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APC anti-mouse CD4 Antibody

Pricing & Availability
Clone
RM4-5 (See other available formats)
Regulatory Status
RUO
Other Names
L3T4, T4
Isotype
Rat IgG2a, κ
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Product Citations
publications
RM4-5_APC_030206
C57BL/6 mouse splenocytes stained with CD4 (clone RM4-5) APC (filled histogram) or rat IgG2a, κ APC isotype control (open histogram).
  • RM4-5_APC_030206
    C57BL/6 mouse splenocytes stained with CD4 (clone RM4-5) APC (filled histogram) or rat IgG2a, κ APC isotype control (open histogram).
Compare all formats See APC spectral data
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100515 25 µg $33
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100516 100 µg $105
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Description

CD4 is a 55 kD protein also known as L3T4 or T4. It is a member of the Ig superfamily, primarily expressed on most thymocytes and a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a co-receptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosine kinase lck.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
BALB/c mouse thymocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The CD4 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

The RM4-5 antibody blocks the binding of GK1.5 antibody and H129.19 antibody to CD4+ T cells, but not RM4-4 antibody. Additional reported applications (for the relevant formats) include: blocking of ligand binding, in vivo depletion of CD4+ cells1, and immunohistochemistry of acetone-fixed frozen tissue sections2,3,11 and paraffin-embedded sections11. Clone RM4-5 is not recommended for immunohistochemistry of formalin-fixed paraffin sections. Instead, acetone frozen or zinc-fixed paraffin sections are recommended. The Ultra-LEAF™ Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 100575 and 100576).

