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Brilliant Violet 421™ anti-mouse CD304 (Neuropilin-1) Antibody

Pricing & Availability
Clone
3E12 (See other available formats)
Regulatory Status
RUO
Other Names
NRP-1, VEGFR165 receptor
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
3E12_BV421_CD304_Antibody_FC_1_080213.jpg
C57BL/6 mouse splenocytes were stained with CD4 APC and CD304 (clone 3E12) Brilliant Violet 421™ (top) or rat IgG2a, κ Brilliant Violet 421™ isotype control (bottom).
  • 3E12_BV421_CD304_Antibody_FC_1_080213.jpg
    C57BL/6 mouse splenocytes were stained with CD4 APC and CD304 (clone 3E12) Brilliant Violet 421™ (top) or rat IgG2a, κ Brilliant Violet 421™ isotype control (bottom).
  • 3E12_BV421_CD304_Antibody_FC_2_080213.jpg
Compare all formats See Brilliant Violet 421™ spectral data
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145209 125 µL $198
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Description

CD304, also known as neuropilin-1, is a 140 kD type I transmembrane protein. Its extracellular region contains two CUB, two FV/FVIII, and one MAM domain. It is expressed by natural regulatory T cells (nTreg), a subset of invariant natural killer T cells (iNKT), endothelial cells, and neurons. Neuropilin-1 stabilizes the interaction between Tregs and dendritic cells, contributes to the recruitment of tumor-infiltrating Tregs in response to tumor-derived VEGF, and influences the process of angiogenesis and axon guidance. The ligands of CD304 are VEGF165 and semaphorin-3A.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Extracellular region of mouse CD304
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Blankenhaus B, et al. 2014. PLoS Pathog. 10:1003913. PubMed
  2. Verhagen J and Wraith DC. 2014. J. Immunol. Methods. S0022. (FC) PubMed
  3. Verhagen J, et al. 2014. PLoS One. 9e:108023. (FC) PubMed
Product Citations
  1. Brown HM, et al. 2018. Sci Rep. 1.801388889. PubMed
  2. Martens PJ, et al. 2022. Front Endocrinol (Lausanne). 13:1023264. PubMed
  3. Huang W, et al. 2014. J Immunol. 193:2267. PubMed
  4. Sasaki K, et al. 2019. Nat Commun. 10:3878. PubMed
  5. Dikiy S, et al. 2021. Immunity. 54(5):931-946.e11. PubMed
  6. Grzelak A, et al. 2018. Int J Mol Sci. 19:. PubMed
  7. Yue X, et al. 2019. Nat Commun. 10:2011. PubMed
  8. Overacre-Delgoffe AE, et al. 2017. Cell. 169:1130. PubMed
  9. Sharma M, et al. 2020. Circ Res. 127:335. PubMed
  10. Kyburz A, et al. 2019. J Allergy Clin Immunol. 143:1496. PubMed
  11. Leclerc M, et al. 2019. Nat Commun. 10:3345. PubMed
  12. Matsuda T, et al. 2022. iScience. 25:105324. PubMed
  13. Sun F, et al. 2022. Cell Death Dis. 13:181. PubMed
  14. Scheyltjens I, et al. 2022. Nat Protoc. 17:2354. PubMed
  15. Bastow CR, et al. 2022. Front Immunol. 13:873586. PubMed
RRID
AB_2562358 (BioLegend Cat. No. 145209)

Antigen Details

Structure
Type I transmembrane protein, 140 kD, 2 complement binding domains (CUB), 2 coagulation factor V/VIII homology domains (FV/FVIII), one meprin, A5, receptor tyrosine phosphatase domain (MAM)
Distribution

Natural regulatory T cells (nTreg), subset of invariant natural killer T cells (iNKT), endothelial cells, neurons

Function
Angiogenesis, axon guidance, modulation of the immune response
Ligand/Receptor
VEGF165, semaphorin-3A
Cell Type
Dendritic cells, Endothelial cells, Neurons, NKT cells, Tregs
Biology Area
Angiogenesis, Cancer Biomarkers, Cardiovascular Biology, Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Synaptic Biology
Molecular Family
Adaptor Proteins, Adhesion Molecules, CD Molecules
Antigen References

1. Yadav M, et al. 2012. J. Exp. Med. 209:1713.
2. Weiss JM, et al. 2012. J. Exp. Med. 209:1723.
3. Hansen W, et al. 2012. J. Exp. Med. 209:2001.
4. Milpied P, et al. 2011. Blood 118:2993.

Gene ID
18186 View all products for this Gene ID
UniProt
View information about CD304 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 1    Revision Date: 08/05/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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