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APC/Cyanine7 anti-mouse F4/80 Antibody

Pricing & Availability
Clone
BM8 (See other available formats)
Regulatory Status
RUO
Other Names
EMR1, Ly71
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
BM8_APCCyanine7_F4-80_Antibody_020119
Thioglycolate-elicited Balb/c mouse peritoneal macrophages were stained with CD11b FITC and F4/80 (clone BM8) APC/Cyanine7 (left) or Rat IgG2a, κ APC/Cyanine7 isotype control (right).
  • BM8_APCCyanine7_F4-80_Antibody_020119
    Thioglycolate-elicited Balb/c mouse peritoneal macrophages were stained with CD11b FITC and F4/80 (clone BM8) APC/Cyanine7 (left) or Rat IgG2a, κ APC/Cyanine7 isotype control (right).
Compare all formats See APC/Cyanine7 spectral data
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123117 25 µg $138
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123118 100 µg $325
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Description

F4/80, also known as EMR1 or Ly71, is a 160 kD glycoprotein of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80 has been widely used as a murine macrophage marker. It is expressed on a majority of tissue macrophages, including macrophages in the lung, gut, peritoneal cavity, thymus, and red pulp of the spleen, Kupffer cells, Langerhans cells, microglia, and certain dendritic cells. It is not expressed on macrophages located in the T cell areas of the spleen, lymph node, or Peyer's patch. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for the induction of CD8+ T cells-mediated peripheral tolerance.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Murine macrophages
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 1.0 µg per 106 cells in 100 µl. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections1,2 and formalin-fixed paraffin-embedded sections6,7, Western blotting, and spatial biology (IBEX)12,13.

