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APC anti-mouse CD3ε Antibody

Pricing & Availability
Clone
145-2C11 (See other available formats)
Regulatory Status
RUO
Other Names
CD3ε, T3, CD3
Isotype
Armenian Hamster IgG
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Product Citations
publications
145-2C11_APC_021606
C57BL/6 mouse splenocytes were stained with CD3e (clone 145-2C11) APC (filled histogram) or Armenian hamster IgG APC isotype control (open histogram).
  • 145-2C11_APC_021606
    C57BL/6 mouse splenocytes were stained with CD3e (clone 145-2C11) APC (filled histogram) or Armenian hamster IgG APC isotype control (open histogram).
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100311 25 µg $33
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100312 100 µg $105
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Description

CD3ε is a 20 kD transmembrane protein, also known as CD3 or T3. It is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3ε forms a TCR complex by associating with the CD3δ, γ and ζ chains, as well as the TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
H-2Kb-specific mouse cytotoxic T lymphocyte clone BM10-37
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The CD3ε antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Clone 145-2C11 is useful for in vitro blocking of target-specific CTL-mediated cell lysis1, as well as T cell activation assays, inducing proliferation and cytokine production1,2,7,12,16. It also induces apoptosis in immature thymocytes32,  and in vivo T cell depletion8-10. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation1, immunohistochemical staining14,15 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, Western blotting4, complement-mediated cytotoxicity6, in vitro and in vivo stimulation of T cells1,2,7,12,16, immunofluorescent staining5, and in vivo T cell depletion8-10. The 145-2C11 antibody has been reported to block the binding of 17A2 antibody to CD3 epsilon-specific T cells11. Clone 145-2C11 is not recommended for formalin-fixed paraffin embedded sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 100314). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 100340) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Leo O, et al. 1987. P. Natl. Acad. Sci. USA 84:1374. (IP, Activ, Block)
  2. Kruisbeek AM, et al. 1991. In Current Protocols in Immunology. 3.12.1. (Activ)
  3. Duke RC, et al. 1995. Current Protocols in Immunology. 3.17.1.
  4. Salvadori S, et al. 1994. J. Immunol. 153:5176. (WB)
  5. Payer E, et al. 1991. J. Immunol. 146:2536. (IF)
  6. Jacobs H, et al. 1994. Eur. J. Immunol. 24:934. (CMCD)
  7. Vossen ACTM, et al. 1995. Eur. J. Immunol. 25:1492. (Activ)
  8. Henrickson M, et al. 1995. Transplantation 60:828. (Deplete)
  9. Kinnaert P, et al. 1996. Transpl. Int. 9:386. (Deplete)
  10. Han WR, et al. 1999. Transpl. Immunol. 7:207. (Deplete)
  11. Miescher GC, et al. 1989. Immunol. Lett. 23:113. (Block)
  12. Terrazas LI, et al. 2005. Intl. J. Parasitology. 35:1349. (Activ)
  13. Ko SY, et al. 2005. J. Immunol. 175:3309.
  14. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC-F)
  15. Tilley SL, et al. 2007. J. Immunol. 178:3208. (IHC-F)
  16. Wang W, et al. 2007. J. Immunol. 178:4885. (Activ)
  17. Xiao S, et al. 2007. J. Exp. Med. 204:1691.
  18. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed.
