- Clone
- HI100 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- IV N906
- Other Names
- GP180, L-CA, LCA, LY5, T200, PTPRC
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
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304107 | 25 tests | $44 | ||||
304108 | 100 tests | $105 |
CD45RA is a 205-220 kD single chain type I glycoprotein. It is an exon 4 splice variant of the tyrosine phosphatase CD45. The CD45RA isoform is expressed on resting/naïve T cells, medullary thymocytes, B cells and monocytes. CD45RA enhances both T cell receptor and B cell receptor signaling. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4. CD45 has also been reported to bind galectin-1. CD45 isoform expression can change in response to cytokines.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- Chimpanzee
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
SB - Community Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
Additional reported applications (for relevant formats of this clone) include: inhibition of CD45 functions2, immunohistochemical staining of frozen tissue sections3 and formalin-fixed paraffin-embedded tissue sections4, and immunocytochemistry15,16.
- Additional Product Notes
-
For the use of this antibody in spatial biology (SB), we have partnered with Bruker Spatial Biology Biosciences for demonstration of this antibody on their next-generation ChipCytometry instrument called the CellScape™. The CellScape platform is an end-to-end solution for highly multiplexed spatial omics. Combining an advanced, purpose-built imaging system with easy-to-use fluidics for walk-away automation, the CellScape system will accelerate your exploration into the rapidly evolving field of spatial biology. More information on the the Bruker Spatial Biology CellScape and a complete list of our antibodies that have been demonstrated on the instrument can be found here.
-
Application References
(PubMed link indicates BioLegend citation) -
- Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
- Yamada T, et al. 2002. J. Biol. Chem. 277:28830. (WB, Block)
- Weninger W, et al. 2003 J. Immunol. 170:4638. (IHC-F)
- Imanguli MM, et al. 2009. Blood. 113:3620 (IHC-P)
- Roque S, et al. 2007. J. Immunol. 178:8028. (FC) PubMed
- Smeltz RB. 2007. J. Immunol. 178:4786. (FC) PubMed
- Palendira U, et al. 2008. Blood (FC) PubMed
- Kuttruff S, et al. 2009. Blood 113:358. (FC) PubMed
- Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
- Alanio C, et al. 2010. Blood 115:3718. (FC) PubMed
- Iannello A, et al. 2010. J. Immunol. 184:114. (FC) PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Guereau-de-Arellan M, et al. 2011. Brain. 134:3578. PubMed
- Canque B, et al. 2000. Blood 96:3748. (ICC)
- Imanguli MM, et al. 2009. Blood 13:3620. (ICC)
- Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
- Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
- Product Citations
-
- RRID
-
AB_314411 (BioLegend Cat. No. 304107)
AB_314411 (BioLegend Cat. No. 304108)
Antigen Details
- Structure
- Tyrosine phosphatases, type I transmembrane (exon 4 splicing of CD45 gene), 205-220 kD
- Distribution
-
B cells, naïve T cells, monocytes
- Function
- Enhances TCR and BCR signaling
- Ligand/Receptor
- Galectin-1, CD2, CD3, CD4
- Cell Type
- B cells, Monocytes, T cells, Tregs
- Biology Area
- Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules
- Antigen References
-
1. Thomas M. 1989. Annu. Rev. Immunol. 7:339.
2. Trowbridge I, et al. 1994. Annu. Rev. Immunol.12:85. - Gene ID
- 5788 View all products for this Gene ID
- UniProt
- View information about CD45RA on UniProt.org
Related FAQs
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
- If an antibody clone has been previously successfully used in IBEX in one fluorescent format, will other antibody formats work as well?
-
It’s likely that other fluorophore conjugates to the same antibody clone will also be compatible with IBEX using the same sample fixation procedure. Ultimately a directly conjugated antibody’s utility in fluorescent imaging and IBEX may be specific to the sample and microscope being used in the experiment. Some antibody clone conjugates may perform better than others due to performance differences in non-specific binding, fluorophore brightness, and other biochemical properties unique to that conjugate.
