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FITC anti-mouse CD3ε Antibody

Pricing & Availability
Clone
145-2C11 (See other available formats)
Regulatory Status
RUO
Other Names
CD3ε, T3, CD3
Isotype
Armenian Hamster IgG
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Product Citations
publications
145-2C11_FITC_021606
C57BL/6 mouse splenocytes were stained with CD3e (clone 145-2C11) FITC and CD127 (clone SB/199) PE.
  • 145-2C11_FITC_021606
    C57BL/6 mouse splenocytes were stained with CD3e (clone 145-2C11) FITC and CD127 (clone SB/199) PE.
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100305 50 µg $30
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100306 500 µg $99
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Description

CD3ε is a 20 kD transmembrane protein, also known as CD3 or T3. It is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3ε forms a TCR complex by associating with the CD3δ, γ and ζ chains, as well as the TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
H-2Kb-specific mouse cytotoxic T lymphocyte clone BM10-37
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Clone 145-2C11 is useful for in vitro blocking of target-specific CTL-mediated cell lysis1, as well as T cell activation assays, inducing proliferation and cytokine production1,2,7,12,16. It also induces apoptosis in immature thymocytes32,  and in vivo T cell depletion8-10. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation1, immunohistochemical staining14,15 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, Western blotting4, complement-mediated cytotoxicity6, in vitro and in vivo stimulation of T cells1,2,7,12,16, immunofluorescent staining5, and in vivo T cell depletion8-10. The 145-2C11 antibody has been reported to block the binding of 17A2 antibody to CD3 epsilon-specific T cells11. Clone 145-2C11 is not recommended for formalin-fixed paraffin embedded sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 100314). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 100340) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Leo O, et al. 1987. P. Natl. Acad. Sci. USA 84:1374. (IP, Activ, Block)
  2. Kruisbeek AM, et al. 1991. In Current Protocols in Immunology. 3.12.1. (Activ)
  3. Duke RC, et al. 1995. Current Protocols in Immunology. 3.17.1.
  4. Salvadori S, et al. 1994. J. Immunol. 153:5176. (WB)
  5. Payer E, et al. 1991. J. Immunol. 146:2536. (IF)
  6. Jacobs H, et al. 1994. Eur. J. Immunol. 24:934. (CMCD)
  7. Vossen ACTM, et al. 1995. Eur. J. Immunol. 25:1492. (Activ)
  8. Henrickson M, et al. 1995. Transplantation 60:828. (Deplete)
  9. Kinnaert P, et al. 1996. Transpl. Int. 9:386. (Deplete)
  10. Han WR, et al. 1999. Transpl. Immunol. 7:207. (Deplete)
  11. Miescher GC, et al. 1989. Immunol. Lett. 23:113. (Block)
  12. Terrazas LI, et al. 2005. Intl. J. Parasitology. 35:1349. (Activ)
  13. Ko SY, et al. 2005. J. Immunol. 175:3309.
  14. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC-F)
  15. Tilley SL, et al. 2007. J. Immunol. 178:3208. (IHC-F)
  16. Wang W, et al. 2007. J. Immunol. 178:4885. (Activ)
  17. Xiao S, et al. 2007. J. Exp. Med. 204:1691.
  18. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed.
  19. Curtsinger JM, et al.2005. J. Immunol. 175:4392. PubMed
  20. Guo Y, et al. 2008. Blood 112:480. PubMed
  21. Kenna TJ, et al. 2008. Blood 111:2091.
  22. Perchonock CE, et al. 2007. J. Immunol. 179:1768. PubMed
  23. Perchonock GE, et al. 2006. Mol. Cell. Biol. 26:6005. PubMed
  24. Kanaya T, et al. 2008. Am. J. Physiol. Gastrointest. Liver Physiol. 295:G273. PubMed