  19. Curtsinger JM, et al.2005. J. Immunol. 175:4392. PubMed
  20. Guo Y, et al. 2008. Blood 112:480. PubMed
  21. Kenna TJ, et al. 2008. Blood 111:2091.
  22. Perchonock CE, et al. 2007. J. Immunol. 179:1768. PubMed
  23. Perchonock GE, et al. 2006. Mol. Cell. Biol. 26:6005. PubMed
  24. Kanaya T, et al. 2008. Am. J. Physiol. Gastrointest. Liver Physiol. 295:G273. PubMed
  25. de Koning BA, et al. 2006. Int. Immunol. 18:941. PubMed
  26. Schulteis RD, et al. 2008. Blood 295:G273. PubMed
  27. Qi Q, et al. 2009. Blood 114:564. PubMed
  28. Helmersson S, et al. 2013. Am J Pathol. 9440:123. Pubmed
  29. Wu S, et al. 2014. Clin Vaccine Immunol. 21:156. PubMed
  30. Yan J, et al. 2014. Vaccine. 32:2833. PubMed
  31. Guiterrez DA, et al. 2014. Diaebetes. 63:3827. PubMed
  32. Shi YF, et al. 1991. J Immunol. 146:3340. (Apop)
Product Citations
  1. Barrett N, et al. 2016. Cell Rep. 16: 1039-1054. PubMed
  2. Glasner A,et al. 2017. Sci Rep.. 10.1038/s41598-017-12998-w. PubMed
  3. Chan LC, et al. 2019. J Clin Invest. 129:3324. PubMed
  4. Dong MB, et al. 2020. Cell. 178(5):1189-1204.e23.. PubMed
  5. Wang Q, et al. 2020. Radiat Environ Biophys. 59:89. PubMed
  6. Nelson J, et al. 2020. Sci Adv. 6:eaaz6893. PubMed
  7. Li H, et al. 2022. Cancer Cell. 40:36. PubMed
  8. Wang B, et al. 2022. EMBO J. 41:e108946. PubMed
  9. Koenig A, et al. 2022. Front Immunol. 12:791100. PubMed
  10. Cha JH, et al. 2022. J Biol Chem. 298:101817. PubMed
  11. Khayrullina G, et al. 2022. Glia. 70:1337. PubMed
  12. Gargaro M, et al. 2022. Immunity. 55:1032. PubMed
  13. Chen C, et al. 2022. Sci Adv. 8:eabo4363. PubMed
  14. Kumpunya S, et al. 2023. Front Immunol. 13:1010764. PubMed
  15. Sieow JL, et al. 2023. Int J Mol Sci. 24: . PubMed
  16. Agrafiotis A, et al. 2023. Acta Neuropathol. 145:335. PubMed
  17. Kemna J, et al. 2023. Nat Immunol. 24:414. PubMed
  18. Drijvers JM, et al. 2021. Cancer Immunol Res. 9:184. PubMed
  19. Sohn J, et al. 2022. Blood Adv. 6:2557. PubMed
  20. Zhu Z, et al. 2022. Clin Cancer Res. 28:4820. PubMed
  21. Zhuang WR, et al. 2023. Nat Commun. 14:1675. PubMed
  22. van Os BW, et al. 2023. Eur Heart J Open. 3:oead013. PubMed
  23. Seclì L, et al. 2023. J Immunother Cancer. 11:. PubMed
  24. van Os BW, et al. 2023. Front Cardiovasc Med. 10:1171764. PubMed
  25. Ferrari de Andrade L, et al. 2020. Cancer Immunol Res. 0.867361111. PubMed
  26. Pennock N, et al. 2014. Proc Natl Acad Sci U S A. 111:16472. PubMed
  27. Ajina R, et al. 2019. Oncoimmunology. 8:e1577127. PubMed
  28. Yamaguchi S, et al. 2021. Eur J Immunol. 51:2306. PubMed
  29. Yang Z, et al. 2022. J Immunother Cancer. 10:. PubMed
  30. Fujimori S, et al. 2022. Elife. 11:. PubMed
  31. Ma J, et al. 2020. Adv Sci (Weinh). 7:2000609. PubMed
  32. Symeonidou V, et al. 2021. Cell Rep. 37:109900. PubMed
  33. Okamoto M, et al. 2014. J Immunol. 192:2770. PubMed
  34. Nakamura M, et al. 2015. J Immunol. 194:243. PubMed
  35. Altshuler A, et al. 2021. Cell Stem Cell. 28(7):1248-1261.e8. PubMed
  36. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  37. Roufaiel M, et al. 2016. Nat Immunol. 10.1038/ni.3564. PubMed
  38. Ku M, et al. 2016. Mol Cancer Ther. 15: 1975 - 1987. PubMed
  39. Agelidis A, et al. 2019. Front Immunol. 10:500. PubMed
  40. Yang X, et al. 2020. Blood Sci. 2:89. PubMed
  41. Volpedo G, et al. 2022. NPJ Vaccines. 7:32. PubMed
  42. Wang Y, et al. 2016. Sci Rep. 6:31881. PubMed
