Am J. Physiol Gastrointest Liver Physiol. 298:590. (IHC) PubMed
  5. Nudel I, et al. 2011. J. Immunol. 186:891. PubMed
  6. Morimoto H, et al. 2012. Biol Reprod. 86:148. PubMed
  7. Ishii K, et al. 2012. Development. 139:1734. PubMed
  8. Takehashi M, et al. 2012. Biol Reprod. 86:178. PubMed
  9. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  10. Taguchi K, et al. 2014. Mol Cell Biol. 34:900. PubMed
  11. Hirokawa Y, et al. 2014. Am J Physiol Gastrointerest Liver Physiol. 306:547. PubMed
  12. Ding X, et al. 2015. Cancer Res. 75:330. PubMed
  13. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  14. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Jiang W, et al. 2022. Redox Biol. 56:102432. PubMed
  2. Naranjo S, et al. 2022. Genes Dev. 36:936. PubMed
  3. Yang H, et al. 2023. Cell Death Differ. 30:560. PubMed
  4. Pennitz P 2022. STAR Protocols. 4(1):101957. PubMed
  5. Kimura S, et al. 2020. Nat Commun. 0.620833333. PubMed
  6. Gao Q, et al. 2019. J Exp Med. 216:688. PubMed
  7. Wang M, et al. 2022. Theranostics. 12:4513. PubMed
  8. Chiang HC, et al. 2018. Sci Rep. 8:2731. PubMed
  9. Lancaster JN, et al. 2019. Nat Commun. 10:2220. PubMed
  10. Nair S, et al. 2016. Nat Commun. 7:10913. PubMed
  11. Schwartz AJ, et al. 2021. Nat Metab. 3:969. PubMed
  12. Skibba ME, et al. 2021. Respir Res. 22:315. PubMed
  13. Valds-Mora F, et al. 2021. Cell Reports. 35(2):108945. PubMed
  14. Yin N et al. 2019. Cancer Cell. 36(2):156-167 . PubMed
  15. Hao F, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  16. Yu D, et al. 2013. Mol Cell Biol. 33:1845. PubMed
  17. Kim S, et al. 2011. PLoS One. 6:e19310. PubMed
  18. Parker KA, et al. 2021. Mol Cancer Res. 19:2068. PubMed
  19. Weiner AI, et al. 2019. NPJ Regen Med. 4:17. PubMed
  20. Bahar Halpern K, et al. 2020. Nat Commun. 11:1936. PubMed
  21. Airik M, et al. 2020. Hum Mol Genet. 29:3064. PubMed
  22. Reinfeld BI, et al. 2021. Nature. 593:282. PubMed
  23. Krueger A, et al. 2022. Front Microbiol. 12:789042. PubMed
  24. Scur M, et al. 2022. Nat Commun. 13:7272. PubMed
  25. Sun L, et al. 2015. Sci Rep. 5: 14871. PubMed
  26. Lee J, et al. 2013. Am J Respir Cell Mol Biol. 48:288. PubMed
  27. Tan C, et al. 2020. Front Cell Dev Biol. 8:579157. PubMed
  28. Yang Y, et al. 2021. Cell Reports. 34(10):108822. PubMed
  29. Mello AM, et al. 2022. Oncogenesis. 11:56. PubMed
  30. Tateishi R, et al. 2015. PLoS One. 10: e0141650. PubMed
  31. He B, et al. 2018. Nat Commun. 9:5240. PubMed
  32. Zheng C, et al. 2022. Acta Pharm Sin B. 12:3726. PubMed
  33. Li Q, et al. 2022. J Biol Chem. :101753. PubMed
  34. Shigeyasu K, et al. 2015. Gut. 64:627. PubMed
  35. Kudira R, et al. 2021. Bio Protoc. 11:e4192. PubMed
  36. Fujino N, et al. 2019. J Exp Med. 216:2184. PubMed
  37. Edelson B, et al. 2011. PLoS One. 6:e25660. PubMed
  38. Barr J, et al. 2022. Elife. 11:. PubMed
  39. Ichinose M, et al. 2021. Sci Rep. 7200:11. PubMed
  40. Avgustinova A, et al. 2021. Cell Stem Cell. . PubMed
  41. Pastor W, et al. 2014. Nat Commun. 12:5795. PubMed
  42. Filbey KJ, et al. 2019. Front Immunol. 10:2375. PubMed
RRID
AB_1134172 (BioLegend Cat. No. 118205)
AB_1134172 (BioLegend Cat. No. 118206)

