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FITC anti-human CD45RA Antibody

Pricing & Availability
Clone
HI100 (See other available formats)
Regulatory Status
RUO
Workshop
IV N906
Other Names
GP180, L-CA, LCA, LY5, T200, PTPRC
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
HI100
Human peripheral blood lymphocytes stained with HI100 FITC
  • HI100
    Human peripheral blood lymphocytes stained with HI100 FITC
Compare all formats See FITC spectral data
Cat # Size Price Quantity Check Availability Save
304105 25 tests $28
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304106 100 tests $66
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304148 100 µg $129
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Description

CD45RA is a 205-220 kD single chain type I glycoprotein. It is an exon 4 splice variant of the tyrosine phosphatase CD45. The CD45RA isoform is expressed on resting/naïve T cells, medullary thymocytes, B cells and monocytes. CD45RA enhances both T cell receptor and B cell receptor signaling. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4. CD45 has also been reported to bind galectin-1. CD45 isoform expression can change in response to cytokines.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
µg sizes: 0.5 mg/mL
test sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for relevant formats of this clone) include: inhibition of CD45 functions2, immunohistochemical staining of frozen tissue sections3 and formalin-fixed paraffin-embedded tissue sections4, and immunocytochemistry15,16.

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Yamada T, et al. 2002. J. Biol. Chem. 277:28830. (WB, Block)
  3. Weninger W, et al. 2003 J. Immunol. 170:4638. (IHC-F)
  4. Imanguli MM, et al. 2009. Blood. 113:3620 (IHC-P)
  5. Roque S, et al. 2007. J. Immunol. 178:8028. (FC) PubMed
  6. Smeltz RB. 2007. J. Immunol. 178:4786. (FC) PubMed
  7. Palendira U, et al. 2008. Blood (FC) PubMed
  8. Kuttruff S, et al. 2009. Blood 113:358. (FC) PubMed
  9. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
  10. Alanio C, et al. 2010. Blood 115:3718. (FC) PubMed
  11. Iannello A, et al. 2010. J. Immunol. 184:114. (FC) PubMed
  12. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  13. Guereau-de-Arellan M, et al. 2011. Brain. 134:3578. PubMed
  14. Canque B, et al. 2000. Blood 96:3748. (ICC)
  15. Imanguli MM, et al. 2009. Blood 13:3620. (ICC)
  16. Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
  17. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations
  1. Iwahori K, et al. 2019. Sci Rep. 2.205555556. PubMed
  2. Cirovic B, et al. 2020. Cell Host & Microbe. 28(2):322-334. PubMed
  3. Yuan H, et al. 2022. J Clin Exp Hematop. 62:52. PubMed
  4. Ogura H, et al. 2022. Nat Commun. 13:7063. PubMed
  5. Li M, et al. 2023. Front Immunol. 14:1087923. PubMed
  6. Ryan FJ, et al. 2023. Cell Rep Med. 4:100971. PubMed
  7. Li D, et al. 2023. Int J Oncol. 62:. PubMed
  8. Ge Y, et al. 2016. Diabetes. 65: 794 - 802. PubMed
  9. Bachmann M, et al. 2012. J Biol Chem. 287:41684. PubMed
  10. Sun J, et al. 2018. Scand J Immunol. 88:e12674. PubMed
  11. Kim CJ, et al. 2018. Immunity. 49:1034. PubMed
  12. Webb LMC, et al. 2021. Aging Cell. 20:e13295. PubMed
  13. Hess NJ, et al. 2020. J Immunol. 205:272. PubMed
  14. Gao Y, et al. 2021. Oncogenesis. 10:62. PubMed
  15. Acharya N, et al. 2020. Immunity. 53(3):658-671.e6. PubMed
  16. Weithauser A, et al. 2013. J Am Coll Cardiol. 62:1737. PubMed
  17. Mori Y, et al. 2015. Proc Natl Acad Sci U S A. 112: 9638-9643. PubMed
  18. Izumi K, et al. 2021. Immunohorizons. 5:687. PubMed
  19. Kan S, et al. 2020. Int J Oncol. 57:1047. PubMed
  20. Ramaswamy A, et al. 2021. Immunity. 54(5):1083-1095.e7. PubMed
  21. Sandborn WJ, et al. 2021. Gastroenterology. 161:1853. PubMed
  22. Toews K, et al. 2020. Mol Carcinog. 724:59. PubMed
  23. Obradovic A, et al. 2021. Cell. 184(11):2988-3005.e16. PubMed
  24. Kuebler P, et al. 2015. Proc Natl Acad Sci U S A. 112: 8379 - 8384. PubMed
  25. Osorio FG et al. 2018. Cell reports. 25(9):2308-2316 . PubMed
  26. Bharath LP, et al. 2020. Cell Metabolism. 32(1):44-55.e6.. PubMed
  27. Van Egeren D, et al. 2021. Cell Stem Cell. 28(3):514-523.e9. PubMed
  28. Arcangeli S, et al. 2022. J Clin Invest. 132: . PubMed
  29. Li Q, et al. 2012. J Leukoc Biol. 91:311. PubMed
  30. Zhu S, et al. 2022. J Oncol. 2022:8724933. PubMed
  31. Su X, et al. 2022. J Transl Med. 20:378. PubMed
  32. Theobald SJ, et al. 2022. EMBO Mol Med. 14:e15888. PubMed
  33. Szklarski M, et al. 2021. Front Immunol. 12:644548. PubMed
  34. Dos Santos JC, et al. 2019. Cell Rep. 28:2659. PubMed
  35. Cesana M, et al. 2018. Cell Stem Cell. 22:575. PubMed
  36. Yuan J et al. 2018. Molecular cell. 72(2):380-394 . PubMed
  37. Kubin ME, et al. 2017. Acta Derm Venereol. 97:449. PubMed
  38. Lima J, et al. 2016. Reprod Sci. 10.1177/1933719116653680. PubMed
  39. Vadrevu KM, et al. 2022. Sci Rep. 12:12038. PubMed
  40. Rabold K, et al. 2022. Nat Commun. 13:6149. PubMed
  41. Lancioni C, et al. 2011. Infect Immun. 79:663. PubMed
  42. Smith-Díaz CC, et al. 2021. Front Oncol. 11:709543. PubMed
  43. Isobe T, et al. 2022. Nat Commun. 13:4501. PubMed
  44. Burke MT, et al. 2017. Transplant Direct. 3:e171. PubMed
  45. Zhao B, et al. 2013. PLoS One. 8:77708. PubMed
  46. Heckler M, et al. 2021. Cancer Discov. Online ahead of prin. PubMed
  47. Tao L, et al. 2020. Cancers (Basel). 12:00. PubMed
  48. Mackroth M, et al. 2016. PLoS Pathog. 12:e1005909. PubMed
  49. Steindor M, et al. 2015. PLoS One. 10:119737. PubMed
  50. Serr I, et al. 2016. Nat Commun. 7:10991. PubMed
  51. Li M, et al. 2020. Nat Commun. 4051:11. PubMed
RRID
AB_314410 (BioLegend Cat. No. 304105)
AB_314410 (BioLegend Cat. No. 304106)
AB_314410 (BioLegend Cat. No. 304148)

