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ELISA MAX™ Deluxe Set Human IL-6

Pricing & Availability
Regulatory Status
RUO
Other Names
IL-6 ELISA Kit
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Product Citations
publications
human il-6_122109
  • human il-6_122109
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430504 5 plates $305
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430515 10 Plates $586
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430516 20 Plates $938
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Description

ELISA Development Systems Engineered for Sensitive, Precise and Reliable Serum and Plasma Cytokine Quantitation

BioLegend's ELISA Max™ Sets provide all the time-savings, convenience and performance of a high-end, commercial ELISA kit for a fraction of the price!

BioLegend's ELISA Max™ Sets, in two appealing formats, address the need for sensitivity, accuracy, dependability, and versatility of an ELISA development system, while providing maximum cost effectiveness, value, and ease of use.

Both the ELISA Max™ Set Standard and ELISA Max™ Set Deluxe are specifically engineered for accurate recovery and measurement of analytes in complex fluids, such as serum and plasma (as well as activated cell cultures) without any interference by confounding serum factors, such as heterophilic antibody or rheumatoid factor.

Product Details
Technical data sheet

Kit Contents

Kit Contents
  • Human IL-6 ELISA MAX™ Capture Antibody (200X)
  • Human IL-6 ELISA MAX™ Detection Antibody (200X)
  • Human IL-6 Standard
  • Avidin-HRP (1000X)
  • Substrate Solution A
  • Substrate Solution B
  • Coating Buffer A (5X)
  • Assay Diluent A (5X)

