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FITC anti-mouse TNF-α Antibody

Pricing & Availability
Clone
MP6-XT22 (See other available formats)
Regulatory Status
RUO
Other Names
Tumor necrosis factor-α, Cachectin, Necrosin, Macrophage cytotoxic factor (MCF), Differentiation inducing factor (DIF), TNFSF-2, TNF-a, TNF-alpha
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
MP6-XT22_FITC_TNF-a_Antibody_FC_1_100813
PMA + Ionomycin-stimulated C57BL/6 mouse splenocytes (in the presence of monensin) were stained with CD3 APC, fixed, permeabilized and then stained with TNF-α (clone MP6-XT22) FITC (top) or rat IgG1, κ FITC isotype control (bottom).
  • MP6-XT22_FITC_TNF-a_Antibody_FC_1_100813
    PMA + Ionomycin-stimulated C57BL/6 mouse splenocytes (in the presence of monensin) were stained with CD3 APC, fixed, permeabilized and then stained with TNF-α (clone MP6-XT22) FITC (top) or rat IgG1, κ FITC isotype control (bottom).
  • MP6-XT22_FITC_TNF-a_Antibody_FC_2_100813
Compare all formats See FITC spectral data
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506303 25 µg $67
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506304 100 µg $197
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Description

TNF-α is secreted by macrophages, monocytes, neutrophils, T-cells, and NK-cells. Many transformed cell lines also secrete TNF-α. Monomeric mouse TNF-α is a 156 amino acid protein (N-glycosylated) with a reported molecular weight of 17.5 kD. TNF-α forms multimeric complexes; stable trimers are most common in solution. A 26 kD membrane form of TNF-α has also been described. TNF-α binding to surface receptors elicits a wide array of biologic activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorrhagic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
E. coli-expressed, recombinant mouse TNF-α
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

