Loading...

APC anti-mouse TNF-α Antibody

Pricing & Availability
Clone
MP6-XT22 (See other available formats)
Regulatory Status
RUO
Other Names
Tumor necrosis factor-α, Cachectin, Necrosin, Macrophage cytotoxic factor (MCF), Differentiation inducing factor (DIF), TNFSF-2, TNF-a, TNF-alpha
Isotype
Rat IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
MP6-XT22_APC_TNF-a_Antibody_FC_1_100713
PMA + Ionomycin-stimulated C57BL/6 mouse splenocytes (in the presence of monensin) were stained with CD3 PE, fixed, permeabilized and then stained with TNF-α (clone MP6-XT22) APC (top) or rat IgG1, κ APC isotype control (bottom).
  • MP6-XT22_APC_TNF-a_Antibody_FC_1_100713
    PMA + Ionomycin-stimulated C57BL/6 mouse splenocytes (in the presence of monensin) were stained with CD3 PE, fixed, permeabilized and then stained with TNF-α (clone MP6-XT22) APC (top) or rat IgG1, κ APC isotype control (bottom).
  • MP6-XT22_APC_TNF-a_Antibody_FC_2_100713
Compare all formats See APC spectral data
Cat # Size Price Quantity Check Availability Save
506307 25 µg $102
Check Availability


Need larger quantities of this item?
Request Bulk Quote
506308 100 µg $286
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

TNF-α is secreted by macrophages, monocytes, neutrophils, T-cells, and NK-cells. Many transformed cell lines also secrete TNF-α. Monomeric mouse TNF-α is a 156 amino acid protein (N-glycosylated) with a reported molecular weight of 17.5 kD. TNF-α forms multimeric complexes; stable trimers are most common in solution. A 26 kD membrane form of TNF-α has also been described. TNF-α binding to surface receptors elicits a wide array of biologic activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorrhagic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
E. coli-expressed, recombinant mouse TNF-α
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