  25. de Koning BA, et al. 2006. Int. Immunol. 18:941. PubMed
  26. Schulteis RD, et al. 2008. Blood 295:G273. PubMed
  27. Qi Q, et al. 2009. Blood 114:564. PubMed
  28. Helmersson S, et al. 2013. Am J Pathol. 9440:123. Pubmed
  29. Wu S, et al. 2014. Clin Vaccine Immunol. 21:156. PubMed
  30. Yan J, et al. 2014. Vaccine. 32:2833. PubMed
  31. Guiterrez DA, et al. 2014. Diaebetes. 63:3827. PubMed
  32. Shi YF, et al. 1991. J Immunol. 146:3340. (Apop)
Product Citations
  1. Bertrand F, et al. 2017. Nat Commun. 8:2256. PubMed
  2. Katsumura KR, et al. 2018. Proc Natl Acad Sci U S A. 115:E10109. PubMed
  3. Hou X, et al. 2020. Cell Reports. 28(1):172-189.e7.. PubMed
  4. Tomida S, et al. 2019. Sci Rep. 9:10751. PubMed
  5. Chei S, et al. 2020. Front Nutr. 7:583186. PubMed
  6. Russler-Germain EV, et al. 2021. Immunity. 54:2547. PubMed
  7. Li DD, et al. 2022. J Cell Mol Med. 26:2438. PubMed
  8. Jou E, et al. 2022. Sci Immunol. 7:eabn0175. PubMed
  9. Zhao X, et al. 2022. STAR Protoc. 3:101859. PubMed
  10. Yang L, et al. 2023. Acta Pharmacol Sin. :. PubMed
  11. Lu T, et al. 2023. Cancer Immunol Immunother. :. PubMed
  12. Smith LA, et al. 2023. Front Oncol. 12:1031174. PubMed
  13. Murakata Y, et al. 2023. Front Cardiovasc Med. 9:1075358. PubMed
  14. Jerome JR, et al. 2023. Int J Mol Sci. 24: . PubMed
  15. Drijvers JM, et al. 2021. Cancer Immunol Res. 9:184. PubMed
  16. Glorieux C, et al. 2022. J Adv Res. 40:109. PubMed
  17. Wu R, et al. 2022. Nat Immunol. 23:1536. PubMed
  18. Lu C, et al. 2023. Antiviral Res. 212:105556. PubMed
  19. Wang JX, et al. 2023. JCI Insight. 8:. PubMed
  20. Wu Y, et al. 2023. Nat Cancer. 4:382. PubMed
  21. Brunner SM, et al. 2023. Int J Mol Sci. 24:. PubMed
  22. Senatus L, et al. 2023. Commun Biol. 6:280. PubMed
  23. Stitzlein LM, et al. 2023. Front Neurol. 14:1112207. PubMed
  24. van Os BW, et al. 2023. Front Cardiovasc Med. 10:1171764. PubMed
  25. Ye S, et al. 2023. Front Oncol. 13:1202750. PubMed
  26. Zhou J, et al. 2021. Int J Med Sci. 18:3516. PubMed
  27. Lentucci C, et al. 2017. J Biol Chem. 292:2754-2772. PubMed
  28. Schulteis R, et al. 2008. Blood. 112:4905. PubMed
  29. Wang L, et al. 2019. Cell Rep. 29:1848. PubMed
  30. Groza D, et al. 2018. Oncoimmunology. 7:e1424676. PubMed
  31. Koning B, et al. 2006. Int Immunol. 1.403472222. PubMed
  32. Fornari T, et al. 2015. PLoS One. 10: 0142688. PubMed
  33. Hewitson JP, et al. 2020. J Immunol. 204:2949. PubMed
  34. Johnson KD, et al. 2022. Blood Adv. 6:1464. PubMed
  35. Waide ML, et al. 2020. Cell Rep. 33:108503. PubMed
  36. Kremenovic M, et al. 2022. J Immunother Cancer. 10:. PubMed
  37. Tian H, et al. 2022. Cancer Sci. 113:875. PubMed
  38. Lou F, et al. 2020. STAR Protoc. 1:100115. PubMed
  39. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  40. Gruber T, et al. 2020. JCI Insight. 5:00. PubMed
  41. Allegrezza M, et al. 2016. Cancer Res . 76: 6253 - 6265. PubMed
  42. Yang W, et al. 2020. Nat Commun. 3.553472222. PubMed
  43. Isoda T et al. 2017. Cell. 171(1):103-119 . PubMed
  44. Mann M, et al. 2018. Cell Rep. 25:2992. PubMed
  45. Hung PJ, et al. 2018. Mol Cell. 71:332. PubMed
  46. Patnode M, et al. 2013. Glycobiology. 23:381. PubMed
  47. Bartleson JM, et al. 2020. Nat Immunol. 1384:21. PubMed
  48. Hoffman D, et al. 2021. Immunity. 54:2712. PubMed
  49. Uchida A, et al. 2021. J Inflamm Res. 14:3089. PubMed
  50. Sato Y, et al. 2021. J Clin Invest. Online ahead of print. PubMed
  51. Yi M, et al. 2021. J Hematol Oncol. 14:27. PubMed
  52. Zhang X, et al. 2020. J Immunol. 205:1743. PubMed
  53. Peng L, et al. 2020. Transl Lung Cancer Res. 0.892361111. PubMed
