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APC anti-mouse Ly-6C Antibody

Pricing & Availability
Clone
HK1.4 (See other available formats)
Regulatory Status
RUO
Other Names
Lymphocyte antigen 6 complex, locus C
Isotype
Rat IgG2c, κ
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Product Citations
publications
HK1dot4_APC_082509
C57BL/6 bone marrow cells stained with HK1.4 APC
  • HK1dot4_APC_082509
    C57BL/6 bone marrow cells stained with HK1.4 APC
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128015 25 µg $116
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128016 100 µg $286
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Description

Most hematopoietic cells express one or more members of Ly-6 family. The expression of Ly-6 varies with development stage and activation. Ly-6C is a 14-17 kD GPI-linked surface protein expressed on mouse monocyte/macrophage cells, endothelial cells, neutrophils, and some T cell subsets. Ly-6C is reported to be an indicator of memory CD8+ T cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
L3 cloned CTL cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Clone HK1.4 does not block the binding of clone RB6-8C58.

Additional reported applications (for relevant formats of this clone) include: in vitro activation of T cells1-3 and immunohistochemistry of frozen sections4.

Application References

(PubMed link indicates BioLegend citation)
  1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819. (Activ)
  2. Herold KC, et al. 1990. Diabetes 39:815. (Activ)
  3. Havran WL, et al. 1988. J. Immunol. 140:1034 (Activ)
  4. Flanagan K, et al. 2008. J. Immunol. 180:3874. (IHC)
  5. Makaroff LE, et al. 2009. P. Natl. Acad. Sci. USA 106:4799. (FC)
  6. Zuber J, et al. 2009. Genes Dev. 23:877. (FC) PubMed
  7. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  8. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  9. Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
Product Citations
  1. Guo H, et al. 2020. Curr Protoc Immunol. 131:e107. PubMed
  2. Pierson M, et al. 2021. Curr Protoc. 1:e53. PubMed
  3. Alam A, et al. 2022. Cancer Cell. 40:153. PubMed
  4. Bettke JA, et al. 2022. Infect Immun. 90:e0007022. PubMed
  5. Dolfi B, et al. 2022. Cell Rep. 39:110949. PubMed
  6. Blanco LP, et al. 2022. Arthritis Rheumatol. 74:1971. PubMed
  7. Poh AR, et al. 2022. Cell Rep. 41:111479. PubMed
  8. Gong F, et al. 2023. Adv Healthc Mater. 12:e2201771. PubMed
  9. Papaxenopoulou LA, et al. 2022. iScience. 25:105522. PubMed
  10. Urata S, et al. 2023. Microbiol Immunol. 67:105. PubMed
  11. He Y, et al. 2023. Nat Commun. 14:691. PubMed
  12. Drummer C, et al. 2023. Front Immunol. 14:1113883. PubMed
  13. Tebartz C, et al. 2015. J Immunol. 194:1100. PubMed
  14. D'Alessandro G, et al. 2020. Eur J Immunol. 50:705. PubMed
  15. MohanKumar K, et al. 2019. Nat Commun. 10:3494. PubMed
  16. Hamdan F, et al. 2023. Mol Ther Oncolytics. 28:264. PubMed
  17. Saha S, et al. 2023. Sci Rep. 13:4609. PubMed
  18. Fukuzaki Y, et al. 2023. iScience. 26:106340. PubMed
  19. Cozzolino M, et al. 2023. Int J Mol Sci. 24:. PubMed
  20. Yang QC, et al. 2023. iScience. 26:106916. PubMed
  21. Tan L, et al. 2022. Biochem Biophys Rep. 32:101351. PubMed
  22. Li X, et al. 2022. Nat Commun. 13:2794. PubMed
  23. Aggen DH, et al. 2020. Clin Cancer Res. . PubMed
  24. Kim JS, et al. 2020. Immunity. 54(1):176-190.e7. PubMed
  25. Nocera D, et al. 2016. J Immunol. 196: 2860 - 2869. PubMed
  26. Rosenheinrich M, et al. 2015. PLoS One. 10: 0136290. PubMed
  27. Borggrewe M, et al. 2018. Glia. 66:2645. PubMed