  43. Mukherjee D, et al. 2022. Nat Commun. 13:3747. PubMed
  44. Yang W, et al. 2020. Mucosal Immunol. 13:788. PubMed
  45. Jung BG, et al. 2022. PLoS Pathog. 18:e1010454. PubMed
  46. Haugh KA, et al. 2021. eLife. 0.416666666666667. PubMed
  47. Sato K, et al. 2015. PLoS One. 10: 0138291. PubMed
  48. Lercher A, et al. 2019. Immunity. 51:1074. PubMed
  49. Gao JL, et al. 2019. JCI Insight. 4:e132140. PubMed
  50. Gorczynski RM, et al. 2017. Immunology. 150:418. PubMed
  51. Wang J, et al. 2018. Front Immunol. 9:371. PubMed
  52. Zhou H, et al. 2022. EMBO Rep. 23:e53475. PubMed
  53. Wan W, et al. 2015. Cardiovasc Res. 106:478. PubMed
  54. Montel-Hagen A, et al. 2020. Cell Rep. 33:108320. PubMed
  55. Fujita K, et al. 2019. Proc Natl Acad Sci U S A. 116:14714. PubMed
  56. Dane EL, et al. 2022. Nat Mater. 21:710. PubMed
  57. Leiva M, et al. 2016. Nat Commun. 7:10222. PubMed
  58. Holvoet B, et al. 2015. Stem Cell Reports. 5: 1183-1195. PubMed
  59. Burrack K, et al. 2015. PLoS Pathog. 11: e1005191. PubMed
  60. Lopez DA, et al. 2022. Cell Rep. 41:111677. PubMed
  61. Aebischer D, et al. 2014. PLoS One. 9:99297. PubMed
  62. Codina A, et al. 2019. Cell Syst. 8:136. PubMed
  63. Xu H, et al. 2020. Immunity. 51(4):696-708.e9.. PubMed
  64. Dong S, et al. 2021. Nature. 591:117. PubMed
  65. Ghandhi SA, et al. 2022. Sci Rep. 12:14124. PubMed
  66. Mansell E, et al. 2020. Cell Stem Cell. . PubMed
  67. Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed
  68. Ito C, et al. 2015. PLoS One. 10: e0140808. PubMed
  69. Siggs O, et al. 2015. Proc Natl Acad Sci U S A. 112: E5706 - E5714. PubMed
  70. Burrack K, et al. 2015. J Immunol. 194:678. PubMed
  71. Parhi L, et al. 2020. Nat Commun. 2.721527778. PubMed
  72. Hum NR, et al. 2022. Front Oncol. 12:928474. PubMed
  73. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  74. Luck H, et al. 2015. Cell Metab. 21 527 . PubMed
  75. Nosho S, et al. 2020. Int J Mol Sci. 21:00. PubMed
  76. Zhang H, et al. 2019. Mol Cell. 76:110. PubMed
  77. Oksenych V, et al. 2021. Biomolecules. 12:. PubMed
  78. Mittal A, et al. 2021. Sci Rep. 11:10731. PubMed
  79. Gu X, et al. 2022. Vaccines (Basel). 10:. PubMed
  80. Liu B, et al. 2022. Mol Med Rep. 26:. PubMed
  81. Uto T, et al. 2016. Nat Commun. 7: 11273. PubMed
  82. Rovira-Clavé X, et al. 2016. J Leukoc Biol. 99: 143 - 152. PubMed
  83. XS R, et al. 2015. Diabetes. 64 90. PubMed
  84. Liu Z, et al. 2020. Cell. 178(6):1509-1525.e19.. PubMed
  85. Ghandhi SA, et al. 2019. Sci Rep. 9:19434. PubMed
  86. Xia P et al. 2018. Immunity. 48(4):688-701 . PubMed
  87. Liu S, et al. 2018. Front Immunol. 29:208. PubMed
  88. Sun Y, et al. 2022. Mol Ther Oncolytics. 25:160. PubMed
  89. Huang J, et al. 2014. J Immunol. 192:1972. PubMed
  90. Gubin MM, et al. 2018. Cell. 175:1014. PubMed
  91. Zhang X, et al. 2021. Mol Cancer Res. 19:1076. PubMed
  92. Li D, et al. 2022. Emerg Microbes Infect. 11:2248. PubMed
  93. Liu Y, et al. 2022. Am J Transl Res. 14:6856. PubMed
  94. Nahrendorf W, et al. 2021. eLife. 10:00. PubMed
  95. Gong N, et al. 2020. Nat Nanotechnol. 1.35625. PubMed
  96. Metidji A et al. 2018. Immunity. 49(2):353-362 . PubMed
  97. Zhang CR, et al. 2022. Blood Cancer Discov. 3:220. PubMed
  98. Zhang Q, et al. 2022. JCI Insight. 7:. PubMed
  99. Wang Y, et al. 2021. Nat Commun. 12:4964. PubMed
  100. Wang W, et al. 2021. J Am Heart Assoc. 10:e019142. PubMed