ELISA or ELISPOT Detection: The biotinylated MP6-XT22 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified 6B8 antibody (Cat. Nos. 510802 & 510804) as the capture antibody.
ELISA Capture: The purified MP6-XT22 antibody is useful as the capture antibody in a sandwich ELISA when used in conjunction with the biotinylated Poly5160 antibody (Cat. No. 516003) as the detection antibody and recombinant mouse TNF-α (Cat. No. 575209) as the standard.
Flow Cytometry6,11,12:The fluorochrome-labeled MP6-XT22 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify TNF-a-producing cells within mixed cell populations.
Neutralization1,5,10,16,17:The MP6-XT22 antibody can neutralize the bioactivity of natural or recombinant TNF-α. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse TNF-α bioactivity in vivo and in vitro(Cat. No. 506310). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 506332) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).
Additional reported applications (for the relevant formats) include: Western blotting, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections7-9 in vivo detection5, immunofluorescence, and immunocytochemistry.
Note: For testing mouse TNF-α in serum, plasma or supernatant, BioLegend's ELISA Max™ Sets (Cat. No. 430901) are specially developed and recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Abrams J, et al. 1992. Immunol. Rev. 127:5. (Neut)
  2. Abrams J, et al. 1995. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.20
  3. Mo X, et al. 1995. J. Virol. 69:1288.
  4. Sarawar S, et al. 1994. J. Immunol. 153:1246.
  5. Via C, et al. 2001. J. Immunol. 167:6821. (Neut)
  6. Infante-Duarte C, et al. 2000 J. Immunol. 165:6107. (FC)
  7. Jacobs M, et al. 2000. Immunology 100:494. (IHC)
  8. Marinova-Mutachieva L, et al. 1997. Clin. Exp. Immunol. 107:507. (IHC)
  9. Williams RO, et al. 2000. J. Immunol. 165:7240. (IHC)
  10. Scanga CA, et al. 1999. Infect. Immun. 67:4531. (Neut)
  11. Akilov OE, et al. 2007. J. Leukoc. Biol. 2007;10.1189/jlb.0706439. (FC)
  12. Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC)
  13. Patole PS, et al. 2005. J. Am. Soc. Nephrol. 16:3273. PubMed
  14. Wu S, et al. 2005. Neurosci Lett. 394:158. PubMed
  15. Carlson MJ, et al. 2009. Blood 113:1365. PubMed
  16. Shivakumar P, et al. 2017. JCI Insight. 2:e88747 1. PubMed
  17. Kearney CJ, et al. 2017. Cell Death Differ. 10.1038/cdd.2017.94. PubMed
Product Citations
  1. Jebbawi F, et al. 2021. Parasite Immunol. 43:e12834. PubMed
  2. Lin G, et al. 2022. Int J Immunopathol Pharmacol. 36:3946320221117933. PubMed
  3. Kemna J, et al. 2023. Nat Immunol. 24:414. PubMed
  4. Jiang L, et al. 2021. Nano Today. 36:. PubMed
  5. Li Y, et al. 2020. EMBO Rep. 21:e48779. PubMed
  6. Hudson WH, et al. 2020. Immunity. 51(6):1043-1058.e4.. PubMed
  7. Chen R, et al. 2022. J Mol Cell Biol. 14:. PubMed
  8. Shi X, et al. 2023. iScience. 26:106143. PubMed
  9. Latour YL, et al. 2023. Gut Microbes. 15:2192623. PubMed
  10. Do-Thi VA, et al. 2023. Cancer Res Commun. 3:80. PubMed
  11. Tan X, et al. 2023. Adv Sci (Weinh). 10:e2206768. PubMed
  12. Park HJ, et al. 2023. NPJ Vaccines. 8:84. PubMed
  13. Murata A, et al. 2020. Front Immunol. 11:775. PubMed
  14. Wang S, et al. 2022. Front Immunol. 13:869061. PubMed
  15. Saito S, et al. 2020. Nutrients. 12:. PubMed
  16. Acharya N, et al. 2020. Immunity. 53(3):658-671.e6. PubMed
  17. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
  18. Wang C, et al. 2021. Cell Rep. 37:110021. PubMed
  19. Marangoni F, et al. 2021. Cell. . PubMed
  20. Zhang H, et al. 2020. Cancer Cell. 37(1):37-54.e9.. PubMed
  21. Bhattacharjee P, et al. 2018. Sci Rep. 13:e0199785. PubMed
  22. Wu J, et al. 2020. Immunity. 53:115. PubMed
  23. Flommersfeld S, et al. 2021. Immunity. :. PubMed
  24. Imani J, et al. 2021. JCI Insight. 6:. PubMed
  25. Grigoryan L, et al. 2022. NPJ Vaccines. 7:55. PubMed
  26. Zhao X, et al. 2015. Cytokine. 73:66. PubMed
  27. Baier FA, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:745. PubMed
  28. Hira S, et al. 2015. Cytotherapy. 17:647. PubMed
  29. Burrack K, et al. 2015. J Immunol. 194:678. PubMed
  30. Wan X, et al. 2018. Nature. 560:107. PubMed
  31. Zhu M, et al. 2015. J Immunol. 195: 4492 - 4502. PubMed
  32. Trefzer A, et al. 2021. Cell Reports. 34(6):108748. PubMed
  33. Frost JN, et al. 2021. Med (N Y). 2:164. PubMed
  34. Ma K, et al. 2022. iScience. 25:104347. PubMed
  35. Hamaidi I, et al. 2020. Cell Metabolism. 32(3):420-436.e12. PubMed
  36. Dekhtiarenko I, et al. 2016. PLoS Pathog. 12:e1006072. PubMed
  37. Herrera R, et al. 2015. Infect Immun . 83: 3771 - 3780.. PubMed
  38. Varikuti S, et al. 2020. Br J Cancer. 122:1005. PubMed
  39. Jiang L, et al. 2020. Cell. 183(5):1219-1233.e18. PubMed
  40. Sun Y, et al. 2020. J Immunol. 205:2649. PubMed
  41. SL C, et al. 2016. JCI Insight. 1: 83116. PubMed
  42. Molgora M, et al. 2020. Cell. 182:886. PubMed
  43. Chai D, et al. 2015. Antiviral Res. 119: 68-77. PubMed
  44. Jain A, et al. 2020. Nat Immunol. 0.920138889. PubMed
  45. Kerdidani D, et al. 2022. J Exp Med. 219:. PubMed
  46. Shang S, et al. 2016. PLoS Pathog. 12: 1005688. PubMed
  47. Steinmann S, et al. 2020. Sci Rep. 1.160416667. PubMed
  48. Liu Y, et al. 2021. Nat Commun. 12:6831. PubMed
  49. Kim SH, et al. 2020. Neoplasia. 1.3375. PubMed
RRID
AB_315424 (BioLegend Cat. No. 506303)
AB_315424 (BioLegend Cat. No. 506304)

Antigen Details

Structure
TNF superfamily; dimer/trimer; 17.5-150 kD (Mammalian)
Bioactivity
Paracrine/endocrine mediator of inflammatory and immune functions; selectively cytotoxic for transformed cells; endothelial cell alterations; chemoattractant
Cell Sources
Activated monocytes, neutrophils, macrophages, T cells, B cells, NK cells, LAK cells
Cell Targets
Monocytes, neutrophils, macrophages, T cells, fibroblasts, endothelial cells, osteoclasts, adipocytes, astroglia, microglia
Receptors
TNFRSF1A (TNF-R1, CD120a, TNFR-p60 Type β, p55); TNFRSF1B (TNF-R2, CD120b, TNFR-p80 Type A, p75)
Cell Type
Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Beutler B, et al. 1988. Annu. Rev. Biochem. 57:505.
3. Beutler B, et al. 1989. Annu. Rev. Immunol. 7:625.
4. Tracey K, et al. 1993. Crit. Care Med. 21:S415.

Regulation
Processed by TACE for secretion; upregulated by interferons, IL-2, GM-CSF, substance P, bradykinin, PAF, immune complexes, and cyclooxygenase; downregulated by IL-6, TGF-β, vitamin D3, prostaglandin E2, and PAF antagonists
Gene ID
21926 View all products for this Gene ID
UniProt
View information about TNF-alpha on UniProt.org
Go To Top Version: 2    Revision Date: 10/14/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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