Product Details

Verified Reactivity
Human
Application

ELISA
 
As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Application References

(PubMed link indicates BioLegend citation)
  1. Ishola Jr DA, et al. 2006. Kidney International 2006; doi:10.1038/sj.ki.5001629
  2. Kim I, et al. 2006. Blood doi:10.1182/blood-2006-04-014399
  3. Wimmer A, et al. 2006. Blood 108:3722.
  4. Kim SR, et al. 2009. Biochem Biophys Res Commun. 387:342. PubMed
  5. Samuvel DJ, et al. 2010. J. Biol Chem. 285:3916. PubMed
  6. Tanavalee A, et al. 2011. J. Bone Joint Surg Br. 93:1065. PubMed
  7. Parti PP, et al. 2011. Microbes Infect. 13:545. PubMed
  8. 8. Abe K, et al. 2013. PLoS One. 8:e53620. PubMed
  9. Cho HY, et al. 2014. Cell Immunol. 289:27. PubMed
Product Citations
  1. Abdisalaam S, et al. 2022. Nucleic Acids Res. 50:2681. PubMed
  2. Saxena V, et al. 2022. Cell Rep. 39:110727. PubMed
  3. Chu HS, et al. 2022. Invest Ophthalmol Vis Sci. 63:1. PubMed
  4. Antoon R, et al. 2022. Cytotherapy. 24:699. PubMed
  5. Chang YW, et al. 2018. Am J Physiol Lung Cell Mol Physiol. 314:L654. PubMed
  6. Gradzka-Boberda S, et al. 2022. J Virol. 96:e0121222. PubMed
  7. Walker AR, et al. 2022. Cell Rep. 40:111318. PubMed
  8. Jhuang KF, et al. 2023. Immunology. 169:344. PubMed
  9. Pott F, et al. 2021. Emerg Microbes Infect. 10:2151. PubMed
  10. Zhou J, et al. 2021. Front Immunol. 12:656242. PubMed
  11. Santa Cruz A, et al. 2021. Front Immunol. 613422:12. PubMed
  12. Jasso–Miranda C, et al. 2019. Infect Drug Resist. 12:1833. PubMed
  13. Gill PS, et al. 2022. Front Immunol. 13:872652. PubMed
  14. Bang C, et al. 2017. PLoS One. 12(6):e0178541. PubMed
  15. Zhang W, et al. 2022. Front Pharmacol. 13:1031438. PubMed
  16. Hamel Y, et al. 2021. Cell Rep Med. 2:100370. PubMed
  17. Guo Y, et al. 2020. J Clin Invest. 130:6301. PubMed
  18. Parti R, et al. 2011. Microbes Infect. 13:545. PubMed
  19. Li H, et al. 2021. Mol Med Rep. 1:23. PubMed
  20. Kim S, et al. 2009. Biochem Biophys Res Commun. 387:342. PubMed
  21. Choi J, et al. 2018. Nucleic Acids Res. 46:1912. PubMed
  22. Abdisalaam S, et al. 2020. J Biol Chem. 295:11144. PubMed
  23. Ling C, et al. 2019. Protein Cell. 10:249. PubMed
  24. Tanavalee A, et al. 2011. J Bone Joint Surg Br. 93:1065. PubMed
  25. Soto-Mercado V, et al. 2021. Biomolecules. 11:. PubMed
  26. Martincuks A, et al. 2021. Front Oncol. 11:724104. PubMed
  27. Li Y, et al. 2021. J Neurochem. 159:867. PubMed
  28. Kolar SL, et al. 2019. JCI Insight. 4:5. PubMed
  29. Kamdee K, et al. 2021. Biomed Rep. 15:78. PubMed
  30. Mendes-Frias A, et al. 2022. Sci Rep. 12:17047. PubMed
  31. Feng Y, et al. 2022. Life (Basel). 12:. PubMed
  32. Zhang L, et al. 2022. Nat Commun. 13:360. PubMed
  33. Chen Y, et al. 2021. Front Immunol. 12:623610. PubMed
  34. Hirschberger S, et al. 2021. EMBO Mol Med. 13:e14323. PubMed
  35. Galganska H, et al. 2021. Cell Mol Life Sci. 78:8229. PubMed
  36. Pan Y, et al. 2021. Br J Pharmacol. 178:4907. PubMed
  37. Millrine D, et al. 2016. Proc Natl Acad Sci U S A. 113: 10625 - 10630. PubMed
  38. Mendes B, et al. 2015. Int J Pharmaceutics. 490: 94-101. PubMed
  39. Cho H, Lee S 2014. Cell Immunol. 289:27. PubMed
  40. Abe K, et al. 2013. PLoS One. 8:e53620. PubMed
  41. Samuvel D, et al. 2010. J Biol Chem. 285:3916. PubMed
  42. Thomas A, et al. 2017. PLoS One.. 10.1371/journal.pone.0183542. PubMed
  43. Plesa M, et al. 2021. Front Immunol. 12:715848. PubMed
  44. Bader A, et al. 2015. PLoS One. 10: 0138477. PubMed
  45. Dasari C, et al. 2017. Tumour Biol. 39:1010428317698380. PubMed
  46. Kumar P, et al. 2021. Front Immunol. 12:694865. PubMed
  47. Wang M, et al. 2021. iScience. 24(7):102766. PubMed
  48. Zambrowicz A, et al. 2017. Food Chem. 10.1016/j.foodchem.2017.03.101. PubMed
  49. Kunyanee C, et al. 2016. PLoS One. 11: 0160741. PubMed
  50. CarriÓn M, et al. 2016. J Leukoc Biol. 100(6):1385-1393. PubMed
  51. Takeshita Y, et al. 2016. Neurol Neuroimmunol Neuroinflamm. 4(1):e311. PubMed
  52. Li G, et al. 2021. Gut Microbes. 13:1968257. PubMed
  53. Sadewasser A, et al. 2019. Mol Ther Nucleic Acids. 16:686. PubMed
Sensitivity
4 pg/mL
Standard Range
7.8-500 pg/mL
Materials Not Included
  • Microwell plates: 96-well Nunc MaxiSorp™ is recommended.
  • A microplate reader capable of measuring absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
  • Deionized (DI) water
  • PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 µm filtered.
  • Wash Buffer (Phosphate-Buffered Saline (PBS) + 0.05% Tween-20, pH=7.4). BioLegend Cat. No. 421601 is recommended.
  • Wash bottle or automated microplate washer
  • Stop Solution (e.g. 2N H2SO4) Biolegend Cat. No. 423001 is recommended
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Sealer
  • Absorbent paper

Antigen Details

Cell Sources
T cells, B cells, macrophages, bone marrow stromal cells, fibroblasts, keratinocytes, mesangium, astrocytes, endothelial cells
Biology Area
Immunology, Innate Immunity
Molecular Family
Cytokines/Chemokines
Gene ID
3569 View all products for this Gene ID
UniProt
View information about IL-6 on UniProt.org

Related FAQs

Do the ELISA MAX™ Deluxe Sets come with plates?

No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.

Go To Top Version: 6    Revision Date: 05/09/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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