- Clone
- P84 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- SHPS-1, BIT, P84, PTPNS1, CD172 antigen-like family member A
- Isotype
- Rat IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
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144032 | 50 µg | $316 |
CD172a, also known as SIRPα, is a type I transmembrane protein with one V-set Ig-like and two C-set Ig-like domains in the extracellular portion, and two ITIM motifs and a proline-rich region in the cytoplasmic tail. CD172a is expressed by monocytes, macrophages, myeloid cells, and neuronal tissue. The phosphorylation of SIRPα ITIMs induces the recruitment and activation of the tyrosine phosphatases PTPN6 and PTPN11, resulting in the negative regulation of several biological processes. The ligands of CD172a are CD47, SP-A, and SP-D.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse brain membrane protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510™ under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 510™ excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
-
Violet Laser (405 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: blocking SIRPa interaction with CD474, in vivo blocking of dendritic cell migration3, enhancing of macrophage phagocytosis2,4, immunohistochemical staining of cerebellum frozen sections1, immunoprecipitation2,4, and spatial biology (IBEX)5,6.
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Application References
(PubMed link indicates BioLegend citation) -
- Comu S, et al. 1997. J. Neurosci. 17:8702. (IHC)
- Gresham HD, et al. 2000. J. Exp. Med. 191:515. (IP)
- Fukunaga A, et al. 2004. J. Immunol. 172:4091. (Block)
- Oldenborg PA, et al. 2000. Science 288:2051. (Block, IP)
- Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
- Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
- Product Citations
-
- RRID
-
AB_2810411 (BioLegend Cat. No. 144032)
Antigen Details
- Structure
- Type I transmembrane protein
- Distribution
-
Monocytes, macrophages, myeloid cells, neuronal tissue
- Function
- Negative regulation of several biological processes
- Interaction
- PTPN6, PTPN11
- Ligand/Receptor
- CD47, SP-A, SP-D
- Cell Type
- Dendritic cells, Macrophages, Monocytes, Neutrophils
- Biology Area
- Cell Adhesion, Cell Biology, Immunology, Signal Transduction
- Molecular Family
- Adhesion Molecules, CD Molecules, Protein Kinases/Phosphatase
- Antigen References
-
1. Zhao XW, et al. 2011. P. Natl. Acad. Sci. USA 108:18342.
2. Verjan-Garcia N, et al. 2011. J. Immunol. 187:2268.
3. Sato-Hashimoto M, et al. 2011. J. Immunol. 187:291.
4. Raymond M, et al. 2010. Eur. J. Immunol. 40:3510. - Gene ID
- 19261 View all products for this Gene ID
- UniProt
- View information about CD172alpha on UniProt.org
Related Pages & Pathways
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Other Formats
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse CD172a (SIRPα)
C57BL/6 mouse bone marrow cells were stained with CD11b APC ... -
FITC anti-mouse CD172a (SIRPα)
C57BL/6 mouse bone marrow cells were stained with CD11b APC ... Fixed whole mount mouse spleen was stained with FITC CD172a ... -
PE/Cyanine7 anti-mouse CD172a (SIRPα)
C57BL/6 mouse bone marrow cells were stained with CD11b FITC... -
PerCP/Cyanine5.5 anti-mouse CD172a (SIRPα)
C57BL/6 mouse bone marrow cells were stained with CD11b APC ... -
PE anti-mouse CD172a (SIRPα)
C57BL/6 mouse bone marrow cells were stained with CD11b FITC... -
APC anti-mouse CD172a (SIRPα)
C57BL/6 mouse bone marrow cells were stained with CD11b FITC... -
PE/Dazzle™ 594 anti-mouse CD172a (SIRPα)
C57BL/6 mouse bone marrow cells were stained with CD11b APC ... -
Alexa Fluor® 594 anti-mouse CD172a (SIRPα)
C57BL/6 mouse frozen spleen section was fixed with 4% parafo... Paraformaldehyde-fixed (4%), 500 μm-thick mouse spleen secti... -
APC/Cyanine7 anti-mouse CD172a (SIRPα)
C57BL/6 mouse bone marrow cells were stained with CD11b FITC... -
Alexa Fluor® 488 anti-mouse CD172a (SIRPα)
C57BL/6 mouse bone marrow cells were stained with CD11b PE a... Confocal image of C57BL/6 mouse thymus sample acquired using... Mice were injected subcutaneously with sheep red blood cells... -
Alexa Fluor® 700 anti-mouse CD172a (SIRPα)
C57BL/6 mouse bone marrow cells were stained with CD11b FITC... -
Biotin anti-mouse CD172a (SIRPα)
C57BL/6 mouse bone marrow cells were stained with CD11b PE a... -
Alexa Fluor® 647 anti-mouse CD172a (SIRPα)
C57BL/6 mouse bone marrow cells were stained with CD11b PE a... Paraformaldehyde-fixed (4%), 500 μm-thick mouse spleen secti... -
APC/Fire™ 750 anti-mouse CD172a (SIRPα)
C57BL/6 mouse bone marrow cells were stained with CD11b PE a... -
TotalSeq™-A0422 anti-mouse CD172a (SIRPα)
-
Brilliant Violet 510™ anti-mouse CD172a (SIRPα)
C57BL/6 mouse bone marrow cells were stained with CD11b APC ... -
TotalSeq™-C0422 anti-mouse CD172a (SIRPα)
-
Ultra-LEAF™ Purified anti-mouse CD172a (SIRPα)
C57BL/6 mouse bone marrow cells were stained with CD11b APC ... -
TotalSeq™-B0422 anti-mouse CD172a (SIRPα)
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PerCP/Fire™ 780 anti-mouse CD172a (SIRPα)
C57BL/6 mouse bone marrow cells were stained with anti-mouse... -
Spark Red™ 718 anti-mouse CD172a (SIRPα) (Flexi-Fluor™)
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