- Clone
- M5/114.15.2 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- MHC class II
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
107631 | 125 µL | $176 | ||||
107632 | 50 µg | $237 |
These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b,d,q,r bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Activated C57BL/6 mouse spleen cells
- Formulation
- µl size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
- Concentration
- µg sizes: 0.2 mg/mLµL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
IHC-F - VerifiedSB - Reported in the literature, not verified in house
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µl size, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
-
Violet Laser (405 nm)
- Application Notes
-
The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek MHC class II alloantigens from mice carrying H-2p,r,q,b,d,u haplotypes. Clone M5/114.15.2 however does not react wtih I-Af, I-Ak, or I-As MHC class II alloantigens.1
Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of frozen sections2,3,6, in vitro and in vivo blocking of antigen presentation or ligand binding4-7, and spatial biology (IBEX)17,18. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 107655 & 107656). - Additional Product Notes
-
Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. The method relies on cyclical bleaching of panels of fluorescent antibodies in order to image and analyze many markers over multiple cycles of staining, imaging, and, bleaching. It is a community-developed open-access method developed by the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).
-
Application References
(PubMed link indicates BioLegend citation) -
- Bhattacharya A, et al. 1981. J. Immunol. 127:2488. (IP)
- Viville S, et al. 1993. Cell 72:635. (IHC)
- Nelson AJ, et al. 1993. J. Immunol. 151:2453. (IHC)
- Shi Y, et al. 1998. J. Exp. Med. 187:367. (Block)
- Yamashita I, et al. 1993. Int. Immunol. 5:1139.
- Guo M, et al. 1995. Zygote 3:65. (IHC)
- Kim A, et al. 2004. Exp. Mol. Med. 36:428. (Block)
- Luckashenak NA, et al. 2006. J. Immunol. 177:5177.
- Venanzi ES, et al. 2007. J. Immunol. 179:5693.
- Christensen SR, et al. 2006. Immunity 25:417. PubMed
- Matte-Martone C, et al. 2008. Blood 111:3884. PubMed
- De Pascalis R, et al. 2008. Infect. Immun. 76:4311. PubMed
- Kuns RD, et al. 2009. Blood 113:5999. PubMed
- Sabatino JJ, et al. 2011. J. Exp. Med. 208:81. PubMed
- Draber P, et al. 2011. Mol Cell Biol. 22:4550. PubMed
- Fu H, et al. 2014. Nat Commun. 5:3436. PubMed
- Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
- Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
- Product Citations
-
- RRID
-
AB_10900075 (BioLegend Cat. No. 107631)
AB_10900075 (BioLegend Cat. No. 107632)
Antigen Details
- Structure
- MHC class II
- Distribution
-
B cell and activated T cells, APCs of the H-2b,d,q,r bearing mice
- Function
- Antigen presentation
- Ligand/Receptor
- CD3/TCR, CD4
- Cell Type
- Antigen-presenting cells, B cells, Dendritic cells, T cells, Tregs
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- MHC Antigens
- Antigen References
-
1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323. - Gene ID
- 14961 View all products for this Gene ID 14969 View all products for this Gene ID
- UniProt
- View information about I-A/I-E on UniProt.org
Related FAQs
- What is the F/P ratio range of our BV421™ format antibody reagents?
-
It is lot-specific. On average it ranges between 2-4.
- If an antibody clone has been previously successfully used in IBEX in one fluorescent format, will other antibody formats work as well?
-
It’s likely that other fluorophore conjugates to the same antibody clone will also be compatible with IBEX using the same sample fixation procedure. Ultimately a directly conjugated antibody’s utility in fluorescent imaging and IBEX may be specific to the sample and microscope being used in the experiment. Some antibody clone conjugates may perform better than others due to performance differences in non-specific binding, fluorophore brightness, and other biochemical properties unique to that conjugate.
- Will antibodies my lab is already using for fluorescent or chromogenic IHC work in IBEX?
-
Fundamentally, IBEX as a technique that works much in the same way as single antibody panels or single marker IF/IHC. If you’re already successfully using an antibody clone on a sample of interest, it is likely that clone will have utility in IBEX. It is expected some optimization and testing of different antibody fluorophore conjugates will be required to find a suitable format; however, legacy microscopy techniques like chromogenic IHC on fixed or frozen tissue is an excellent place to start looking for useful antibodies.
- Are other fluorophores compatible with IBEX?
-
Over 18 fluorescent formats have been screened for use in IBEX, however, it is likely that other fluorophores are able to be rapidly bleached in IBEX. If a fluorophore format is already suitable for your imaging platform it can be tested for compatibility in IBEX.
- The same antibody works in one tissue type but not another. What is happening?
-
Differences in tissue properties may impact both the ability of an antibody to bind its target specifically and impact the ability of a specific fluorophore conjugate to overcome the background fluorescent signal in a given tissue. Secondary stains, as well as testing multiple fluorescent conjugates of the same clone, may help to troubleshoot challenging targets or tissues. Using a reference control tissue may also give confidence in the specificity of your staining.
- How can I be sure the staining I’m seeing in my tissue is real?
-
In general, best practices for validating an antibody in traditional chromogenic or fluorescent IHC are applicable to IBEX. Please reference the Nature Methods review on antibody based multiplexed imaging for resources on validating antibodies for IBEX.
