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ELISA MAX™ Deluxe Set Human IL-8

Pricing & Availability
Regulatory Status
RUO
Other Names
IL-8 ELISA Kit
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Product Citations
publications
human il-8_122909
  • human il-8_122909
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431504 5 plates $305
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Description

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ELISA Development Systems Engineered for Sensitive, Precise and Reliable Serum and Plasma Cytokine Quantitation

BioLegend's ELISA Max™ Sets provide all the time-savings, convenience and performance of a high-end, commercial ELISA kit for a fraction of the price! BioLegend's ELISA Max™ Sets, in two appealing formats, address the need for sensitivity, accuracy, dependability, and versatility of an ELISA development system, while providing maximum cost effectiveness, value, and ease of use.

Both the ELISA Max™ Set Standard and ELISA Max™ Set Deluxe are specifically engineered for accurate recovery and measurement of analytes in complex fluids, such as serum and plasma (as well as activated cell cultures) without any interference by confounding serum factors, such as heterophilic antibody or rheumatoid factor.

Product Details
Technical data sheet

Kit Contents

Kit Contents
  • Human IL-8 ELISA MAX™ Capture Antibody (200X)
  • Human IL-8 ELISA MAX™ Detection Antibody (200X)
  • Human IL-8 Standard
  • Avidin-HRP (1000X)
  • Substrate Solution C
  • Matrix Diluent A (for serum and plasma samples)
  • Coating Buffer A (5X)
  • Assay Diluent A (5X)

Product Details

Verified Reactivity
Human
Application

ELISA
 
As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Application References

(PubMed link indicates BioLegend citation)
  1. Dorosko M, et al. 2010. J. Virol. 84:10533. PubMed
  2. Kim SR, et al. 2010. Biochem Biophys Res Commun. 397:413. PubMed
  3. Hinzey A, et al. 2011. Endocrinolog. 152:483. PubMed
  4. Schneider S, et al. 2011. Infect Immun. 79:2362. PubMed.
  5. Kim SR, Let al. 2012. Biochem Biophys Res Commun. 418:662. PubMed
  6. Saluja B, et al. 2013. Pul Pharmacol Ther. 26:296. PubMed
  7. Meliton AY, et al. 2013. AM J Physiol Lung Cell Mol Physiol. 304:689. PubMed
  8. Montefusco S, et al. 2013. PLoS One. 13:79455. PubMed
Product Citations
  1. Lertjuthaporn S, et al. 2022. Pathogens. 11: . PubMed
  2. Meliton A, et al. 2013. Am J Physiol Lung Cell Mol Physiol. 304:689. PubMed
  3. Bang C, et al. 2017. PLoS One. 12(6):e0178541. PubMed
  4. Sungil Jang, Hanfu Su 2017. MBio. 10.1128/mBio.01779-16. PubMed
  5. Kim S, et al. 2012. Biochem Biophys Res Commun. 418:662. PubMed
  6. Montefusco S, et al. 2013. PLoS One. 13:79455. PubMed
  7. Macleod T, et al. 2020. Cell Rep. 33:108515. PubMed
  8. Chandra V, et al. 2016. Sci Rep. 6: 25765. PubMed
  9. Lau W, et al. 2015. PLoS One. 10: 0141947. PubMed
  10. Connor S 2010. J Virol. 84:10533. PubMed
  11. Schneider S, et al. 2011. Infect Immun. 79:2362. PubMed
  12. Saluja B, et al. 2013. Pulm Pharmacol Ther. 26:296. PubMed
  13. Wielgos ME, et al. 2018. Mol Cancer Ther. 17(5). PubMed
  14. Kim S, et al. 2010. Biochem Biophys Res Commun. 397:413. PubMed
  15. Chen Y, et al. 2021. Front Immunol. 12:623610. PubMed
  16. Laanesoo A, et al. 2021. Clin Transl Med. 11:e427. PubMed
  17. Hirschberger S, et al. 2021. EMBO Mol Med. 13:e14323. PubMed
  18. Luo J, et al. 2019. Mediators Inflamm. 2019:9050965. PubMed
  19. Thomas A, et al. 2017. PLoS One.. 10.1371/journal.pone.0183542. PubMed
  20. Dasari C, et al. 2017. Tumour Biol. 39:1010428317698380. PubMed
  21. Hinzey A, et al. 2011. Endocrinology. 152:483. PubMed
  22. Kunyanee C, et al. 2016. PLoS One. 11: 0160741. PubMed
  23. Huang YC, et al. 2021. Toxins (Basel). 13:. PubMed
  24. Marín-Palma D, et al. 2021. Molecules. 26:. PubMed
  25. Li G, et al. 2021. Gut Microbes. 13:1968257. PubMed
  26. Luvanda MK, et al. 2021. J Fungi (Basel). 7:. PubMed
Sensitivity
8 pg/mL
Standard Range
15.6-1,000 pg/mL
Materials Not Included
  • Microwell plates: 96-well Nunc MaxiSorp™ is recommended.
  • A microplate reader capable of measuring absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
  • Deionized (DI) water
  • PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 µm filtered.
  • Wash Buffer (Phosphate-Buffered Saline (PBS) + 0.05% Tween-20, pH=7.4). BioLegend Cat. No. 421601 is recommended.
  • Wash bottle or automated microplate washer
  • Stop Solution (e.g. 2N H2SO4) Biolegend Cat. No. 423001 is recommended
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Sealer
  • Absorbent paper

Antigen Details

Cell Sources
Monocytes, lymphocytes, granulocytes, fibroblasts, endothelial cells, bronchial epithelial cells, keratinocytes, melanocytes, hepatocytes, mesangial cells, chondrocytes
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Gene ID
3576 View all products for this Gene ID
UniProt
View information about IL-8 on UniProt.org

Related FAQs

Do the ELISA MAX™ Deluxe Sets come with plates?

No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.

Go To Top Version: 4    Revision Date: 07/20/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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