Antigen Details

Structure
Tyrosine phosphatases, type I transmembrane (exon 4 splicing of CD45 gene), 205-220 kD
Distribution

B cells, naïve T cells, monocytes

Function
Enhances TCR and BCR signaling
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Cell Type
B cells, Monocytes, T cells, Tregs
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Thomas M. 1989. Annu. Rev. Immunol. 7:339.
2. Trowbridge I, et al. 1994. Annu. Rev. Immunol.12:85.

Gene ID
5788 View all products for this Gene ID
UniProt
View information about CD45RA on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD45RA Reagents Request Custom Conjugation
Description Clone Applications
APC anti-human CD45RA HI100 FC
Biotin anti-human CD45RA HI100 FC
FITC anti-human CD45RA HI100 FC
PE anti-human CD45RA HI100 FC,SB
PE/Cyanine5 anti-human CD45RA HI100 FC
Purified anti-human CD45RA HI100 FC,CyTOF®,ICC,IHC-P,IHC-F,SB
Alexa Fluor® 488 anti-human CD45RA HI100 FC
Alexa Fluor® 647 anti-human CD45RA HI100 FC,IHC-P
Pacific Blue™ anti-human CD45RA HI100 FC
Alexa Fluor® 700 anti-human CD45RA HI100 FC
PerCP/Cyanine5.5 anti-human CD45RA HI100 FC,SB
PE/Cyanine7 anti-human CD45RA HI100 FC
APC/Cyanine7 anti-human CD45RA HI100 FC
Brilliant Violet 421™ anti-human CD45RA HI100 FC,IHC-P
Brilliant Violet 570™ anti-human CD45RA HI100 FC
Brilliant Violet 605™ anti-human CD45RA HI100 FC
Brilliant Violet 650™ anti-human CD45RA HI100 FC
Brilliant Violet 711™ anti-human CD45RA HI100 FC
Brilliant Violet 785™ anti-human CD45RA HI100 FC
Brilliant Violet 510™ anti-human CD45RA HI100 FC
Purified anti-human CD45RA (Maxpar® Ready) HI100 FC,CyTOF®
PE/Dazzle™ 594 anti-human CD45RA HI100 FC
APC/Fire™ 750 anti-human CD45RA HI100 FC
PerCP anti-human CD45RA HI100 FC
FITC anti-human CD45RA HI100 FC
TotalSeq™-A0063 anti-human CD45RA HI100 PG
Alexa Fluor® 594 anti-human CD45RA HI100 IHC-P
TotalSeq™-B0063 anti-human CD45RA HI100 PG
TotalSeq™-C0063 anti-human CD45RA HI100 PG
Brilliant Violet 750™ anti-human CD45RA HI100 FC
Spark NIR™ 685 anti-human CD45RA HI100 FC
APC anti-human CD45RA HI100 FC
PE/Fire™ 640 anti-human CD45RA HI100 FC
PE/Fire™ 700 anti-human CD45RA Antibody HI100 FC
Spark YG™ 581 anti-human CD45RA HI100 FC
TotalSeq™-D0063 anti-human CD45RA HI100 PG
Spark Violet™ 423 anti-human CD45RA HI100 FC,IHC-P
GMP FITC anti-human CD45RA HI100 FC
PE/Cyanine7 anti-human CD45RA HI100 FC
PE/Dazzle™ 594 anti-human CD45RA HI100 FC
APC/Fire™ 750 anti-human CD45RA HI100 FC
Spark UV™ 387 anti-human CD45RA HI100 FC
GMP APC anti-human CD45RA HI100 FC
TotalSeq™-Bn0063 anti-human CD45RA HI100 SB
Spark Blue™ 550 anti-human CD45RA HI100 FC
GMP PE/Dazzle™ 594 anti-human CD45RA HI100 FC
GMP APC/Fire™ 750 anti-human CD45RA HI100 FC
Spark PLUS UV395™ anti-human CD45RA HI100 FC
Spark Red™ 718 anti-human CD45RA HI100 FC
APC/Fire™ 810 anti-human CD45RA HI100 FC
PE/Fire™ 810 anti-human CD45RA HI100 FC
Spark Blue™ 574 anti-human CD45RA (Flexi-Fluor™) HI100 FC
Go To Top Version: 3    Revision Date: 08/26/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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