  54. Best SA, et al. 2018. Cell Metab. 27:935. PubMed
  55. Emgård J, et al. 2018. Immunity. 143:419. PubMed
  56. Lin JR et al. 2018. eLife. 7 pii: e31657. PubMed
  57. Shimokawa C et al. 2017. Immunity. 46(5):863-874 . PubMed
  58. Qi X, et al. 2019. Nat Med. 25:1225. PubMed
  59. Collin R, et al. 2014. J Immunol. 193:3503. PubMed
  60. Yin Q, et al. 2015. PLoS One. 10: 0137808. PubMed
  61. Ma Y, et al. 2015. J Immunol. 195: 3769 - 3780. PubMed
  62. Matsumura K, et al. 2016. J Immunol. 197: 3233 - 3244. PubMed
  63. Pereira JL, et al. 2021. Transl Oncol. 14:101125. PubMed
  64. Li H, et al. 2022. Theranostics. 12:6422. PubMed
  65. Jeong SH, et al. 2021. Nat Commun. 12:4405. PubMed
  66. Huang Y, et al. 2020. FASEB J. 34:1768. PubMed
  67. Kawakami R, et al. 2021. Immunity. 54(5):947-961.e8. PubMed
  68. Burrack K, et al. 2015. PLoS Pathog. 11: e1005191. PubMed
  69. Skeate JG, et al. 2018. PLoS One. 13:e0191311. PubMed
  70. He L, et al. 2019. iScience. 0.790972222. PubMed
  71. Cheng HW, et al. 2019. Nat Commun. 10:1739. PubMed
  72. Cong J et al. 2018. Cell metabolism. 28(2):243-255 . PubMed
  73. Knolle MD et al. 2018. Frontiers in immunology. 1.925 . PubMed
  74. Doonan J, et al. 2019. Nat Commun. 10:1554. PubMed
  75. Srivastava S, et al. 2019. Cancer Cell. 35:489. PubMed
  76. Miki Y, et al. 2016. J Biol Chem. 291: 15588 - 15601. PubMed
  77. Tang CC, et al. 2021. eLife. 10:00. PubMed
  78. Gong Y, et al. 2020. Cell Metabolism. 33(1):51-64.e9. PubMed
  79. He Y, et al. 2021. Cell Metabolism. 33(5):988-1000.e7. PubMed
  80. Souza COS, et al. 2021. iScience. 24(6):102548. PubMed
  81. Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
  82. Russler-Germain EV, et al. 2021. Elife. 10:. PubMed
  83. Pardy RD, et al. 2021. Nat Commun. 12:4051. PubMed
  84. Yang H, et al. 2022. Nutrients. 14:. PubMed
  85. Kondratieva T, et al. 2020. PLoS One. 15:e0239668. PubMed
  86. Harnett MM, et al. 2022. Front Immunol. 13:953053. PubMed
  87. Zeng Q, et al. 2022. Front Immunol. 13:740805. PubMed
  88. Stotesbury C, et al. 2020. J Immunol. 204:1582. PubMed
  89. Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed
  90. Yi X, et al. 2020. Sci Adv. 6:eaba3546. PubMed
  91. Burrack K, et al. 2015. J Immunol. 194:678. PubMed
  92. Siamishi I, et al. 2020. Cell Reports. 31(11):107756. PubMed
  93. Xiang W, et al. 2018. Nat Commun. 9:2574. PubMed
  94. Kacherovsky N, et al. 2019. Nat Biomed Eng. 0.66875. PubMed
  95. Wanner-Seleznik GM, et al. 2020. Immunohorizons. 0.644444444. PubMed
  96. Deerhake ME, et al. 2021. Immunity. 54(3):484-498.e8. PubMed
  97. Kim SH, et al. 2021. Cell Reports. 35(2):108995. PubMed
  98. Murakami K, et al. 2021. Cell Reports. 34(1):108579. PubMed
  99. Ni J, et al. 2020. Immunity. 52(6):1075-1087.e8. PubMed
  100. Bian G, et al. 2020. British Journal of Pharmacology. 178(3):636-653. PubMed
  101. Galle-Treger L, et al. 2016. Nat Commun. 7:13202. PubMed
  102. Sendler M, et al. 2020. Gastroenterology. 158:253. PubMed
  103. Denny JE, et al. 2019. Sci Rep. 2.786111111. PubMed
  104. Kim MY, et al. 2017. Plant Biotechnol J. 1.729166667. PubMed
  105. Xu M, et al. 2013. J Immunol. 190:5436. PubMed
  106. Huang J, et al. 2014. J Immunol. 192:1972. PubMed
  107. Xu Q, et al. 2021. Theranostics. 11:1937. PubMed
  108. Zhang P, et al. 2022. Nat Commun. 13:1588. PubMed
  109. Vacca F, et al. 2020. eLife. 9:e54017.. PubMed
  110. Labiano S, et al. 2017. Oncoimmunology. 6:e1283468. PubMed
  111. Engblom C, et al. 2017. Science. 358:6367. PubMed
  112. Baomei Wang et al. 2019. Cell reports. 26(6):1614-1626 . PubMed