ELISA or ELISPOT Detection: The biotinylated MP6-XT22 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified 6B8 antibody (Cat. Nos. 510802 & 510804) as the capture antibody.
ELISA Capture: The purified MP6-XT22 antibody is useful as the capture antibody in a sandwich ELISA when used in conjunction with the biotinylated Poly5160 antibody (Cat. No. 516003) as the detection antibody and recombinant mouse TNF-α (Cat. No. 575209) as the standard.
Flow Cytometry6,11,12:The fluorochrome-labeled MP6-XT22 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify TNF-a-producing cells within mixed cell populations.
Neutralization1,5,10,16,17:The MP6-XT22 antibody can neutralize the bioactivity of natural or recombinant TNF-α. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse TNF-α bioactivity in vivo and in vitro(Cat. No. 506310). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 506332) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).
Additional reported applications (for the relevant formats) include: Western blotting, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections7-9 in vivo detection5, immunofluorescence, and immunocytochemistry.
Note: For testing mouse TNF-α in serum, plasma or supernatant, BioLegend's ELISA Max™ Sets (Cat. No. 430901) are specially developed and recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Abrams J, et al. 1992. Immunol. Rev. 127:5. (Neut)
  2. Abrams J, et al. 1995. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.20
  3. Mo X, et al. 1995. J. Virol. 69:1288.
  4. Sarawar S, et al. 1994. J. Immunol. 153:1246.
  5. Via C, et al. 2001. J. Immunol. 167:6821. (Neut)
  6. Infante-Duarte C, et al. 2000 J. Immunol. 165:6107. (FC)
  7. Jacobs M, et al. 2000. Immunology 100:494. (IHC)
  8. Marinova-Mutachieva L, et al. 1997. Clin. Exp. Immunol. 107:507. (IHC)
  9. Williams RO, et al. 2000. J. Immunol. 165:7240. (IHC)
  10. Scanga CA, et al. 1999. Infect. Immun. 67:4531. (Neut)
  11. Akilov OE, et al. 2007. J. Leukoc. Biol. 2007;10.1189/jlb.0706439. (FC)
  12. Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC)
  13. Patole PS, et al. 2005. J. Am. Soc. Nephrol. 16:3273. PubMed
  14. Wu S, et al. 2005. Neurosci Lett. 394:158. PubMed
  15. Carlson MJ, et al. 2009. Blood 113:1365. PubMed
  16. Shivakumar P, et al. 2017. JCI Insight. 2:e88747 1. PubMed
  17. Kearney CJ, et al. 2017. Cell Death Differ. 10.1038/cdd.2017.94. PubMed
Product Citations
  1. Ozga AJ, et al. 2022. Immunity. 55:82. PubMed
  2. Yang Y, et al. 2022. FEBS J. 289:5279. PubMed
  3. Iberg CA, et al. 2022. Cell Rep. 39:110657. PubMed
  4. Jeong GU, et al. 2022. Microbiol Spectr. 10:e0109122. PubMed
  5. Chae CS, et al. 2022. Cancer Discov. 12:1904. PubMed
  6. Luo B, et al. 2022. Oxid Med Cell Longev. 2022:8965903. PubMed
  7. Yang H, et al. 2023. Cell Death Differ. 30:560. PubMed
  8. Chan L, et al. 2022. Cells. 12: . PubMed
  9. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
  10. Fiege JK, et al. 2021. Cell Host Microbe. 29:1815. PubMed
  11. Toubai T, et al. 2017. Blood Adv. 1.095138889. PubMed
  12. Yu X, et al. 2020. Nat Commun. 11:1110. PubMed
  13. Battistello E, et al. 2023. Mol Cell. 83:1216. PubMed
  14. Yang QC, et al. 2023. iScience. 26:106916. PubMed
  15. Tang M, et al. 2023. Sci Adv. 9:eade6624. PubMed
  16. Rodrigues M, et al. 2022. Biochem J. 479:1891. PubMed
  17. Koh CH, et al. 2020. Cancer Immunol Res. 8:698. PubMed
  18. Nakata J, et al. 2018. Oncotarget. 9:36029. PubMed
  19. Misumi I et al. 2019. Cell Rep. 27(5):1387-1396 . PubMed
  20. Liang Q, et al. 2022. iScience. 25:104043. PubMed
  21. Gao F, et al. 2021. Front Cell Infect Microbiol. 10:593805. PubMed
  22. Gao Y, et al. 2020. Nat Cell Biol. 1064:22. PubMed
  23. Kinder JM, et al. 2020. Cell Reports. 31(12):107784.. PubMed
  24. Oyarce C, et al. 2018. Front Immunol. 8:1794. PubMed
  25. Jones N, et al. 2021. Nat Commun. 12:1209. PubMed
  26. Koronyo-Hamaoui M, et al. 2020. Brain. 143:336. PubMed
  27. Kosack L, et al. 2017. Sci Rep. 7:11289. PubMed
  28. Misumi I et al. 2019. Cell Rep. 27(2):514-524 . PubMed
  29. Davis FM, et al. 2019. Arterioscler Thromb Vasc Biol. 39:2353. PubMed
  30. Xu X, et al. 2021. J Nanobiotechnology. 19:376. PubMed
  31. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  32. Lercher A, et al. 2019. Immunity. 51:1074. PubMed
  33. Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed
  34. Kimball A, et al. 2018. Arterioscler Thromb Vasc Biol. 38:1102. PubMed
  35. Vijay R, et al. 2015. J Exp Med. 212: 1851 - 1868. PubMed
  36. Yu C, et al. 2015. J Immunol. 195: 3890 - 3900. PubMed
  37. Forbester JL, et al. 2020. J Virol. 94:. PubMed
  38. Kimball AS et al. 2019. Immunity. 51(2):258-271 . PubMed
  39. Minamidate A, et al. 2021. Biochem Biophys Rep. 27:101065. PubMed
  40. Ulrich-Lewis JT, et al. 2022. Front Immunol. 13:861710. PubMed
  41. Darragh LB, et al. 2022. Nat Commun. 13:7015. PubMed
  42. Chua B, et al. 2011. J Immunol. 187:1692. PubMed
  43. Bullard BL, et al. 2022. NPJ Vaccines. 7:65. PubMed
  44. White C, et al. 2015. J Immunol. 194:697. PubMed
  45. Xu Y, et al. 2016. Arthritis Rheum. 67: 225-237. PubMed
  46. Buqué A, et al. 2020. Nat Commun. 3.110416667. PubMed
  47. De Ponte Conti B, et al. 2021. Elife. 10:. PubMed
  48. Tanaka Y, et al. 2020. Sci Rep. 10:17284. PubMed
  49. Ye X, et al. 2021. Cancer Biol Med. Online ahead of print. PubMed
  50. Kim MY, et al. 2017. Plant Biotechnol J. 1.729166667. PubMed
  51. Simula L et al. 2018. Cell reports. 25(11):3059-3073 . PubMed
  52. Di Mitri D, et al. 2019. Cell Rep. 28:2156. PubMed
  53. Blagih J, et al. 2020. Cell Rep. 30:481. PubMed
  54. Ge C, et al. 2020. Cell Reports. 29(13):4236-4244.e3.. PubMed
  55. Sjaastad FV, et al. 2020. Front Immunol. 11:1786. PubMed
  56. Zhang X, et al. 2021. Nat Commun. 12:4536. PubMed
  57. Raju S, et al. 2020. Cell Reports. 29(9):2556-2564.e3.. PubMed
  58. Chen Y, et al. 2022. Nat Commun. 13:4468. PubMed
  59. Kim MY, et al. 2022. Nat Commun. 13:3296. PubMed
  60. Zhang W, et al. 2021. Front Immunol. 12:768435. PubMed
  61. Asadi Shahmirzadi A, et al. 2020. Cell Metabolism. 32(3):447-456.e6. PubMed
  62. Liu Y, et al. 2018. Cancer Cell. 33:480. PubMed
  63. Dai X, et al. 2021. Molecular Cell. 81(11):2317-2331.e6. PubMed
  64. Sade–Feldman M, et al. 2018. Cell. 175:998. PubMed
  65. Ding L et al. 2018. Cell reports. 25(11):2972-2980 . PubMed
  66. Kong XF, et al. 2018. Nat Immunol. 19:973. PubMed
  67. Lee K, et al. 2011. J Biol Chem. 286:5727. PubMed
  68. Xu C, et al. 2021. Cell Reports. 35(11):109235. PubMed
  69. Li J, et al. 2021. Cell Reports. 34(11):108839. PubMed
  70. Graham JB, et al. 2017. Curr Protoc Mouse Biol. 7:221. PubMed
  71. Guo Y, et al. 2021. Nat Immunol. 22:746. PubMed
  72. Strait AA, et al. 2021. Commun Biol. 4:1005. PubMed
  73. Kim YS, et al. 2018. Front Immunol. 9:1093. PubMed
  74. Wang Y, et al. 2022. Nat Cell Biol. 24:230. PubMed
  75. Davis FM, et al. 2021. J Exp Med. 218:. PubMed
  76. Li X, et al. 2021. Front Cell Dev Biol. 9:647713. PubMed
RRID
AB_315428 (BioLegend Cat. No. 506307)
AB_315428 (BioLegend Cat. No. 506308)

