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ELISA MAX™ Deluxe Set Human TNF-α

Pricing & Availability
Regulatory Status
RUO
Other Names
TNF-a ELISA Kit
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Product Citations
publications
human tnf-a_121609
  • human tnf-a_121609
Cat # Size Price Quantity Check Availability Save
430204 5 plates $305
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430215 10 Plates $586
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430216 20 Plates $938
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Description

ELISA Development Systems Engineered for Sensitive, Precise and Reliable Serum and Plasma Cytokine Quantitation

BioLegend's ELISA MAX™ Sets provide all the time-savings, convenience and performance of a high-end, commercial ELISA kit for a fraction of the price!

BioLegend's ELISA MAX™ Sets, in two appealing formats, address the need for sensitivity, accuracy, dependability, and versatility of an ELISA development system, while providing maximum cost effectiveness, value, and ease of use.

Both the ELISA MAX™ Set Standard and ELISA MAX™ Set Deluxe are specifically engineered for accurate recovery and measurement of analytes in complex fluids, such as serum and plasma (as well as activated cell cultures) without any interference by confounding serum factors, such as heterophilic antibody or rheumatoid factor.

Product Details
Technical data sheet

Kit Contents

Kit Contents
  • Human TNF-α ELISA MAX™ Capture Antibody (200X)
  • Human TNF-α ELISA MAX™ Detection Antibody (200X)
  • Human TNF-α Standard
  • Avidin-HRP (1000X)
  • Substrate Solution A
  • Substrate Solution B
  • Coating Buffer A (5X)
  • Assay Diluent A (5X)

Product Details

Verified Reactivity
Human
Application

ELISA
 
As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Application Notes

The assay kits will no longer include ELISA microwell plates. Recommended microwell plates can be purchased separately under Cat. No. 423501. Please contact your BioLegend sales representative for bulk pricing quotations.

Application References

(PubMed link indicates BioLegend citation)
  1. Subramanya S, et al. 2009. J. Virol. PubMed
  2. Schyth BD, et al. 2012. Nucleic Acids Res. 40:463. PubMed.
Product Citations
  1. Lertjuthaporn S, et al. 2022. Pathogens. 11: . PubMed
  2. Xiao Q, et al. 2022. Sci Immunol. 7:eabl3995. PubMed
  3. Gill PS, et al. 2022. Front Immunol. 13:872652. PubMed
  4. Sánchez NC, et al. 2020. DNA Cell Biol. 39:484. PubMed
  5. Singhal J, et al. 2022. Front Cell Infect Microbiol. 12:878136. PubMed
  6. Wang H, et al. 2021. J Immunother Cancer. 9:. PubMed
  7. Guo Y, et al. 2020. J Clin Invest. 130:6301. PubMed
  8. Singh N, et al. 2018. PLoS One. 13:e0199817. PubMed
  9. Naji A, et al. 2016. Sci Rep. 6: 26162. PubMed
  10. Subramanya S, et al. 2010. J Virol. 84:2490. PubMed
  11. Li H, et al. 2021. Mol Med Rep. 1:23. PubMed
  12. Asakura M, et al. 2016. Sci Rep. 6:35633. PubMed
  13. Choi J, et al. 2018. Nucleic Acids Res. 46:1912. PubMed
  14. Pap N, et al. 2021. Food Chem Toxicol. 153:112284. PubMed
  15. Singh T, et al. 2018. J Immunol. 200:2727. PubMed
  16. Kamdee K, et al. 2021. Biomed Rep. 15:78. PubMed
  17. McIntyre B, et al. 2015. Innate Immunity. 21: 504 - 511. PubMed
  18. Hirschberger S, et al. 2022. Front Med (Lausanne). 9:923502. PubMed
  19. XS R, et al. 2015. Diabetes. 64 90. PubMed
  20. Fang W, et al. 2021. Mol Med Rep. 23:. PubMed
  21. Xie X, et al. 2021. Front Immunol. 12:625808. PubMed
  22. Hirschberger S, et al. 2021. EMBO Mol Med. 13:e14323. PubMed
  23. Chabanon RM, et al. 2019. J Clin Invest. 129:1211. PubMed
  24. Lewis A, et al. 2019. Front Immunol. 10:2321. PubMed
  25. Dold MN, et al. 2022. Apoptosis. 27:112. PubMed
  26. Millrine D, et al. 2016. Proc Natl Acad Sci U S A. 113: 10625 - 10630. PubMed
  27. Schyth B, et al. 2012. Nucleic Acids Res. 40:4653. PubMed
  28. Wurster S, et al. 2017. Virulence. 8:1708. PubMed
  29. Dasari C, et al. 2017. Tumour Biol. 39:1010428317698380. PubMed
  30. Wang M, et al. 2021. iScience. 24(7):102766. PubMed
  31. Girsang E, et al. 2021. PeerJ. 9:e11419. PubMed
  32. Lei W, et al. 2022. Blood Cancer J. 12:35. PubMed
  33. Kunyanee C, et al. 2016. PLoS One. 11: 0160741. PubMed
  34. CarriÓn M, et al. 2016. J Leukoc Biol. 100(6):1385-1393. PubMed
  35. Li G, et al. 2021. Gut Microbes. 13:1968257. PubMed
Sensitivity
2 pg/ml
Standard Range
7.8-500 pg/mL
Materials Not Included
  • Microwell plates: 96-well Nunc MaxiSorp™ is recommended.
  • A microplate reader capable of measuring absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
  • Deionized (DI) water
  • PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 µm filtered.
  • Wash Buffer (Phosphate-Buffered Saline (PBS) + 0.05% Tween-20, pH=7.4). BioLegend Cat. No. 421601 is recommended.
  • Wash bottle or automated microplate washer
  • Stop Solution (e.g. 2N H2SO4) Biolegend Cat. No. 423001 is recommended
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Sealer
  • Absorbent paper

Antigen Details

Cell Sources
Activated monocytes, neutrophils, macrophages, T cells, B cells, NK cells, LAK cells
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Gene ID
7124 View all products for this Gene ID
UniProt
View information about TNF-alpha on UniProt.org

Related FAQs

Do the ELISA MAX™ Deluxe Sets come with plates?

No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.

Go To Top Version: 5    Revision Date: 04/04/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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