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Brilliant Violet 605™ anti-mouse CD183 (CXCR3) Antibody

Pricing & Availability
Clone
CXCR3-173 (See other available formats)
Regulatory Status
RUO
Other Names
CXCR3, G protein-coupled receptor 9 (GPR9), CKR-L2, IP10 receptor (IP10-R), Mig receptor (Mig-R )
Isotype
Armenian Hamster IgG
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Product Citations
publications
CXCR3-173_BV605_CXCR3_Antibody_FC_1_051413
  • CXCR3-173_BV605_CXCR3_Antibody_FC_1_051413
  • CXCR3-173_BV605_CXCR3_Antibody_FC_2_051413
Compare all formats See Brilliant Violet 605™ spectral data
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126523 125 µL $237
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Description

CD183, also known as CXCR3, is a member of the C-X-C chemokine family, characterized by a pair of cysteine residues separated by a single amino acid. CXCR3 is a 38 kD seven pass transmembrane receptor coupled to G-protein. It mediates Ca2+ mobilization and chemotaxis in response to C-X-C chemokines, such as IP10 (CXCL10), MIG (CXCL9), I-TAC (CXCL11) and PF4 (CXCL4). CXCR3 is expressed primarily on activiated T lymphocytes, NK cells, and some epithelial cells and endothelial cells. It is not expressed on B cells, monocytes, or granulocytes.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse N-terminus of CXCR3
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

From in-house testing, we observed improved staining resolution after adjusting the antibody incubation time to 45 minutes at room temperature for the Brilliant Violet 605™ conjugate. Staining using other conjugated formats may also benefit from longer incubation time.

Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Clone CXCR3-173 does not work for Western blotting.7 For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 126526) with endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered.

Application References

(PubMed link indicates BioLegend citation)
  1. Krug A, et al. 2002. J. Immunol. 169:6079.
  2. McAleer JP, et al. 2009. J. Immunol. 182:5322. PubMed
  3. Sharma R. 2009. J. Immunol. 183:3212. (FC) PubMed
  4. Barbi J, et al. 2009. FASEB J. 23:3990. PubMed
  5. Kimpfler S, et al. 2009. J. Immunol. 183:6330. PubMed
  6. Dürr C, et al. 2010. Cancer Res. 70:10170. (Block) PubMed
  7. Uppaluri R, et al. 2008. Transplantation 86:137.
Product Citations
  1. Lin YH 2023. Immunity. 56(1):207-223.e8. PubMed
  2. Pfenninger P, et al. 2022. Front Immunol. 13:777113. PubMed
  3. Blagih J, et al. 2020. Cell Rep. 30:481. PubMed
  4. Riedel R, et al. 2020. Nat Commun. 11:2570. PubMed
  5. Teng F, et al. 2021. Cell Rep. 37:110051. PubMed
RRID
AB_2561353 (BioLegend Cat. No. 126523)

Antigen Details

Distribution

T cells, plasmacytoid dendritic cells, GM-CSF activated CD34+ hematopoietic progenitors

Function
Essential in T cell recruitment to sites of inflammation
Cell Type
Dendritic cells, Hematopoietic stem and progenitors, T cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, GPCR
Gene ID
12766 View all products for this Gene ID
UniProt
View information about CD183 on UniProt.org
Go To Top Version: 2    Revision Date: 06/25/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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