  113. Kang YH, et al. 2019. Nat Commun. 10:912. PubMed
  114. Eisemann T, et al. 2019. Front Oncol. 9:187. PubMed
  115. Yue W, et al. 2019. Nat Commun. 10:2025. PubMed
  116. Oliveira AC et al. 2017. eLife. 6 pii: e30883. PubMed
  117. Lind L, et al. 2021. J Neurovirol. 27:145. PubMed
  118. Agrawal M, et al. 2022. Blood. 140:1094. PubMed
  119. Zhao X, et al. 2022. iScience. 25:104690. PubMed
  120. Soria-Castro R, et al. 2020. Sci Rep. 10:17802. PubMed
  121. Sánchez-del-Campo L, et al. 2021. J Exp Clin Cancer Res. 40:117. PubMed
  122. Casselli T, et al. 2021. PLoS Pathog. 17:e1009256. PubMed
  123. Okada R, et al. 2021. EBioMedicine. 67:103345. PubMed
  124. Luo L, et al. 2021. J Neuroinflammation. 18:27. PubMed
  125. Damgaard RB et al. 2016. Cell. 166(5):1215-1230 . PubMed
  126. Baglaenko Y, et al. 2016. PLoS One. 11: 0150515. PubMed
  127. Wang J, et al. 2019. Aging (Albany NY). 11:3463. PubMed
  128. Lin JC, et al. 2019. Nat Commun. 10:1057. PubMed
  129. Liu Y, et al. 2018. Cancer Cell. 33:480. PubMed
  130. Sparber F, et al. 2019. Cell Host Microbe. 25:389. PubMed
  131. Zegarra‐Ruiz DF et al. 2018. Cell host & microbe. 25(1):113-127 . PubMed
  132. Okuyama Y, et al. 2020. FASEB J. 540:34. PubMed
  133. La Salvia S, et al. 2020. Am J Physiol Renal Physiol. 319:F868. PubMed
  134. Fitzpatrick Z, et al. 2020. Nature. 587:472. PubMed
  135. Oh J, et al. 2021. Clin Cancer Res. 27:4781. PubMed
  136. Harding CL, et al. 2020. Front Cell Infect Microbiol. 10:328. PubMed
  137. Xiao Z, et al. 2022. Mater Today Bio. 15:100297. PubMed
  138. Ma C, et al. 2021. Mol Metab. 47:101170. PubMed
  139. Srivastava S, et al. 2020. Cancer Cell. 39(2):193-208.e10. PubMed
  140. Sheng J, et al. 2021. eLife. 10:00. PubMed
  141. Nakagawa Y, et al. 2015. Immunol Lett. 167: 72-86. PubMed
  142. Ikeda T, et al. 2014. PLoS One. 9:115198. PubMed
  143. Burton OT, et al. 2018. Clin Exp Allergy. 48:288. PubMed
  144. Tippimanchai DD, et al. 2018. Oncoimmunology. 7:e1438105. PubMed
  145. Schofield L, et al. 2017. BMC Med. 10.1186/s12916-017-0883-8. PubMed
  146. Hilt ZT, et al. 2019. JCI Insight. 4:5. PubMed
  147. Krotova K, et al. 2019. Mol Ther Oncolytics. 15:166. PubMed
  148. Lai NY, et al. 2020. Cell. 180:33:00. PubMed
  149. Kanaya T, et al. 2008. Am J Physiol Gastrointest Liver Physiol. 295:273. PubMed
  150. Zhang S, et al. 2016. PLoS Pathog. 12: 1005617. PubMed
  151. L M, et al. 2016. Brain. 139: 1939-1957. PubMed
  152. Tian T, et al. 2020. Cancer Immunol Res. 660:8. PubMed
  153. Zhou X, et al. 2021. Nutr Metab (Lond). 18:64. PubMed
  154. Guo Y, et al. 2021. Nat Immunol. 22:746. PubMed
  155. Rivera CA, et al. 2022. Immunity. 55:129. PubMed
  156. Awida Z, et al. 2021. Int J Mol Sci. 23:. PubMed
  157. Amatruda M, et al. 2022. Brain Commun. 4:fcac044. PubMed
  158. Aggarwal N, et al. 2021. Cell Rep. 37:110170. PubMed
RRID
AB_312670 (BioLegend Cat. No. 100305)
AB_312670 (BioLegend Cat. No. 100306)

Antigen Details

Structure
Ig superfamily, forms CD3/TCR complex with CD3δ, γ and ζ subunits and TCR (α/β and γ/δ), 20 kD
Distribution

Thymocytes (differentiation dependent), mature T cells, NK-T cells

Function
TCR signal transduction, T cell activation, antigen recognition
Ligand/Receptor
Peptide antigen/MHC-complex
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, TCRs
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263.

Gene ID
12501 View all products for this Gene ID
UniProt
View information about CD3epsilon on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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