Antigen Details

Structure
40 kD single-pass type 1 glycoprotein. 293 amino acids, with a 21 aa signal peptide, a 246 aa extracellular domain, a 21 aa transmembrane domain, and a 26 aa cytoplasmic domain. The extracellular domain contains two epidermal growth factor-like repeats.
Distribution

Expressed on majority of epithelial cell membranes with the exception of adult squamous cells of the skin and a few specific epithelial cell types.

Function
Mediates calcium-independent homophilic cell-cell adhesion.
Interaction
CD326 displays hemophilic binding.
Ligand/Receptor
CD305 (LAIR-1), CD306 (LAIR-2), and Ep-CAM.
Cell Type
Embryonic Stem Cells, Epithelial cells
Biology Area
Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Borkowski TA, et al. 1996. Eur. J. Immunol. 26:110.
2. Bergsagel PL, et al. 1992. J. Immunol. 148:590.

Gene ID
17075 View all products for this Gene ID
UniProt
View information about CD326 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.

Other Formats

View All CD326 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse CD326 (Ep-CAM) G8.8 FC
Purified anti-mouse CD326 (Ep-CAM) G8.8 FC,IHC-F,ICC
Biotin anti-mouse CD326 (Ep-CAM) G8.8 FC
PE anti-mouse CD326 (Ep-CAM) G8.8 FC
FITC anti-mouse CD326 (Ep-CAM) G8.8 FC
Alexa Fluor® 488 anti-mouse CD326 (Ep-CAM) G8.8 FC,IHC-F,3D IHC
Alexa Fluor® 647 anti-mouse CD326 (Ep-CAM) G8.8 FC,IHC-F,3D IHC,SB
PE/Cyanine7 anti-mouse CD326 (Ep-CAM) G8.8 FC
APC/Cyanine7 anti-mouse CD326 (Ep-CAM) G8.8 FC
PerCP/Cyanine5.5 anti-mouse CD326 (Ep-CAM) G8.8 FC
Alexa Fluor® 594 anti-mouse CD326 (Ep-CAM) G8.8 ICC,IHC-F,3D IHC
Brilliant Violet 421™ anti-mouse CD326 (Ep-CAM) G8.8 FC
Brilliant Violet 605™ anti-mouse CD326 (Ep-CAM) G8.8 FC
Purified anti-mouse CD326 (Ep-CAM) (Maxpar® Ready) G8.8 FC,CyTOF®
APC/Fire™ 750 anti-mouse CD326 (Ep-CAM) G8.8 FC
Brilliant Violet 711™ anti-mouse CD326 (Ep-CAM) G8.8 FC
Brilliant Violet 510™ anti-mouse CD326 (Ep-CAM) G8.8 FC
PE/Dazzle™ 594 anti-mouse CD326 (Ep-CAM) G8.8 FC
TotalSeq™-A0449 anti-mouse CD326 (Ep-CAM) G8.8 PG
Alexa Fluor® 700 anti-mouse CD326 (Ep-CAM) G8.8 FC
TotalSeq™-C0449 anti-mouse CD326 (Ep-CAM) G8.8 PG
Brilliant Violet 785™ anti-mouse CD326 (Ep-CAM) G8.8 FC
TotalSeq™-B0449 anti-mouse CD326 (Ep-CAM) G8.8 PG
Brilliant Violet 650™ anti-mouse CD326 (Ep-CAM) G8.8 FC
PE/Cyanine5 anti-mouse CD326 (Ep-CAM) G8.8 FC
Spark Red™ 718 anti-mouse CD326 (Ep-CAM) (Flexi-Fluor™) G8.8 FC
Spark Blue™ 574 anti-mouse CD326 (Ep-CAM) (Flexi-Fluor™) G8.8 FC
Spark Blue™ 550 anti-mouse CD326 (Ep-CAM) (Flexi-Fluor™) G8.8 FC
Go To Top Version: 2    Revision Date: 10/04/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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