Application References

(PubMed link indicates BioLegend citation)
  1. Schaller E, et al. 2002. Mol. Cell. Biol. 22:8035. (IHC)
  2. Stevceva L, et al. 2001. BMC Clin Pathol. 1:3. (IHC)
  3. Kobayashi M, et al.2008. J. Leukoc. Biol. 83:1354. PubMed
  4. Poeckel D, et al. 2009. J. Biol Chem. 284:21077. PubMed
  5. Glass AM, et al. 2013. J. Immunol. 190:4830. PubMed
  6. Koehm S, et al. 2007. J. Allergy Clin. Immunol. 120:570. (IHC)
  7. Rankin AL, et al. 2010. J. Immunol. 184:1526. (IHC)
  8. Sasi SP, et al. 2014. J Biol Chem. 289:14178. PubMed
  9. Thakus VS, et al. 2014. Toxicol Lett. 230:322. PubMed
  10. Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
  11. Hirakawa H, et al. 2015. PLoS One. 10:119360. PubMed
  12. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  13. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Bruand M, et al. 2021. Cell Reports. 36(3):109412. PubMed
  2. Rosina M, et al. 2022. Cell Metab. 34:533. PubMed
  3. Grandjean CL, et al. 2021. Sci Adv. 7:. PubMed
  4. Witherel CE, et al. 2021. Biomaterials. 269:120667. PubMed
  5. Zutshi S, et al. 2021. Immunology. 163:460. PubMed
  6. Fang C, et al. 2022. J Pathol. 256:310. PubMed
  7. Osokine I, et al. 2022. Cell Rep. 38:110329. PubMed
  8. Iberg CA, et al. 2022. Cell Rep. 39:110657. PubMed
  9. Guttman O, et al. 2022. J Cell Biol. 221: . PubMed
  10. Sefik E, et al. 2022. Nature. 606:585. PubMed
  11. Maccio-Maretto L, et al. 2022. Immunology. 167:64. PubMed
  12. Kraemer AN, et al. 2022. Front Immunol. 13:933191. PubMed
  13. Moiseeva V, et al. 2023. Nature. 613:169. PubMed
  14. Domingos-Pereira S, et al. 2022. Int J Mol Sci. 24: . PubMed
  15. Caslin HL, et al. 2023. Front Immunol. 13:984859. PubMed
  16. Lazure F, et al. 2023. Nat Commun. 14:535. PubMed
  17. Zhu X, et al. 2023. Front Immunol. 14:1114802. PubMed
  18. Ferriz M, et al. 2023. STAR Protoc. 4:102079. PubMed
  19. Cheng K, et al. 2023. Small. 19:e2300125. PubMed
  20. Reijers ILM, et al. 2023. J Exp Med. 220:. PubMed
  21. Ma X, et al. 2023. Nat Commun. 14:1606. PubMed
  22. Shi Y, et al. 2023. Nat Commun. 14:1884. PubMed
  23. Ludwig J, et al. 2023. NPJ Vaccines. 8:52. PubMed
  24. Taha Z, et al. 2023. Front Immunol. 14:1181014. PubMed
  25. Ferrere G, et al. 2021. JCI Insight. 6:. PubMed
  26. Resende M, et al. 2017. J Immunol. 10.4049/jimmunol.1601701. PubMed
  27. Farsakoglu Y et al. 2019. Cell reports. 26(9):2307-2315 . PubMed
  28. Pilch Z, et al. 2018. Cancer Immunol Res. 6:930. PubMed
  29. Marques RM, et al. 2021. Cell Death Differ. 28:3140. PubMed
  30. Coleby R, et al. 2021. Clin Exp Rheumatol. :39. PubMed
  31. Diao L, et al. 2022. iScience. 25:105511. PubMed
  32. Nawaz A, et al. 2022. Nat Commun. 13:7058. PubMed
  33. Andreas N, et al. 2021. Arthritis Res Ther. 23:222. PubMed
  34. Zhang S, et al. 2021. Cell. 184(8):2151-2166.e16. PubMed
  35. Vicario N, et al. 2021. Cell Death Disease. 12(7):625. PubMed
  36. Culina S, et al. 2014. PLoS One. 9:88365. PubMed
  37. Kozijn AE, et al. 2019. Osteoarthritis Cartilage. 27:118. PubMed
  38. Zhao J, et al. 2019. Nat Commun. 10:899. PubMed
  39. Ushio A, et al. 2017. Int J Mol Sci. 10.3390/ijms18061209. PubMed
  40. Wang X, et al. 2020. Signal Transduct Target Ther. 5:35. PubMed
  41. Bode K, et al. 2020. Cell Reports. 29(13):4435-4446.e9.. PubMed
  42. Rybalko V, et al. 2015. PLoS One. 10: 0145550. PubMed
  43. Ohtsuki T, et al. 2016. J Virol. 90: 300 - 307. PubMed
  44. Nguyen SL, et al. 2021. Sci Rep. 11:4217. PubMed
  45. Li S, et al. 2021. Ann Transl Med. 9:1644. PubMed
  46. Dias AMM, et al. 2021. J Gastroenterol. 56:442. PubMed
  47. Wang Y, et al. 2022. Front Immunol. 13:767630. PubMed
  48. Han YH, et al. 2021. Science. 373:. PubMed
  49. Forni MF, et al. 2021. Front Oncol. 11:667715. PubMed
  50. Chen C, et al. 2017. Cell Rep. 10.1016/j.celrep.2017.02.040. PubMed
  51. Herbst S, et al. 2013. Dis Model Mech. 6:643. PubMed
  52. Zhang D, et al. 2020. Signal Transduct Target Ther. 5:24. PubMed
  53. Brykczynska U, et al. 2020. Cell Reports. 30(5):1627-1643.e7.. PubMed
  54. Ohta S, et al. 2013. J Immunol. 190:5927. PubMed
  55. Mason K, et al. 2013. J Immunol. 191:2457. PubMed
  56. Levine LS, et al. 2021. Immunity. 54(4):829-844.e5. PubMed
  57. Li J, et al. 2020. Cancer Discov. . PubMed
  58. Haase C, et al. 2022. Nat Methods. 19:1622. PubMed
  59. Peruzzotti-Jametti L, et al. 2021. PLoS Biol. 19:e3001166. PubMed
  60. Li J, et al. 2020. Cancer Immunol Res. 0.529166667. PubMed
