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FITC anti-human CD51 Antibody

Pricing & Availability
Clone
NKI-M9 (See other available formats)
Regulatory Status
RUO
Workshop
IV 103
Other Names
Vitronectin receptor α chain, Integrin αV, αV integrin, ITGAV
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
NKI-M9_FITC_020808
Human melanoma cell line M21 stained with NKI-M9 FITC
  • NKI-M9_FITC_020808
    Human melanoma cell line M21 stained with NKI-M9 FITC
Compare all formats See FITC spectral data
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327907 25 tests $94
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327908 100 tests $218
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Description

CD51 is a type I integral membrane glycoprotein, known as vitronectin receptor α chain, or integrin αV. It forms heterodimer with integrin β1 (CD29), β3 (CD61), β5, β6, or β8. CD51 contains two disulfide-linked subunits of 125 kD and 24 kD, and is expressed on endothelial cells, fibroblasts, macrophages, platelets, osteoclasts, neuroblastoma, melanoma, and hepatoma cells. Many extracellular matrix proteins with RGD-motifs are CD51 ligands. In association with its β chains, CD51 binds vitronectin, von Willebrand factor, fibronectin, thrombospondin, osteopontin, fibrinogen, and laminin. CD51, as an adhesion molecule, plays important roles in leukocytes homing and rolling, mediates bone absorption and angiogenesis.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Melonama cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Defilippi P, et al. 1991. J. Cell Biol. 114:855. PubMed
  3. Burdick MM, et al. 2003. Am. J. Physiol.Cell Ph. 284:C977. PubMed
  4. Grzeszkiewicz TM, et al. 2001. J. Biol. Chem. 276:21943.
  5. Sonnenberg A, et al. 1990. J. Cell Biol. 110:2145. PubMed
  6. Silverman AP, et al. 2008. J Mol Biol. 385:1064. PubMed
  7. Khurana S, et al. 2013. Blood. 121:2587. PubMed
Product Citations
  1. Silverman A, et al. 2008. J Mol Biol. 385:1064. PubMed
  2. Arulmoli J, et al. 2016. Acta Biomater. 43:122-138. PubMed
  3. Wang J, et al. 2016. Oncogene. 10.1038/onc.2016.299. PubMed
  4. Lawrence M, et al. 2022. Sci Adv. 8:eabj8618. PubMed
  5. Kenswil KJG, et al. 2021. Cell Stem Cell. 28(4):653-670.e11. PubMed
  6. Goncharova V, et al. 2014. Stem Cells Trans Med. 3:229. PubMed
  7. Sui X, et al. 2018. Cell Death Dis. 9:523. PubMed
  8. Shlamkovich T, et al. 2020. BMC Biology. 16(1):92. PubMed
  9. Fuhrmann A, et al. 2017. Biophys J. 112(4):736-745. PubMed
  10. Khurana S, et al. 2013. Blood. 121:2587. PubMed
  11. Malenica I, et al. 2021. Nat Commun. 12:5209. PubMed
RRID
AB_940558 (BioLegend Cat. No. 327907)
AB_940558 (BioLegend Cat. No. 327908)

Antigen Details

Structure
Integrin, forms heterodimer with integrin β1 (CD29), β3 (CD61), β5, β6, or β8, 125 kD and 24 kD in reduced condition, 150 kD (unreduced)
Distribution

Endothelial cells, fibroblasts, macrophages, platelets, osteoclasts, neuroblastoma, melanoma, and hepatoma cells.

Function
Adhesion, angiogenesis
Ligand/Receptor
Vitronectin, von Willebrand factor, fibrinogen, thrombospondin, osteopontin, denatured collagen, fibronectin, laminin.
Cell Type
Endothelial cells, Fibroblasts, Macrophages, Mesenchymal Stem Cells, Osteoclasts, Platelets
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Nesbitt S, et al. 1993. J. Biol. Chem. 268:16737.
2. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers Wiley-Liss A John Wiley & Sons Inc, Publication

Gene ID
3685 View all products for this Gene ID
UniProt
View information about CD51 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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