  28. Iwamoto H et al. 2018. Cell metabolism. 28(1):104-117 . PubMed
  29. Jahan S, et al. 2022. J Biol Chem. :102386. PubMed
  30. Price P, et al. 2014. J Virol. 88:10840. PubMed
  31. Zasłona Z, et al. 2014. J Immunol. 193:4245. PubMed
  32. Su N, Xiong Y 2016. Sci Rep. 6:22658. PubMed
  33. Kurelac I, et al. 2019. Nat Commun. 10:903. PubMed
  34. Shu T, et al. 2022. Eur Respir J. Online ahead of print. PubMed
  35. Cheng G, et al. 2021. iScience. 24:102673. PubMed
  36. Pingili AK, et al. 2021. Cell Reports. 35(12):109285. PubMed
  37. Earnest JT, et al. 2021. Cell Reports. 35(1):108962. PubMed
  38. Liang W, et al. 2020. Nat Commun. 4.563888889. PubMed
  39. Biffi G, et al. 2018. Cancer Discov. 2:282. PubMed
  40. Zhang X, et al. 2022. J Neuroinflammation. 19:111. PubMed
  41. Ye M, et al. 2022. Nat Nanotechnol. 17:880. PubMed
  42. Getts D, et al. 2014. Sci Transl Med. 15:219. PubMed
  43. Martin R, et al. 2014. J Leukoc Biol. 96:151. PubMed
  44. Pan H, et al. 2020. Mol Psychiatry. . PubMed
  45. Godbersen-Palmer C, et al. 2020. J Immunol. 204:2973. PubMed
  46. Lubkin A et al. 2019. Cell host & microbe. 25(3):463-470 . PubMed
  47. Dave K et al. 2017. eLife. 6 pii: e23382. PubMed
  48. Murphy M, et al. 2017. Eur J Immunol. 47:880. PubMed
  49. Mastandrea I, et al. 2022. STAR Protoc. 3:101106. PubMed
  50. Magod P, et al. 2021. Cell Reports. 36(5):109480. PubMed
  51. Ray A, et al. 2014. J Immunol. 192:5109. PubMed
  52. Kienzl M, et al. 2020. Oncoimmunology. 9:1776059. PubMed
  53. Werneburg S, et al. 2020. Immunity. 52(1):167-182.e7.. PubMed
  54. Anderson MC, et al. 2018. Cell Chem Biol. 25:483. PubMed
  55. Pan W et al. 2017. Immunity. 47(2):284-297 . PubMed
  56. Zhang J, et al. 2022. iScience. 25:104963. PubMed
  57. Parlet CP, et al. 2019. Cell Rep. 27:187. PubMed
  58. Green DP, et al. 2019. Neuron. 101:412. PubMed
  59. Zhang S, et al. 2019. Cell Metab. 29:443. PubMed
  60. Chakraborty S, et al. 2022. Sci Transl Med. . PubMed
  61. Li Y, et al. 2021. Brain Behav Immun. 91:267. PubMed
  62. Korangath P, et al. 2020. Bio Protoc. 10:e3822. PubMed
  63. Ajina R, et al. 2021. Cancer Immunol Res. 9:386. PubMed
  64. Ohgomori T, et al. 2020. Neuropharmacology. 176:108227. PubMed
  65. Tanaka T, et al. 2015. J Immunol. 194:2004. PubMed
  66. McLaughlin PA et al. 2019. PLoS Pathog. 15(7):e1007847 . PubMed
  67. Ye Z, et al. 2019. Mediators Inflamm. 2019:8709583. PubMed
  68. Mao FY, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:395. PubMed
  69. Morales-Mantilla DE, et al. 2022. Elife. 11:. PubMed
  70. Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
  71. Codina A, et al. 2019. Cell Syst. 8:136. PubMed
  72. Heng Y, et al. 2021. J Neuroinflammation. 18:57. PubMed
  73. Radjabova V, et al. 2015. J Immunol. 195: 3149 - 3159. PubMed
  74. Christ A, et al. 2018. Cell. 172:162. PubMed
  75. Hepler C et al. 2018. eLife. 7 pii: e39636. PubMed
  76. Kuhn JA, et al. 2021. Elife. 10:. PubMed
  77. Liu H, et al. 2022. Front Pharmacol. 13:949316. PubMed
  78. Holokai L, et al. 2020. Cancers (Basel). 12:00. PubMed
  79. Guldner IH, et al. 2020. Cell. 183(5):1234-1248.e25. PubMed
  80. Shao M, et al. 2021. Cell Stem Cell. 28(4):685-701.e7. PubMed
  81. Deczkowska A, et al. 2017. Nat Commun. 8:717. PubMed
  82. Liang J, et al. 2020. Sci Adv. 6:eabc3646. PubMed
  83. Liu X, et al. 2021. Int J Nanomedicine. 16:5675. PubMed
  84. Kolodziejczyk AA, et al. 2020. Nat Med. 26:1899. PubMed
  85. Daneshmandi S, et al. 2021. Elife. 10:. PubMed
  86. Joffin N, et al. 2021. Cell Stem Cell. 28(4):702-717.e8. PubMed
  87. Ritzel RM, et al. 2021. Glia. 69:746. PubMed
  88. Sauter K, et al. 2014. J Leukoc Biol. 96:265. PubMed