  61. Taguchi K, et al. 2015. J Urol. . PubMed
  62. Benechet AP, et al. 2019. Nature. 574:200. PubMed
  63. Domingos-Pereira S, et al. 2019. Cancer Immunol Res. 7:621. PubMed
  64. Adams NM et al. 2018. Immunity. 48(6):1172-1182 . PubMed
  65. Li J, et al. 2018. Immunity. 49:178. PubMed
  66. Diaz–Salazar C, et al. 2020. Cell Rep. 32:108186. PubMed
  67. Taguchi K, et al. 2014. J Am Soc Nephrol. 25:1680. PubMed
  68. Wiedemann GM, et al. 2020. Cell Rep. 33:108498. PubMed
  69. Gao S, et al. 2022. Cell Res. 32:38. PubMed
  70. Pitsch J, et al. 2021. Ann Neurol. 89:666. PubMed
  71. Yang M, et al. 2021. Cancer Immunol Immunother. 70:2467. PubMed
  72. Bayik D, et al. 2020. Cancer Discov. 1.256944444. PubMed
  73. Xue N, et al. 2017. Sci Rep. 7:39011. PubMed
  74. Watanabe E, et al. 2020. Angiogenesis. 23(4):667-684. PubMed
  75. Haloul M, et al. 2019. Sci Rep. 9:14050. PubMed
  76. Guo M, et al. 2018. J Immunol. 200:1039. PubMed
  77. Habib S, et al. 2018. Infect Immun. 86:e00019. PubMed
  78. Pilch Z, et al. 2019. PLoS One. 14:e0210796. PubMed
  79. Sasaki T, et al. 2020. Cell Reports. 28(1):202-217.e7.. PubMed
  80. Liang J, et al. 2020. Sci Adv. 6:eabc3646. PubMed
  81. Sharma A, et al. 2020. Cell. 183(2):377-394.e21. PubMed
  82. De Simone G, et al. 2021. Immunity. :. PubMed
  83. Bugler-Lamb AR, et al. 2021. Mol Metab. 49:101197. PubMed
  84. Grioni M, et al. 2021. Blood Adv. 5:2817. PubMed
  85. Bourque J, et al. 2021. Heliyon. 7:e08311. PubMed
  86. Shen M, et al. 2022. Nat Cancer. 3:60. PubMed
  87. Blriot C, et al. 2021. Immunity. :. PubMed
  88. Ni J, et al. 2020. Immunity. 52(6):1075-1087.e8. PubMed
  89. Zordan P, et al. 2013. Eur J Pharmacol. 715:296. PubMed
  90. Carestia A, et al. 2019. Cell Rep. 28:896. PubMed
  91. Lebel MÈ, et al. 2020. Nat Commun. 3.051388889. PubMed
  92. Georgoudaki A, et al. 2016. Cell Rep. 15: 2000-2011. PubMed
  93. Kolodkin-Gal D, et al. 2021. Gut. Online ahead of print. PubMed
  94. Taddio MF, et al. 2021. Mol Imaging Biol. 23:196. PubMed
  95. Nuro-Gyina PK, et al. 2021. J Immunol. 207:868. PubMed
  96. Takahashi F, et al. 2022. iScience. 25:104278. PubMed
  97. Bagadia P, et al. 2019. Nat Immunol. 20:1174. PubMed
  98. Moro T, et al. 2016. PLoS One. 11: 0146592. PubMed
  99. Dichtl S, et al. 2022. Life Sci Alliance. 5:. PubMed
  100. Zhu K, et al. 2022. EMBO Rep. 23:e54499. PubMed
  101. Xu JL, et al. 2022. Front Pharmacol. 13:911663. PubMed
  102. de Assis LVM, et al. 2022. Commun Biol. 5:461. PubMed
  103. Zhang W, et al. 2021. Front Immunol. 12:768435. PubMed
  104. Ni Y, et al. 2021. Theranostics. 11:9431. PubMed
  105. Casselli T, et al. 2021. PLoS Pathog. 17:e1009256. PubMed
  106. Bergquist M, et al. 2014. J Infect. 69:113. PubMed
  107. Natale CA, et al. 2018. Elife. 7. PubMed
  108. Peruzzotti-Jametti L, et al. 2018. Cell Stem Cell. 22:355. PubMed
  109. Leiria LO et al. 2019. Cell Metab. 30(4):768-783 . PubMed
  110. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  111. Klose R, et al. 2016. Nat Commun. 7:12528. PubMed
  112. Zhang D, et al. 2020. Signal Transduct Target Ther. 5:24. PubMed
  113. Fedele C, et al. 2021. J Exp Med. 218: . PubMed
  114. Zhang Z, et al. 2020. Front Immunol. 11:583276. PubMed
  115. Smith KJ, et al. 2022. PLoS Biol. 20:e3001554. PubMed
  116. Deliyannis G, et al. 2021. JCI Insight. 6:. PubMed
  117. Wang M, et al. 2021. iScience. 24(7):102766. PubMed
  118. Kwok I, et al. 2020. Immunity. 53(2):303-318.e5. PubMed
  119. DeFalco T, et al. 2016. Cell Rep. 12: 1107-1119. PubMed
  120. Bertuzzi M, et al. 2014. PLoS Pathog. 10:1004413. PubMed
  121. Celià-Terrassa T, et al. 2017. Nat Cell Biol. 19:711. PubMed
  122. Geary CD et al. 2018. Cell reports. 24(8):1949-1957 . PubMed
  123. Mellal K, et al. 2019. Sci Rep. 9:12903. PubMed
  124. Herbst S, et al. 2015. EMBO Mol Med. 30:240. PubMed
  125. Cardoso M, et al. 2015. Infect Immun . 83: 3534-3544. PubMed
  126. Chen Z, et al. 2016. Innate Immunity. 22: 419 - 432. PubMed
  127. Kahn JH, et al. 2020. Endocrinology. 161(10):. PubMed
  128. Patterson DG, et al. 2021. J Immunol. 207:1798. PubMed
  129. Andreata F, et al. 2021. STAR Protoc. 2:100831. PubMed
  130. Ren X, et al. 2021. Cell Death Dis. 12:484. PubMed
  131. Sameshima A, et al. 2015. J Endocrinol. 227: 25-36. PubMed
  132. Morrissey MA, et al. 2018. Elife. 7:e36688. PubMed
  133. Yáñez A et al. 2017. Immunity. 47(5):890-902 . PubMed
RRID
AB_893477 (BioLegend Cat. No. 123117)
AB_893477 (BioLegend Cat. No. 123118)

