- Clone
- HK1.4 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Lymphocyte antigen 6 complex, locus C
- Isotype
- Rat IgG2c, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
128031 | 125 µL | $176 | ||||
128032 | 500 µL | $396 |
Most hematopoietic cells express one or more members of Ly-6 family. The expression of Ly-6 varies with development stage and activation. Ly-6C is a 14-17 kD GPI-linked surface protein expressed on mouse monocyte/macrophage cells, endothelial cells, neutrophils, and some T cell subsets. Ly-6C is reported to be an indicator of memory CD8+ T cells.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- L3 cloned CTL cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
-
Violet Laser (405 nm)
- Application Notes
-
Clone HK1.4 does not block the binding of clone RB6-8C58.
Additional reported applications (for relevant formats of this clone) include: in vitro activation of T cells1-3 and immunohistochemistry of frozen sections4. -
Application References
(PubMed link indicates BioLegend citation) -
- Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819. (Activ)
- Herold KC, et al. 1990. Diabetes 39:815. (Activ)
- Havran WL, et al. 1988. J. Immunol. 140:1034 (Activ)
- Flanagan K, et al. 2008. J. Immunol. 180:3874. (IHC)
- Makaroff LE, et al. 2009. P. Natl. Acad. Sci. USA 106:4799. (FC)
- Zuber J, et al. 2009. Genes Dev. 23:877. (FC) PubMed
- Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
- Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
- Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
- Product Citations
-
- RRID
-
AB_2562177 (BioLegend Cat. No. 128031)
AB_2562177 (BioLegend Cat. No. 128032)
Antigen Details
- Structure
- 14-17 kD protein (134 amino acids), member of the Ly-6 family of GPI linked protein. Ly6 family members share structure homology throughout a distinctive cystein rich protein domain that incorporates O-linked carbohydrates.
- Distribution
-
Ly-6C is expressed primarily on bone marrow myeloid populations, monocytes/macrophages, neutrophils, endothelial cells, and some T cell subsets. Ly-6C is also a marker of memory CD8+ T cells.
- Cell Type
- Endothelial cells, Macrophages, Monocytes, Neutrophils, T cells
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819.
2. Cerwenka A, et al. 1998. J. Immunol. 161:97. - Gene ID
- 17067 View all products for this Gene ID
- UniProt
- View information about Ly-6C on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- What is the F/P ratio range of our BV421™ format antibody reagents?
-
It is lot-specific. On average it ranges between 2-4.
Other Formats
View All Ly-6C Reagents Request Custom ConjugationCustomers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Pacific Blue™ anti-mouse Ly-6C
C57BL/6 mouse bone marrow cells stained with HK1.4 Pacific B... -
APC anti-mouse Ly-6C
C57BL/6 bone marrow cells stained with HK1.4 APC -
Purified anti-mouse Ly-6C
C57BL/6 mouse bone marrow cells stained with purified HK1.4,... Fresh, frozen mouse spleen was stained with purified Ly6C cl... -
Biotin anti-mouse Ly-6C
BALB/c bone marrow cells stained with HK1.4 biotin, followed... -
FITC anti-mouse Ly-6C
C57BL/6 bone marrow cells stained with HK1.4 FITC (gated on ... -
Alexa Fluor® 647 anti-mouse Ly-6C
BALB/c bone marrow cells stained with HK1.4 Alexa Fluor®... -
PE anti-mouse Ly-6C
C57BL/6 bone marrow cells were stained with Ly-6C (clone HK1... -
PerCP/Cyanine5.5 anti-mouse Ly-6C
C57BL/6 bone marrow cells stained with HK1.4 PerCP/Cyanine5.... -
PE/Cyanine7 anti-mouse Ly-6C
C57BL/6 bone marrow cells stained with HK1.4 PE/Cyanine7 -
Alexa Fluor® 488 anti-mouse Ly-6C
C57BL/6 bone marrow cells stained with anti-mouse Ly-6C, HK1... -
Alexa Fluor® 700 anti-mouse Ly-6C
C57BL/6 bone marrow cells stained with HK1.4 Alexa Fluor®... -
APC/Cyanine7 anti-mouse Ly-6C
C57BL/6 mouse bone marrow cells were stained with CD8a FITC ... -
PerCP anti-mouse Ly-6C
C57BL/6 bone marrow cells were stained with Ly-6C (clone HK1... -
Brilliant Violet 570™ anti-mouse Ly-6C
C57BL/6 bone marrow cells were stained with Ly-6C (clone HK1... -
Brilliant Violet 421™ anti-mouse Ly-6C
C57BL/6 mouse bone marrow cells were stained with Ly-6C (clo... -
Brilliant Violet 510™ anti-mouse Ly-6C
C57BL/6 mouse bone marrow cells were stained with Ly-6C (clo... -
Brilliant Violet 605™ anti-mouse Ly-6C
C57BL/6 mouse bone marrow cells were stained with Ly-6C (clo... -
Brilliant Violet 711™ anti-mouse Ly-6C
C57BL/6 mouse bone marrow cells were stained with Ly-6C (clo... -
Purified anti-mouse Ly-6C (Maxpar® Ready)
Mouse splenocytes stained with 150Nd-anti-Ly-6C (HK1.4) and ... -
Brilliant Violet 785™ anti-mouse Ly-6C
C57BL/6 mouse bone marrow cells were stained with Ly-6C (clo... -
PE/Dazzle™ 594 anti-mouse Ly-6C
C57BL/6 mouse bone marrow cells were stained with Ly-6C (clo... -
APC/Fire™ 750 anti-mouse Ly-6C
C57BL/6 mouse bone marrow cells were stained with Ly-6C (clo... -
TotalSeq™-A0013 anti-mouse Ly-6C
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Brilliant Violet 650™ anti-mouse Ly-6C
C57BL/6 mouse splenocytes were stained with CD8 FITC and Ly-... -
TotalSeq™-C0013 anti-mouse Ly-6C
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TotalSeq™-B0013 anti-mouse Ly-6C
-
APC/Fire™ 810 anti-mouse Ly-6C Antibody
C57BL/6 mouse splenocytes were stained with anti-mouse CD8a ... -
Spark UV™ 387 anti-mouse Ly-6C
C57BL/6 mouse splenocytes were stained with anti-mouse CD8a ... -
PE/Fire™ 810 anti-mouse Ly-6C
C57BL/6 mouse splenocytes were stained with anti-mouse CD8a ... -
Spark Blue™ 550 anti-mouse Ly-6C (Flexi-Fluor™)
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Spark Blue™ 574 anti-mouse Ly-6C (Flexi-Fluor™)
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