Antigen Details

Structure
TNF superfamily; dimer/trimer; 17.5-150 kD (Mammalian)
Bioactivity
Paracrine/endocrine mediator of inflammatory and immune functions; selectively cytotoxic for transformed cells; endothelial cell alterations; chemoattractant
Cell Sources
Activated monocytes, neutrophils, macrophages, T cells, B cells, NK cells, LAK cells
Cell Targets
Monocytes, neutrophils, macrophages, T cells, fibroblasts, endothelial cells, osteoclasts, adipocytes, astroglia, microglia
Receptors
TNFRSF1A (TNF-R1, CD120a, TNFR-p60 Type β, p55); TNFRSF1B (TNF-R2, CD120b, TNFR-p80 Type A, p75)
Cell Type
Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Beutler B, et al. 1988. Annu. Rev. Biochem. 57:505.
3. Beutler B, et al. 1989. Annu. Rev. Immunol. 7:625.
4. Tracey K, et al. 1993. Crit. Care Med. 21:S415.

Regulation
Processed by TACE for secretion; upregulated by interferons, IL-2, GM-CSF, substance P, bradykinin, PAF, immune complexes, and cyclooxygenase; downregulated by IL-6, TGF-β, vitamin D3, prostaglandin E2, and PAF antagonists
Gene ID
21926 View all products for this Gene ID
UniProt
View information about TNF-alpha on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 10/14/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login/Register
Remember me
Forgot your password? Reset Password
Request an Account