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Brilliant Violet 421™ anti-human CD25 Antibody

Pricing & Availability
Clone
BC96 (See other available formats)
Regulatory Status
RUO
Workshop
V T-072
Other Names
Low affinity IL-2R, IL-2R α chain, Tac, p55
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
BC96_BV421_1_032911
Human peripheral blood lymphocytes were stained with CD4 FITC and CD25 (clone BC96) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom).
  • BC96_BV421_1_032911
    Human peripheral blood lymphocytes were stained with CD4 FITC and CD25 (clone BC96) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom).
  • BC96_BV421_2_032911
Compare all formats See Brilliant Violet 421™ spectral data
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302629 25 tests $185
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302630 100 tests $381
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Description

CD25 is a 55 kD type I transmembrane glycoprotein also known as the low affinity IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes/macrophages. CD25 is also expressed on a subset of non-stimulated CD4+ T cells termed T regulatory cells. CD25 associates with the IL-2 receptor β (CD122) and common γ chains (CD132) to form the high affinity IL-2R complex.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Baboon, Chimpanzee, Cynomolgus, Pigtailed Macaque, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications include: immunocytochemistry3.

Additional Product Notes

View more applications data for this product in our Scientific Poster Library.

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
  3. Ernst CW, et al. 2007. Clin. Exp. Immunol. 148:271. (ICC) PubMed
Product Citations
  1. Ellis GI, et al. 2022. Cell Rep Med. 3:100614. PubMed
  2. Ringelstein-Harlev S, et al. 2023. Eur J Haematol. 110:371. PubMed
  3. Hsiao CC, et al. 2023. iScience. 26:105785. PubMed
  4. Zhang C, et al. 2023. Front Immunol. 14:1182409. PubMed
  5. Marco Barros R, et al. 2016. Cell. 167: 203-218. PubMed
  6. Shoda H, et al. 2017. Arthritis Research & Therapy . 10.1186/s13075-017-1308-y. PubMed
  7. Nagafuchi Y, et al. 2016. Sci Rep. 6:29338. PubMed
  8. van der Heiden M, et al. 2020. Scand J Immunol. e13005:. PubMed
  9. Nesspor TC, et al. 2020. Sci Rep. 10:7557. PubMed
  10. Huang C, et al. 2020. Theranostics. 10:10498. PubMed
  11. Pan YG, et al. 2021. Immunity. 54(6):1245-1256.e5. PubMed
  12. Torres-Ruiz J, et al. 2021. Front Immunol. 12:689966. PubMed
  13. Herter JM, et al. 2022. Strahlenther Onkol. Online ahead of print. PubMed
  14. Lam AJ, et al. 2021. Cell Reports. 36(5):109494. PubMed
  15. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  16. Pagel J, et al. 2020. Front Immunol. 11:565257. PubMed
  17. Jürchott K, et al. 2016. PLoS One. 11: 0150812. PubMed
  18. Yang L, et al. 2020. Genes Dis. 7:128. PubMed
  19. Ellis GI, et al. 2022. STAR Protoc. 3:101784. PubMed
  20. Idorn M, et al. 2018. Oncoimmunology. 7:e1412029. PubMed
  21. Nowak A, et al. 2018. Front Immunol. 0.513194444. PubMed
  22. Gao X, et al. 2022. iScience. 25:104911. PubMed
  23. Nguyen J, et al. 2021. Mol Syst Biol. 17:e10560. PubMed
  24. Paul S, et al. 2021. Sci Transl Med. 13:. PubMed
  25. Nakachi S, et al. 2017. Arthritis Research & Therapy . 10.1186/s13075-017-1309-x. PubMed
  26. Janssen JJE, et al. 2022. Am J Physiol Endocrinol Metab. 322:E141. PubMed
  27. Hernndez DC, et al. 2021. Immunity. :. PubMed
  28. Pandya J, et al. 2016. J Leukoc Biol. 100: 823 - 833. PubMed
  29. Manuszak C, et al. 2020. J Biol Methods. 7:e131. PubMed
  30. Li H, et al. 2020. Front Oncol. 1.477083333. PubMed
  31. Nowatzky J, et al. 2020. J Vis Exp. . PubMed
  32. Fu J, et al. 2021. J Clin Invest. 131:. PubMed
  33. Lin-Shiao E, et al. 2022. Nucleic Acids Res. 50:1256. PubMed
RRID
AB_10896914 (BioLegend Cat. No. 302629)
AB_10896914 (BioLegend Cat. No. 302630)

Antigen Details

Structure
Type I transmembrane glycoprotein, 55 kD
Distribution

Activated T cells and B cells, monocytes/macrophages, Treg

Function
Associates with IL-2 receptor β (CD122) and γ chains (CD132) to form high affinity IL-2R complex
Ligand/Receptor
IL-2
Cell Type
B cells, Macrophages, Monocytes, T cells, Tregs
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Taniguchi T, et al. 1993. Cell 73:5.
2. Waldmann T. 1991. J. Biol. Chem. 266:2681.

Gene ID
3559 View all products for this Gene ID
UniProt
View information about CD25 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 3    Revision Date: 12/15/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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