  89. Pundir P, et al. 2020. Cell Host & Microbe. 26(1):114-122. PubMed
  90. Yu W, et al. 2022. Nat Commun. 13:3544. PubMed
  91. Ryan NM, et al. 2022. Front Immunol. 13:932742. PubMed
  92. Lingegowda H, et al. 2022. Front Reprod Health. 3:726936. PubMed
  93. Melo-Silva CR, et al. 2021. PLOS Pathogens. 17(5):e1009593. PubMed
  94. Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed
  95. Zhou J, et al. 2022. Int J Oncol. 61: . PubMed
  96. Shan B, et al. 2020. Nat Metab. 1332:2. PubMed
  97. Wong NR, et al. 2021. Immunity. 54:2072. PubMed
  98. Bordas M, et al. 2020. Int J Mol Sci. 21:00. PubMed
  99. Varikuti S, et al. 2020. Br J Cancer. 122:1005. PubMed
  100. Karki R, et al. 2020. bioRxiv. . PubMed
  101. Clemente C, et al. 2018. Nat Commun. 9:910. PubMed
  102. Wang T et al. 2018. Immunity. 49(3):504-514 . PubMed
  103. Ohgomori T et al. 2019. J Comp Neurol. 528(1):14-31 . PubMed
  104. Alexander Mildner et al. 2017. Immunity. 46(5):849-862 . PubMed
  105. Li N, et al. 2022. Nat Commun. 13:7281. PubMed
  106. Liu R, et al. 2020. Eur J Vasc Endovasc Surg. 60:254. PubMed
  107. Steele NG, et al. 2021. Clin Cancer Res. 27:2023. PubMed
  108. Karki R, et al. 2021. Cell. 184:149. PubMed
  109. Huo M, et al. 2017. FASEB J. 10.1096/fj.201601030R. PubMed
  110. Mukovozov I, et al. 2015. J Immunol. 195: 3334 - 3344. PubMed
  111. Chen L, et al. 2021. Theranostics. 11:6668. PubMed
  112. Li Y, et al. 2022. Theranostics. 12:5364. PubMed
  113. Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed
  114. Molgora M, et al. 2020. Cell. 182:886. PubMed
  115. Ding L et al. 2018. Cell reports. 25(11):2972-2980 . PubMed
  116. Schaftenaar FH, et al. 2019. Sci Rep. 9:17391. PubMed
  117. Kitamoto S, et al. 2020. Cell. 182(2):447-462.e14. PubMed
  118. Kratochvill F, et al. 2015. Cancer Res. 75: 3054-3064. PubMed
  119. Pisano F, et al. 2014. PLoS One. 9:103541. PubMed
  120. Li Y, et al. 2020. Theranostics. 10:11376. PubMed
  121. Hsia HC, et al. 2017. J Leukoc Biol. 101:1053. PubMed
  122. Shin SH, et al. 2022. Clin Exp Otorhinolaryngol. . PubMed
  123. Rivera CA, et al. 2022. Immunity. 55:129. PubMed
  124. Collins C, et al. 2014. Proc Natl Acad Sci U S A. 111:9899. PubMed
  125. Tran S, et al. 2020. Immunity. 53(3):627-640.e5. PubMed
  126. Hamdan F, et al. 2021. Journal for ImmunoTherapy of Cancer. 9(8):. PubMed
  127. Tian T, et al. 2020. Cancer Immunol Res. 660:8. PubMed
  128. Ghosh S, et al. 2019. Commun Biol. 0.325. PubMed
  129. Qu J, et al. 2021. Int J Biol Sci. 17:2756. PubMed
  130. Zhang X, et al. 2020. Glia. . PubMed
  131. Kim I et al. 2015. Brain and behavior. 5(12):e00403 . PubMed
RRID
AB_1732076 (BioLegend Cat. No. 128015)
AB_1732076 (BioLegend Cat. No. 128016)

Antigen Details

Structure
14-17 kD protein (134 amino acids), member of the Ly-6 family of GPI linked protein. Ly6 family members share structure homology throughout a distinctive cystein rich protein domain that incorporates O-linked carbohydrates.
Distribution

Ly-6C is expressed primarily on bone marrow myeloid populations, monocytes/macrophages, neutrophils, endothelial cells, and some T cell subsets. Ly-6C is also a marker of memory CD8+ T cells.

Cell Type
Endothelial cells, Macrophages, Monocytes, Neutrophils, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819.
2. Cerwenka A, et al. 1998. J. Immunol. 161:97.

Gene ID
17067 View all products for this Gene ID
UniProt
View information about Ly-6C on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 12/09/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

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