Application References

(PubMed link indicates BioLegend citation)
  1. Kruisbeek AM. 1991. In Curr. Protocols Immunol. pp. 4.1.1-4.1.5. (Block, Deplete)
  2. Nitta H, et al. 1997. Cell Vision 4:73. (IHC)
  3. Fan WY, et al. 2001. Exp. Biol. Med. 226:1045.
  4. Muraille E, et al. 2003. Infect. Immun. 71:2704. (IHC)
  5. León-Ponte M, et al. 2007. Blood 109:3139. (FC)
  6. Bourdeau A, et al. 2007. Blood doi:10.1182/blood-2006-08-044370. (FC)
  7. Matsumoto M, et al. 2007.J. Immunol.178:2499. PubMed
  8. Shigeta A, et al. 2008. Blood 112:4915. PubMed
  9. Zaborsky N, et al. 2010. J. Immunol. 184:725. PubMed
  10. Rodrigues-Manzanet R, et al. 2010. P. Natl Acad Sci USA 107:8706. PubMed
  11. Whiteland JL, et al. 1995. J. Histochem. Cytochem. 43:313. (IHC)
Product Citations
  1. Soon MSF, et al. 2020. Nat Immunol. 1.984027778. PubMed
  2. Cosgrove J, et al. 2020. Nat Commun. 3.011805556. PubMed
  3. Ying W, et al. 2019. Cell Metab. 29:457. PubMed
  4. Schnell A, et al. 2021. Cell. 184:6281. PubMed
  5. Casado-García A, et al. 2022. Cancer Res. 82:1098. PubMed
  6. Liu H, et al. 2022. Cancer Immunol Res. 10:1028. PubMed
  7. Wang W, et al. 2022. Cell Rep. 41:111582. PubMed
  8. Zheng D, et al. 2023. Bioact Mater. 22:567. PubMed
  9. Li J, et al. 2022. Front Immunol. 13:1043484. PubMed
  10. Gon Y, et al. 2023. PLoS One. 18:e0279389. PubMed
  11. Azevedo PO, et al. 2023. NPJ Vaccines. 8:15. PubMed
  12. Issa N, et al. 2023. Leukemia. :. PubMed
  13. Zhang X, et al. 2023. Front Immunol. 14:1116749. PubMed
  14. van Os BW, et al. 2023. Eur Heart J Open. 3:oead013. PubMed
  15. van Os BW, et al. 2023. Front Cardiovasc Med. 10:1171764. PubMed
  16. McNamara HA, et al. 2020. Cell Host Microbe. 572:28. PubMed
  17. Hirako I, et al. 2016. Nat Commun. 7:13277. PubMed
  18. Zhang LJ, et al. 2019. Immunity. 50:121. PubMed
  19. Suzuki K, et al. 2019. Cell Mol Gastroenterol Hepatol. 8:119. PubMed
  20. Lee S, et al. 2021. JCI Insight. 6:. PubMed
  21. Yu Y, et al. 2022. iScience. 25:105004. PubMed
  22. Fisher E, et al. 2021. Front Immunol. 11:602254. PubMed
  23. Xia S, et al. 2014. J Leukoc Biol. 95:733. PubMed
  24. Lee J, et al. 2020. Gut Microbes. 1:. PubMed
  25. Misumi I et al. 2019. Cell Rep. 27(5):1387-1396 . PubMed
  26. Matsumoto M, et al. 2007. J Immunol . 178:2499. PubMed
  27. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  28. Rodriguez-Manzanet R, et al. 2010. Proc Natl Acad Sci U S A. 107:8706. PubMed
  29. Zhao B, et al. 2020. Nat Commun. 11:908. PubMed
  30. Bhattacharjee S, et al. 2019. Cell Rep. 28:231. PubMed
  31. Pokrovskii M, et al. 2020. EMBO J. 39:e104159. PubMed
  32. Webster HC, et al. 2020. J Immunol Methods. 112702:477. PubMed
  33. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  34. Misumi I et al. 2019. Cell Rep. 27(2):514-524 . PubMed
  35. LaFleur MW, et al. 2019. Nat Immunol. 20:1335. PubMed
  36. Ayasoufi K, et al. 2020. Brain. 3629:143. PubMed
  37. Perner C, et al. 2020. Immunity. 53(5):1063-1077.e7. PubMed
  38. Huang B, et al. 2020. Cell. 179(5):1160-1176.e24.. PubMed
  39. Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed
  40. Cao W, et al. 2017. Immunity. 47:1182. PubMed
  41. Li Y, et al. 2020. Nat Commun. 11:2781. PubMed
  42. Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed
  43. Shigeta A, et al. 2008. Blood. 112:4915. PubMed
  44. van der Lelie D, et al. 2021. Nat Commun. 12:3105. PubMed
  45. Kang S, et al. 2021. Bio Protoc. 11:e4029. PubMed
  46. Nakajima A, et al. 2014. PLoS One. 9:105904. PubMed
  47. Deng Z, et al. 2017. Oncogene. 36:639. PubMed
  48. Miska J et al. 2019. Cell reports. 27(1):226-237 . PubMed
  49. Park S, et al. 2013. J Immunol. 190:3480. PubMed
  50. Mencarelli A, et al. 2016. Sci Rep. 6:30802. PubMed
  51. Sheng W et al. 2018. Cell. 174(3):549-563 . PubMed
  52. LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed
  53. Anger-Góra N, et al. 2021. Oncol Lett. 22:582. PubMed
  54. Koikawa K, et al. 2021. Cell. 184:4753. PubMed
  55. Rovira-Clavé X, et al. 2016. J Leukoc Biol. 99: 143 - 152. PubMed
  56. Tsumiyama K, et al. 2013. J Immunol. 191:91. PubMed
