- Clone
- M5/114.15.2 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- MHC class II
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
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107637 | 100 µg | $124 |
These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b,d,q,r bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Activated C57BL/6 mouse spleen cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1.0 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
CyTOF® - Verified - Recommended Usage
-
This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.
- Application Notes
-
The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek MHC class II alloantigens from mice carrying H-2p,r,q,b,d,u haplotypes. Clone M5/114.15.2 however does not react wtih I-Af, I-Ak, or I-As MHC class II alloantigens.1
Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of frozen sections2,3,6, in vitro and in vivo blocking of antigen presentation or ligand binding4-7, and spatial biology (IBEX)17,18. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 107655 & 107656). - Additional Product Notes
-
Maxpar® is a registered trademark of Standard BioTools Inc.
-
Application References
(PubMed link indicates BioLegend citation) -
- Bhattacharya A, et al. 1981. J. Immunol. 127:2488. (IP)
- Viville S, et al. 1993. Cell 72:635. (IHC)
- Nelson AJ, et al. 1993. J. Immunol. 151:2453. (IHC)
- Shi Y, et al. 1998. J. Exp. Med. 187:367. (Block)
- Yamashita I, et al. 1993. Int. Immunol. 5:1139.
- Guo M, et al. 1995. Zygote 3:65. (IHC)
- Kim A, et al. 2004. Exp. Mol. Med. 36:428. (Block)
- Luckashenak NA, et al. 2006. J. Immunol. 177:5177.
- Venanzi ES, et al. 2007. J. Immunol. 179:5693.
- Christensen SR, et al. 2006. Immunity 25:417. PubMed
- Matte-Martone C, et al. 2008. Blood 111:3884. PubMed
- De Pascalis R, et al. 2008. Infect. Immun. 76:4311. PubMed
- Kuns RD, et al. 2009. Blood 113:5999. PubMed
- Sabatino JJ, et al. 2011. J. Exp. Med. 208:81. PubMed
- Draber P, et al. 2011. Mol Cell Biol. 22:4550. PubMed
- Fu H, et al. 2014. Nat Commun. 5:3436. PubMed
- Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
- Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
- Product Citations
-
- RRID
-
AB_2563771 (BioLegend Cat. No. 107637)
Antigen Details
- Structure
- MHC class II
- Distribution
-
B cell and activated T cells, APCs of the H-2b,d,q,r bearing mice
- Function
- Antigen presentation
- Ligand/Receptor
- CD3/TCR, CD4
- Cell Type
- Antigen-presenting cells, B cells, Dendritic cells, T cells, Tregs
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- MHC Antigens
- Antigen References
-
1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323. - Gene ID
- 14961 View all products for this Gene ID 14969 View all products for this Gene ID
- UniProt
- View information about I-A/I-E on UniProt.org
Related FAQs
- Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?
-
We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm®. Please contact Fluidigm® directly for additional data and further details.
- Can I use Maxpar® Ready format clones for flow cytometry staining?
-
We have not tested the Maxpar® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.
- I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.
-
We only supply the antibody and not test that in house. Please contact Fluidigm® directly for troubleshooting advice: http://techsupport.fluidigm.com/
- Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?
-
The Maxpar® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.