Other Formats
View All I-A/I-E Reagents Request Custom ConjugationCustomers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Biotin anti-mouse I-A/I-E
C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I... -
FITC anti-mouse I-A/I-E
C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I... -
PE anti-mouse I-A/I-E
C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I... -
Purified anti-mouse I-A/I-E
C57/B6 mouse splenocytes were stained with purified anti-mou... Fresh, frozen mouse spleen was stained with purified I-A/I-E... -
PE/Cyanine5 anti-mouse I-A/I-E
C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I... -
APC anti-mouse I-A/I-E
C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I... -
Alexa Fluor® 488 anti-mouse I-A/I-E
C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I... Paraformaldehyde-fixed (1%), 500 µm-thick mouse spleen secti... -
Alexa Fluor® 647 anti-mouse I-A/I-E
C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I... -
Pacific Blue™ anti-mouse I-A/I-E
C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I... -
Alexa Fluor® 700 anti-mouse I-A/I-E
C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I... Confocal image of C57BL/6 mouse spleen sample acquired using... Confocal image of C57BL/6 mouse thymus sample acquired using... Confocal image of C57BL/6 mouse lung sample acquired using t... Confocal image of C57BL/6 mouse small intestine sample acqui... Confocal image of C57BL/6 mouse liver sample acquired using ... -
PerCP/Cyanine5.5 anti-mouse I-A/I-E
C57BL/6 mouse splenocytes were stained with CD45R/B220 APC a... -
PerCP anti-mouse I-A/I-E
C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I... -
APC/Cyanine7 anti-mouse I-A/I-E
C57BL/6 mouse splenocytes were stained with anti-mouse CD11b... -
PE/Cyanine7 anti-mouse I-A/I-E
C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I... -
Brilliant Violet 421™ anti-mouse I-A/I-E
C57BL/6 mouse splenocytes were stained with mouse I-A/I-E (c... BL/6 mouse lymph nodes, fixed O/N in PLP, blocked with 10% r... Mice were injected subcutaneously with sheep red blood cells... -
Brilliant Violet 510™ anti-mouse I-A/I-E
C57BL/6 mouse splenocytes were stained with mouse I-A/I-E (c... -
Purified anti-mouse I-A/I-E (Maxpar® Ready)
C57BL/6 mouse bone marrow cells stained with 174Yb-anti-I-A/... -
Brilliant Violet 605™ anti-mouse I-A/I-E
C57BL/6 mouse splenocytes were stained with mouse I-A/I-E (c... -
Brilliant Violet 650™ anti-mouse I-A/I-E
C57 mouse splenocytes were stained with I-A/I-E (clone M5/11... -
Brilliant Violet 711™ anti-mouse I-A/I-E
C57 mouse splenocytes were stained with I-A/I-E (clone M5/11... -
Brilliant Violet 785™ anti-mouse I-A/I-E
C57 mouse splenocytes were stained with I-A/I-E (clone M5/11... -
PE/Dazzle™ 594 anti-mouse I-A/I-E
C57 mouse splenocytes were stained with I-A/I-E (clone M5/11... -
Alexa Fluor® 594 anti-mouse I-A/I-E
C57BL/6 mouse frozen spleen section was fixed with 4% parafo... Paraformaldehyde-fixed (4%), 500 μm-thick mouse spleen secti... -
APC/Fire™ 750 anti-mouse I-A/I-E
C57BL/6 mouse splenocytes were stained with mouse I-A/I-E (c... -
TotalSeq™-A0117 anti-mouse I-A/I-E
-
Ultra-LEAF™ Purified anti-mouse I-A/I-E
C57/B6 mouse splenocytes were stained with LEAF™ anti-mouse ... -
TotalSeq™-B0117 anti-mouse I-A/I-E
-
TotalSeq™-C0117 anti-mouse I-A/I-E
-
Spark Blue™ 550 anti-mouse I-A/I-E
C57BL/6 mouse splenocytes stained with I-A/I-E (clone M5/114... -
PE/Fire™ 640 anti-mouse I-A/I-E
C57BL/6 mouse splenocytes were stained with anti-mouse/human... -
Spark YG™ 581 anti-mouse I-A/I-E
C57BL/6 mouse splenocytes were stained with anti-mouse CD45R... -
PE/Fire™ 810 anti-mouse I-A/I-E
C57BL/6 mouse splenocytes were stained with anti-mouse CD45R... -
Spark UV™ 387 anti-mouse I-A/I-E
C57BL/6 mouse splenocytes were stained with anti-mouse CD45R... -
Spark Violet™ 538 anti-mouse I-A/I-E
C57BL/6 mouse splenocytes were stained with anti-mouse CD45... -
PerCP/Fire™ 806 anti-mouse I-A/I-E
C57BL/6 mouse splenocytes were stained with anti-mouse CD45R... -
Spark Red™ 718 anti-mouse I-A/I-E
C57BL/6 mouse splenocytes were stained with anti-mouse/human... -
APC/Fire™ 810 anti-mouse I-A/I-E
C57BL/6 mouse splenocytes were stained with anti-mouse/human... -
Spark PLUS UV395™ anti-mouse I-A/I-E
C57BL/6 mouse splenocytes were stained with anti-mouse CD45R...
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