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Purified anti-mouse CD304 (Neuropilin-1) Antibody

Pricing & Availability
Clone
3E12 (See other available formats)
Regulatory Status
RUO
Other Names
NRP-1, VEGFR165 receptor
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
3E12_Purified_CD304_Antibody_FC_1_021113
C57BL/6 mouse splenocytes were stained with purified CD304 (clone 3E12, top) or purified rat IgG2a, κ isotype control (bottom), followed by anti-rat IgG PE. Splenocytes were then stained with mouse CD4 APC.
  • 3E12_Purified_CD304_Antibody_FC_1_021113
    C57BL/6 mouse splenocytes were stained with purified CD304 (clone 3E12, top) or purified rat IgG2a, κ isotype control (bottom), followed by anti-rat IgG PE. Splenocytes were then stained with mouse CD4 APC.
  • 3E12_Purified_CD304_Antibody_FC_2_021113
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145201 25 µg $124
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Description

CD304, also known as neuropilin-1, is a 140 kD type I transmembrane protein. Its extracellular region contains two CUB, two FV/FVIII, and one MAM domain. It is expressed by natural regulatory T cells (nTreg), a subset of invariant natural killer T cells (iNKT), endothelial cells, and neurons. Neuropilin-1 stabilizes the interaction between Tregs and dendritic cells, contributes to the recruitment of tumor-infiltrating Tregs in response to tumor-derived VEGF, and influences the process of angiogenesis and axon guidance. The ligands of CD304 are VEGF165 and semaphorin-3A.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Extracellular region of mouse CD304
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Blankenhaus B, et al. 2014. PLoS Pathog. 10:1003913. PubMed
  2. Verhagen J and Wraith DC. 2014. J. Immunol. Methods. S0022. (FC) PubMed
  3. Verhagen J, et al. 2014. PLoS One. 9e:108023. (FC) PubMed
Product Citations
  1. Arnold IC, et al. 2019. PLoS Pathog. 15:e1007866. PubMed
  2. Mishra A, et al. 2021. Cell. 184(13):3394-3409.e20. PubMed
RRID
AB_2561841 (BioLegend Cat. No. 145201)
AB_2561841 (BioLegend Cat. No. 145202)

Antigen Details

Structure
Type I transmembrane protein, 140 kD, 2 complement binding domains (CUB), 2 coagulation factor V/VIII homology domains (FV/FVIII), one meprin, A5, receptor tyrosine phosphatase domain (MAM)
Distribution

Natural regulatory T cells (nTreg), subset of invariant natural killer T cells (iNKT), endothelial cells, neurons

Function
Angiogenesis, axon guidance, modulation of the immune response
Ligand/Receptor
VEGF165, semaphorin-3A
Cell Type
Dendritic cells, Endothelial cells, Neurons, NKT cells, Tregs
Biology Area
Angiogenesis, Cancer Biomarkers, Cardiovascular Biology, Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Synaptic Biology
Molecular Family
Adaptor Proteins, Adhesion Molecules, CD Molecules
Antigen References

1. Yadav M, et al. 2012. J. Exp. Med. 209:1713.
2. Weiss JM, et al. 2012. J. Exp. Med. 209:1723.
3. Hansen W, et al. 2012. J. Exp. Med. 209:2001.
4. Milpied P, et al. 2011. Blood 118:2993.

Gene ID
18186 View all products for this Gene ID
UniProt
View information about CD304 on UniProt.org
Go To Top Version: 1    Revision Date: 02/13/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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