  101. Berghoff SA, et al. 2021. Nat Neurosci. 24:47. PubMed
  102. Shibuya M, et al. 2021. iScience. 24:103131. PubMed
  103. Liang Q, et al. 2020. Front Microbiol. 11:571847. PubMed
  104. Miura N, et al. 2017. Mol Ther. 10.1016/j.ymthe.2017.01.020. PubMed
  105. Onai N, et al. 2020. Int J Mol Sci. :22. PubMed
  106. Celik H, et al. 2018. Cancer Cell. 34:741. PubMed
  107. Kang YH, et al. 2019. Nat Commun. 10:912. PubMed
  108. Castañeda–Zegarra S, et al. 2019. Biomolecules. 0.929166667. PubMed
  109. Casanova-Acebes M, et al. 2018. J Exp Med. 215:2778. PubMed
  110. Omatsu Y, et al. 2022. Nat Commun. 13:2654. PubMed
  111. Jing Y, et al. 2021. Front Immunol. 12:651860. PubMed
  112. Bergquist M, et al. 2014. J Infect. 69:113. PubMed
  113. McCauley ME, et al. 2020. Nature. 585(7823):96-101. PubMed
  114. Ulaganathan VK, et al. 2020. Sci Rep. 10:8453. PubMed
  115. Düking T, et al. 2022. Sci Adv. 8:eabo7639. PubMed
  116. Shaikh H, et al. 2021. Front Immunol. 12:689896. PubMed
  117. Bajaña S, et al. 2022. iScience. 25:103732. PubMed
  118. Koide S, et al. 2022. iScience. 25:103603. PubMed
  119. Zhu E, et al. 2014. J Immunol. 192:5599. PubMed
  120. Yang X, et al. 2021. Bioact Mater. 3150:6. PubMed
  121. Rousso-Noori L, et al. 2021. Nat Commun. 12:3615. PubMed
  122. Zhou S, et al. 2021. Adv Funct Mater. 31:. PubMed
  123. Sato Y, et al. 2022. Front Mol Biosci. 9:1040237. PubMed
  124. Bonavita E, et al. 2020. Immunity. 1215:53. PubMed
  125. Pelly V, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.4. PubMed
  126. Arensman MD, et al. 2020. Oncoimmunology. 9:1800162. PubMed
  127. Parks CA, et al. 2019. Proc Natl Acad Sci U S A. 116:3136. PubMed
  128. Chryplewicz A, et al. 2022. Cancer Cell. 40:1111. PubMed
  129. Stephens HM, et al. 2022. Methods Mol Biol. 2478:727. PubMed
  130. Sun Y, et al. 2022. iScience. 25:104846. PubMed
  131. Xu G, et al. 2015. Free Radic Biol Med. 87: 15-25. PubMed
  132. Cruz F, et al. 2016. Stem Cells Trans Med. 5: 488-499. PubMed
  133. Huang X, et al. 2021. Immunity. . PubMed
  134. Timilshina M, et al. 2020. Cell Reports. 27(10):2948-2961.e7.. PubMed
  135. Radulovic V, et al. 2020. Cell Reports. 27(10):2826-2836.e5.. PubMed
  136. Liu Z, et al. 2020. STAR Protoc. 1:100029. PubMed
  137. Dourcy M, et al. 2020. Mucosal Immunol. 13:799. PubMed
  138. Harvey RE et al. 2017. Endocrinology. 158(7):2179-2189 . PubMed
  139. Campisi L, et al. 2016. Nat Immunol. 10.1038/ni.3512. PubMed
  140. Yu YR, et al. 2020. STAR Protocols. 11(3):e0150606. PubMed
  141. Omenetti S, et al. 2019. Immunity. 51:77. PubMed
  142. Tong Y, et al. 2018. EBioMedicine. 39:132. PubMed
  143. Shi GN, et al. 2021. Ther Adv Med Oncol. 13:1758835920987056. PubMed
RRID
AB_312676 (BioLegend Cat. No. 100311)
AB_312676 (BioLegend Cat. No. 100312)

Antigen Details

Structure
Ig superfamily, forms CD3/TCR complex with CD3δ, γ and ζ subunits and TCR (α/β and γ/δ), 20 kD
Distribution

Thymocytes (differentiation dependent), mature T cells, NK-T cells

Function
TCR signal transduction, T cell activation, antigen recognition
Ligand/Receptor
Peptide antigen/MHC-complex
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, TCRs
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263.

Gene ID
12501 View all products for this Gene ID
UniProt
View information about CD3epsilon on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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