- Clone
- OX-62 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Integrin αE, integrin E, integrin αIEL, integrin αOX-62, Itgae
- Isotype
- Mouse IgG1
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
205505 | 100 µg | $153 |
CD103, also known as Integrin αE or integrin αIEL, is a single pass transmembrane protein composed by two chains, heavy and light, linked by a disulfide bond. It is expressed in the cell surface as an αE/β7 heterodimer. CD103 mediates adhesion of intraepithelial T cells (IEL) to the epithelium. CD103 is expressed by IEL, effector and memory Tregs, and dendritic cells and their immediate precursors. E-cadherin is the ligand for CD103.
Product DetailsProduct Details
- Verified Reactivity
- Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Density gradient enriched lymph veiled cells from PVG rats
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Blue Laser (488 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen skin and intestine epithelium1,4, immunocytochemical staining of cytospin preparations4, and Western blot1.
-
Application References
(PubMed link indicates BioLegend citation) -
- Brenan M, et al. 1992. J. Exp. Med. 175:1457. (IHC, WB)
- Chen-Woan M, et al. 1995. J. Immunol. Methods 178:157.
- Chen-Woan M, et al. 1996. J. Leuk. Biol. 59:196.
- Brenan M and Rees DJ. 2000. Eur. J. Immunol. 30:1527. (IHC)
- Product Citations
-
- RRID
-
AB_10680460 (BioLegend Cat. No. 205505)
Antigen Details
- Structure
- Single pass transmembrane protein, composed by two chains, heavy and light, linked by a disulfide bond. Associates with integrin β7 to form the αE/β7 complex.
- Distribution
-
Intraepithelial T lymphocytes, effector and memory Tregs, dendritic cells and their immediate precursors.
- Function
- Mediates adhesion of intraepithelial T cells (IEL) to epithelium.
- Ligand/Receptor
- E-cadherin
- Cell Type
- Dendritic cells, T cells, Tregs
- Biology Area
- Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Neuroscience, Synaptic Biology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Varas A, et al. 1998. Blood 92:93.
2. Brissette-Storkus CS, et al. 2002. J. Leukoc. Biol. 71:941.
3. Hubert FX, et al. 2004. J. Immunol. 172:7485.
4. Tsuchihashi S, et al. 2006. J. Immunol. 176:616.
5. Lührmann A, et al. 2007. Immunol. Lett. 108:167. - Gene ID
- 83577 View all products for this Gene ID
- UniProt
- View information about CD103 on UniProt.org
Related FAQs
Other Formats
View All CD103 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
FITC anti-rat CD103 (αE Integrin) | OX-62 | FC |
Alexa Fluor® 647 anti-rat CD103 (αE Integrin) | OX-62 | FC |
Biotin anti-rat CD103 (αE Integrin) | OX-62 | FC |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
FITC anti-rat CD103 (αE Integrin)
LOU rat intestinal intraepithelial lymphocytes were stained ... -
Alexa Fluor® 647 anti-rat CD103 (αE Integrin)
LOU rat intestinal intraepithelial lymphocytes were stained ... -
Biotin anti-rat CD103 (αE Integrin)
LOU rat intestinal intraepithelial lymphocytes were stained ...
Follow Us