  57. Chen Z, et al. 2019. J Exp Med. 216:152. PubMed
  58. Lin YW, et al. 2022. Mol Med Rep. 26:. PubMed
  59. Moutuou MM, et al. 2020. Methods Mol Biol. 2111:267. PubMed
  60. Lin J, et al. 2021. Nat Commun. 12:5321. PubMed
  61. Huang J, et al. 2014. J Immunol. 192:1972. PubMed
  62. Biton J, et al. 2016. J Immunol. 197: 1708 - 1719. PubMed
  63. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
  64. Wu S, et al. 2021. Nat Cancer. 2:189. PubMed
  65. Nahrendorf W, et al. 2021. eLife. 10:00. PubMed
  66. Yuan X, et al. 2017. Elife. 6:e29540. PubMed
  67. Feng D, et al. 2022. Nat Commun. 13:5554. PubMed
  68. Izumi G, et al. 2021. Nat Commun. 12:5029. PubMed
  69. Robertson TF, et al. 2021. J Cell Biol. 220:. PubMed
  70. Xiao u, et al. 2015. J Immunol. 195: 4218 - 4227. PubMed
  71. Martina M, et al. 2016. J Am Soc Nephrol. 27: 1113-1123. PubMed
  72. Wang T et al. 2018. Immunity. 49(3):504-514 . PubMed
  73. Urata S, et al. 2018. PLoS Pathog. 14:e1007172. PubMed
  74. Zaborsky N, et al. 2010. J Immunol. 184:725. PubMed
  75. Roy S, et al. 2021. Nat Commun. 12:3182. PubMed
  76. Liu J, et al. 2021. Front Immunol. 12:733808. PubMed
  77. Ene CI, et al. 2020. Neuro Oncol. 639:22. PubMed
  78. Lee Y, et al. 2012. Nat Immunol. 13:991. PubMed
  79. Ulaganathan VK, et al. 2020. Sci Rep. 10:8453. PubMed
  80. van den Berk P, et al. 2020. Cell Rep. 33:108533. PubMed
  81. Schaffert S, et al. 2015. J Immunol. 195: 1470-1479. PubMed
  82. Hoechst B, et al. 2016. J Immunol. 195:1517-1523. PubMed
  83. Kramer K, et al. 2017. Mol Ther. 25(1):62-70. PubMed
  84. Takahashi T, et al. 2017. J Exp Med. 10.1084/jem.20160247. PubMed
  85. Yin X, et al. 2020. Cell Rep. 33:108278. PubMed
  86. Denzin LK et al. 2017. Immunity. 47(2):310-322 . PubMed
  87. Hartmann W, et al. 2020. Cell Reports. 29(8):2243-2256.e4.. PubMed
  88. Kohn M, et al. 2021. Front Immunol. 626627:12. PubMed
  89. Guo X, et al. 2020. J Cell Mol Med. . PubMed
  90. Pelly V, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.4. PubMed
  91. Berry G, Waldner H 2013. J Vis Exp. 75: 50389. PubMed
  92. St Clair JR, et al. 2018. Nat Commun. 9:1742. PubMed
  93. Sudo K, et al. 2021. Stem Cell Res. 56:102531. PubMed
  94. Skirecki T, et al. 2020. JCI Insight. 5:. PubMed
  95. Li C, et al. 2014. J Immunol. 192:1425. PubMed
  96. Zaiss M, et al. 2015. Immunity. 43: 998-1010. PubMed
  97. Jaeger N, et al. 2020. Cell Rep. 33:108331. PubMed
  98. Arima Y et al. 2017. eLife. 6 pii: e25517. PubMed
  99. García–Rodríguez S, et al. 2018. Sci Rep. 2.664583333. PubMed
  100. Wen X, et al. 2021. World J Gastroenterol. 27:2834. PubMed
  101. Parigger T, et al. 2021. Int J Mol Sci. 22:. PubMed
  102. Dourcy M, et al. 2020. Mucosal Immunol. 13:799. PubMed
  103. Palaniyandi S, et al. 2013. PLoS One. 8:61841. PubMed
  104. Pastor Arroyo EM, et al. 2022. Clin Sci (Lond). 136:557. PubMed
  105. Liu Y, et al. 2021. Nat Commun. 12:6831. PubMed
RRID
AB_312718 (BioLegend Cat. No. 100515)
AB_312718 (BioLegend Cat. No. 100516)

Antigen Details

Structure
Ig superfamily, 55 kD
Distribution

Majority of thymocytes, T cell subset

Function
TCR co-receptor, T cell activation
Ligand/Receptor
MHC class II molecule
Cell Type
Dendritic cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Bierer BE, et al. 1989. Annu. Rev. Immunol. 7:579.
3. Janeway CA. 1992. Annu. Rev. Immunol. 10:645.

Gene ID
12504 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

Related FAQs

I am unable to see expression of T cell markers such as CD3 and CD4 post activation.
TCR-CD3 complexes on the T-lymphocyte surface are rapidly downregulated upon activation with peptide-MHC complex, superantigen or cross-linking with anti-TCR or anti-CD3 antibodies. PMA/Ionomycin treatment has been shown to downregulate surface CD4 expression. Receptor downregulation is a common biological phenomenon and so make sure that your stimulation treatment is not causing it in your sample type.
Go To Top Version: 3    Revision Date: 01/29/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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