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FITC anti-rat CD49d Antibody

Pricing & Availability
Clone
MRα4-1 (See other available formats)
Regulatory Status
RUO
Other Names
Integrin α4 chain, α4 integrin, VLA-4 α chain, ITGA4
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
MRα4-1
Lou rat blood lymphocytes stained with MRα4-1 FITC
  • MRα4-1
    Lou rat blood lymphocytes stained with MRα4-1 FITC
See FITC spectral data
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200103 50 µg $101
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Description

CD49d is a 150 kD glycoprotein, also known as integrin α4 or VLA-4 α chain. It is expressed as a heterodimer with either of two β chains, β1 or β7. The α4β1 integrin (also known as VLA-4, CD49d/CD29) is expressed on peripheral T and B lymphocytes, thymocytes, and monocytes, while the α4β7 integrin (also known as LPAM-1) is expressed on peripheral lymphocytes. The α4β1 and α4β7 integrins mediate cell-cell and cell-matrix interactions by interacting with the ligands CD106 (VCAM-1), MAdCAM-1, and fibronectin. Soluble MRα4-1 antibody can partially inhibit in vitro binding of VCAM-1 and mast cells to fibronectin and inhibit mast cell degranulation. Immobilized MRα4-1 antibody can enhance mast cell degranulation in vitro.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
RBL-2H3 rat basophilic leukemia cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications include: immunoprecipitation1, in vitro activation, in vitro inhibition1 of binding of VCAM-1 and mast cells to fibronectin , and in vivo inhibition of VLA-4 functions1. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 200106).

Application References

(PubMed link indicates BioLegend citation)
  1. Yasuda M, et al. 1995. Int. Immunol. 7:251. (IP, Block)
Product Citations
  1. Greis C, et al. 2017. Crit Care. 10.1186/s13054-017-1654-9. PubMed
  2. Jiang M, et al. 2020. Exp Ther Med. 1.989583333. PubMed
  3. Felce JH, et al. 2018. Sci Signal. 11:eaat0756. PubMed
RRID
AB_2265193 (BioLegend Cat. No. 200103)

Antigen Details

Structure
Integrin α chain, 150 kD
Distribution

α4/β1- peripheral T cells and B cell lymphocytes, thymocytes, and monocytes; α4/β7- peripheral lymphocytes

Function
Adhesion
Ligand/Receptor
CD106 (VCAM-1), MAdCAM-1, fibronectin
Cell Type
B cells, Lymphocytes, Monocytes, T cells, Thymocytes
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Yasuda, M., et al. 1995. Int. Immunol. 7:251.
2. Springer, T.A. 1994. Cell 76:301.
3. Issekutz, T. B., et al. 1991. J. Immunol. 147:4178.
4. Issekutz, T. B., et al. 1991. J. Immunol. 147:109.
5. Yang, H., et al. 1995. Transplantation 60:71.
6. Palecanda, A., et al. 1997. J. Immunol. 158:2904.

Gene ID
311144 View all products for this Gene ID
UniProt
View information about CD49d on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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