Antigen Details

Structure
EGF-TM7 family member, 160 kD glycoprotein
Distribution

Majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen, Kupffer cells, Langerhans cells, bone marrow stromal cells, and a subset of dendritic cells

Function
Induction of immunological tolerance
Cell Type
Dendritic cells, Langerhans cells, Macrophages, Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Antigen References

1. Austy JM and Gordon S. 1981. Eur. J. Immunol. 11:805.
2. Hume DA, et al. 1983. J. Exp. Med. 158:1522.
3. Ruedl C, et al. 1996. Eur. J. Immunol. 26:1801.
4. McKnight AJ, et al. 1996. J. Biol. Chem. 271:486.
5. Lin HH, et al. 2005. J. Exp. Med. 201:1615.

Gene ID
13733 View all products for this Gene ID
UniProt
View information about F4/80 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All F4/80 Reagents Request Custom Conjugation
Description Clone Applications
Brilliant Violet 605™ anti-mouse F4/80 BM8 FC
Purified anti-mouse F4/80 BM8 FC,IHC,WB
Biotin anti-mouse F4/80 BM8 FC,IHC
FITC anti-mouse F4/80 BM8 FC
PE anti-mouse F4/80 BM8 FC
PE/Cyanine5 anti-mouse F4/80 BM8 FC
PE/Cyanine7 anti-mouse F4/80 BM8 FC
APC anti-mouse F4/80 BM8 FC
APC/Cyanine7 anti-mouse F4/80 BM8 FC
Alexa Fluor® 488 anti-mouse F4/80 BM8 FC,IHC-F,3D IHC
Alexa Fluor® 647 anti-mouse F4/80 BM8 FC,IHC-F,3D IHC
Pacific Blue™ anti-mouse F4/80 BM8 FC
PerCP anti-mouse F4/80 BM8 FC
PerCP/Cyanine5.5 anti-mouse F4/80 BM8 FC
Alexa Fluor® 700 anti-mouse F4/80 BM8 FC
Brilliant Violet 421™ anti-mouse F4/80 BM8 FC,IHC-F,SB
Brilliant Violet 510™ anti-mouse F4/80 BM8 FC
Alexa Fluor® 594 anti-mouse F4/80 BM8 IHC-F
Brilliant Violet 785™ anti-mouse F4/80 BM8 FC
Purified anti-mouse F4/80 (Maxpar® Ready) BM8 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse F4/80 BM8 FC
Brilliant Violet 650™ anti-mouse F4/80 BM8 FC
Brilliant Violet 711™ anti-mouse F4/80 BM8 FC
APC/Fire™ 750 anti-mouse F4/80 BM8 FC
TotalSeq™-A0114 anti-mouse F4/80 BM8 PG
TotalSeq™-B0114 anti-mouse F4/80 BM8 PG
TotalSeq™-C0114 anti-mouse F4/80 BM8 PG
Spark YG™ 570 anti-mouse F4/80 BM8 IHC-F
KIRAVIA Blue 520™ anti-mouse F4/80 BM8 FC
Ultra-LEAF™ Purified anti-mouse F4/80 BM8 FC,IHC,WB
APC/Fire™ 810 anti-mouse F4/80 BM8 FC
Spark NIR™ 685 anti-mouse F4/80 BM8 FC
Spark Blue™ 550 anti-mouse F4/80 BM8 FC
Brilliant Violet 570™ anti-mouse F4/80 BM8 FC
Brilliant Violet 750™ anti-mouse F4/80 BM8 FC
PE/Fire™ 810 anti-mouse F4/80 BM8 FC
Spark Red™ 718 anti-mouse F4/80 (Flexi-Fluor™) BM8 FC
Spark Blue™ 574 anti-mouse F4/80 (Flexi-Fluor™) BM8 FC
Go To Top Version: 3    Revision Date: 02/01/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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