- Will antibodies my lab is already using for fluorescent or chromogenic IHC work in IBEX?
-
Fundamentally, IBEX as a technique that works much in the same way as single antibody panels or single marker IF/IHC. If you’re already successfully using an antibody clone on a sample of interest, it is likely that clone will have utility in IBEX. It is expected some optimization and testing of different antibody fluorophore conjugates will be required to find a suitable format; however, legacy microscopy techniques like chromogenic IHC on fixed or frozen tissue is an excellent place to start looking for useful antibodies.
- Are other fluorophores compatible with IBEX?
-
Over 18 fluorescent formats have been screened for use in IBEX, however, it is likely that other fluorophores are able to be rapidly bleached in IBEX. If a fluorophore format is already suitable for your imaging platform it can be tested for compatibility in IBEX.
- The same antibody works in one tissue type but not another. What is happening?
-
Differences in tissue properties may impact both the ability of an antibody to bind its target specifically and impact the ability of a specific fluorophore conjugate to overcome the background fluorescent signal in a given tissue. Secondary stains, as well as testing multiple fluorescent conjugates of the same clone, may help to troubleshoot challenging targets or tissues. Using a reference control tissue may also give confidence in the specificity of your staining.
- How can I be sure the staining I’m seeing in my tissue is real?
-
In general, best practices for validating an antibody in traditional chromogenic or fluorescent IHC are applicable to IBEX. Please reference the Nature Methods review on antibody based multiplexed imaging for resources on validating antibodies for IBEX.
Other Formats
View All CD45RA Reagents Request Custom ConjugationCustomers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC anti-human CD45RA
Human peripheral blood lymphocytes stained with HI100 APC -
Biotin anti-human CD45RA
Human peripheral blood lymphocytes stained with biotinylated... -
FITC anti-human CD45RA
Human peripheral blood lymphocytes stained with HI100 FITC -
PE anti-human CD45RA
Human peripheral blood lymphocytes were stained with CD45RA ... Pre-lysed human blood leukocytes were stained with CD45RA (c... Multiplexed IHC staining of PE anti-CD45RA (clone HI100) on ... -
PE/Cyanine5 anti-human CD45RA
Human peripheral blood lymphocytes stained with HI100 PE/Cya... -
Purified anti-human CD45RA
Human peripheral blood platelets stained with purified HI100... SeqIF™ (sequential immunofluorescence) staining on COMET™ of... -
Alexa Fluor® 488 anti-human CD45RA
Human peripheral blood lymphocytes stained with HI100 Alexa ... -
Alexa Fluor® 647 anti-human CD45RA
Human peripheral blood lymphocytes were stained with CD45RA ... Human paraffin-embedded tonsil tissue slices were prepared w... -
Pacific Blue™ anti-human CD45RA
Human peripheral blood lymphocytes stained with HI100 Pacifi... -
Alexa Fluor® 700 anti-human CD45RA
Human peripheral blood lymphocytes stained with HI100 Alexa ... -
PerCP/Cyanine5.5 anti-human CD45RA
Human peripheral blood lymphocytes were stained with CD45RO ... Multiplexed IHC staining of PerCP/Cyanine5.5 anti-CD45RA (cl... -
PE/Cyanine7 anti-human CD45RA
Human peripheral blood lymphocytes were stained with HI100 P... -
APC/Cyanine7 anti-human CD45RA
Human peripheral blood lymphocytes were stained CD45RA (clon... -
Brilliant Violet 421™ anti-human CD45RA
Human peripheral blood lymphocytes were stained with CD45RO ... Human paraffin-embedded tonsil tissue slices were prepared w... -