Other Formats
View All I-A/I-E Reagents Request Custom ConjugationCustomers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Biotin anti-mouse I-A/I-E
C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I... -
FITC anti-mouse I-A/I-E
C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I... -
PE anti-mouse I-A/I-E
C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I... -
Purified anti-mouse I-A/I-E
C57/B6 mouse splenocytes were stained with purified anti-mou... Fresh, frozen mouse spleen was stained with purified I-A/I-E... -
PE/Cyanine5 anti-mouse I-A/I-E
C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I... -
APC anti-mouse I-A/I-E
C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I... -
Alexa Fluor® 488 anti-mouse I-A/I-E
C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I... Paraformaldehyde-fixed (1%), 500 µm-thick mouse spleen secti... -
Alexa Fluor® 647 anti-mouse I-A/I-E
C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I... -
Pacific Blue™ anti-mouse I-A/I-E
C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I... -
Alexa Fluor® 700 anti-mouse I-A/I-E
C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I... Confocal image of C57BL/6 mouse spleen sample acquired using... Confocal image of C57BL/6 mouse thymus sample acquired using... Confocal image of C57BL/6 mouse lung sample acquired using t... Confocal image of C57BL/6 mouse small intestine sample acqui... Confocal image of C57BL/6 mouse liver sample acquired using ... -
PerCP/Cyanine5.5 anti-mouse I-A/I-E
C57BL/6 mouse splenocytes were stained with CD45R/B220 APC a... -
PerCP anti-mouse I-A/I-E
C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I... -
APC/Cyanine7 anti-mouse I-A/I-E
C57BL/6 mouse splenocytes were stained with anti-mouse CD11b... -
PE/Cyanine7 anti-mouse I-A/I-E
C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I... -
Brilliant Violet 421™ anti-mouse I-A/I-E
C57BL/6 mouse splenocytes were stained with mouse I-A/I-E (c... BL/6 mouse lymph nodes, fixed O/N in PLP, blocked with 10% r... Mice were injected subcutaneously with sheep red blood cells... -
Brilliant Violet 510™ anti-mouse I-A/I-E
C57BL/6 mouse splenocytes were stained with mouse I-A/I-E (c... -
Purified anti-mouse I-A/I-E (Maxpar® Ready)
C57BL/6 mouse bone marrow cells stained with 174Yb-anti-I-A/... -
Brilliant Violet 605™ anti-mouse I-A/I-E
C57BL/6 mouse splenocytes were stained with mouse I-A/I-E (c... -
Brilliant Violet 650™ anti-mouse I-A/I-E
C57 mouse splenocytes were stained with I-A/I-E (clone M5/11... -
Brilliant Violet 711™ anti-mouse I-A/I-E
C57 mouse splenocytes were stained with I-A/I-E (clone M5/11... -
Brilliant Violet 785™ anti-mouse I-A/I-E
C57 mouse splenocytes were stained with I-A/I-E (clone M5/11... -
PE/Dazzle™ 594 anti-mouse I-A/I-E
C57 mouse splenocytes were stained with I-A/I-E (clone M5/11... -
Alexa Fluor® 594 anti-mouse I-A/I-E
C57BL/6 mouse frozen spleen section was fixed with 4% parafo... Paraformaldehyde-fixed (4%), 500 μm-thick mouse spleen secti... -
APC/Fire™ 750 anti-mouse I-A/I-E
C57BL/6 mouse splenocytes were stained with mouse I-A/I-E (c... -
TotalSeq™-A0117 anti-mouse I-A/I-E
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Ultra-LEAF™ Purified anti-mouse I-A/I-E
C57/B6 mouse splenocytes were stained with LEAF™ anti-mouse ... -
TotalSeq™-B0117 anti-mouse I-A/I-E
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TotalSeq™-C0117 anti-mouse I-A/I-E
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Spark Blue™ 550 anti-mouse I-A/I-E
C57BL/6 mouse splenocytes stained with I-A/I-E (clone M5/114... -
PE/Fire™ 640 anti-mouse I-A/I-E
C57BL/6 mouse splenocytes were stained with anti-mouse/human... -
Spark YG™ 581 anti-mouse I-A/I-E
C57BL/6 mouse splenocytes were stained with anti-mouse CD45R... -
PE/Fire™ 810 anti-mouse I-A/I-E
C57BL/6 mouse splenocytes were stained with anti-mouse CD45R... -
Spark UV™ 387 anti-mouse I-A/I-E
C57BL/6 mouse splenocytes were stained with anti-mouse CD45R... -
Spark Violet™ 538 anti-mouse I-A/I-E
C57BL/6 mouse splenocytes were stained with anti-mouse CD45... -
PerCP/Fire™ 806 anti-mouse I-A/I-E
C57BL/6 mouse splenocytes were stained with anti-mouse CD45R... -
Spark Red™ 718 anti-mouse I-A/I-E
C57BL/6 mouse splenocytes were stained with anti-mouse/human... -
APC/Fire™ 810 anti-mouse I-A/I-E
C57BL/6 mouse splenocytes were stained with anti-mouse/human... -
Spark PLUS UV395™ anti-mouse I-A/I-E
C57BL/6 mouse splenocytes were stained with anti-mouse CD45R...
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