Brilliant Violet 570™ anti-human CD45RA
Human peripheral blood lymphocytes were stained with CD45RO ... -
Brilliant Violet 605™ anti-human CD45RA
Human peripheral blood lymphocytes were stained with CD45RO ... -
Brilliant Violet 650™ anti-human CD45RA
Human peripheral blood lymphocytes were stained with CD45RO ... -
Brilliant Violet 711™ anti-human CD45RA
Human peripheral blood lymphocytes stained with CD45RA (clon... -
Brilliant Violet 785™ anti-human CD45RA
Human peripheral blood lymphocytes stained with CD45RA (clon... -
Brilliant Violet 510™ anti-human CD45RA
Human peripheral blood lymphocytes were stained with CD45RO ... -
Purified anti-human CD45RA (Maxpar® Ready)
Human PBMCs stained with 149Sm-anti-CD45RO (UCHL1) and 143Nd... -
PE/Dazzle™ 594 anti-human CD45RA
Human peripheral blood granulocytes were stained with CD45RA... -
APC/Fire™ 750 anti-human CD45RA
Human peripheral blood lymphocytes were stained with CD45RO ... -
PerCP anti-human CD45RA
Human peripheral blood lymphocytes were stained with CD45RA ... -
FITC anti-human CD45RA
Typical results from human peripheral blood lymphocytes stai... -
TotalSeq™-A0063 anti-human CD45RA
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Alexa Fluor® 594 anti-human CD45RA
Human paraffin-embedded tonsil tissue slices were prepared w... -
TotalSeq™-B0063 anti-human CD45RA
-
TotalSeq™-C0063 anti-human CD45RA
-
Brilliant Violet 750™ anti-human CD45RA
Human peripheral blood lymphocytes were stained with CD45RO ... -
Spark NIR™ 685 anti-human CD45RA
Human peripheral blood lymphocytes were stained with CD45RO ... -
APC anti-human CD45RA
Typical results from human peripheral blood lymphocytes stai... -
PE/Fire™ 640 anti-human CD45RA
Human peripheral blood lymphocytes were stained with anti-hu... -
PE/Fire™ 700 anti-human CD45RA Antibody
Human peripheral blood lymphocytes were stained with anti-hu... -
Spark YG™ 581 anti-human CD45RA
Human peripheral blood lymphocytes were stained with anti-hu... -
TotalSeq™-D0063 anti-human CD45RA
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Spark Violet™ 423 anti-human CD45RA
Human peripheral blood lymphocytes were stained with anti-hu... IHC staining of Spark Violet™ 423 anti-human CD45RA (clone H... -
GMP FITC anti-human CD45RA
Typical results from human peripheral blood lymphocytes stai... -
PE/Cyanine7 anti-human CD45RA
Typical results from human peripheral blood lymphocytes stai... -
PE/Dazzle™ 594 anti-human CD45RA
Typical results from human peripheral blood lymphocytes stai... -
APC/Fire™ 750 anti-human CD45RA
Typical results from human peripheral blood lymphocytes stai... -
Spark UV™ 387 anti-human CD45RA
Human peripheral blood lymphocytes were stained with anti-hu... -
GMP APC anti-human CD45RA
Typical results from human peripheral blood lymphocytes stai... -
TotalSeq™-Bn0063 anti-human CD45RA
-
Spark Blue™ 550 anti-human CD45RA
Human peripheral blood lymphocytes were stained with anti-hu... -
GMP PE/Dazzle™ 594 anti-human CD45RA
Typical results from human peripheral blood lymphocytes stai... -
GMP APC/Fire™ 750 anti-human CD45RA
Typical results from human peripheral blood lymphocytes stai... -
Spark PLUS UV395™ anti-human CD45RA
Human peripheral blood lymphocytes were stained with anti-hu... -
Spark Red™ 718 anti-human CD45RA
Human peripheral blood lymphocytes were stained with anti-hu... -
APC/Fire™ 810 anti-human CD45RA
Human peripheral blood lymphocytes were stained with anti-hu... -
PE/Fire™ 810 anti-human CD45RA
Human peripheral blood lymphocytes were stained with anti-hu... -
Spark Blue™ 574 anti-human CD45RA (Flexi-Fluor™)
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