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MojoSort™ anti-Biotin Nanobeads

Pricing & Availability
Regulatory Status
RUO
Other Names
Anti-Vitamin B7 Nanobeads, Anti-Vitamin H nanobeads, Anti-Biotinylated cell nanobeads
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Product Citations
publications
A_MojoSort_Biotin_Nanobeads_1_071724
A single cell suspension from human peripheral blood mononuclear cells (PBMCs) was prepared to isolate CD14+ cells using MojoSort™ anti-Biotin Nanobeads and biotinylated anti-human CD14 (clone 63D3) (Cat. No. 367105). Cells were stained with anti-human CD14 (clone HCD14) PE (Cat. No. 325605) and anti-human CD3 (clone UCHT1) FITC (Cat. No. 300452). The analysis was pre-gated with anti-human CD45 (clone HI30) APC/Cyanine7 (Cat. No. 304014) staining. Dead cells were excluded using Helix NP™ Blue (Cat. No. 425305).
  • A_MojoSort_Biotin_Nanobeads_1_071724
    A single cell suspension from human peripheral blood mononuclear cells (PBMCs) was prepared to isolate CD14+ cells using MojoSort™ anti-Biotin Nanobeads and biotinylated anti-human CD14 (clone 63D3) (Cat. No. 367105). Cells were stained with anti-human CD14 (clone HCD14) PE (Cat. No. 325605) and anti-human CD3 (clone UCHT1) FITC (Cat. No. 300452). The analysis was pre-gated with anti-human CD45 (clone HI30) APC/Cyanine7 (Cat. No. 304014) staining. Dead cells were excluded using Helix NP™ Blue (Cat. No. 425305).
  • B_MojoSort_Biotin_Nanobeads_2_071724
    Starting with human whole blood, CD8+ cells were isolated using MojoSort™ anti-Biotin Nanobeads and biotinylated anti-human CD8 (clone SK1) (Cat. No. 344720). Cells were stained with anti-human CD8 (clone RPA-T8) PE (Cat. No. 301004) and anti-human CD3 (clone UCHT1) APC (Cat. No. 300412). The analysis was pre-gated with anti-human CD45 (clone HI30) APC/Cyanine7 (Cat. No. 304014) staining. Dead cells were excluded using Helix NP™ Blue (Cat. No. 425305).
  • C_MojoSort_Biotin_Nanobeads_3_071724
    A single cell suspension from human peripheral blood mononuclear cells (PBMCs) was positively selected for CD19+ cells using the MojoSort™ anti-Biotin Nanobeads and biotinylated anti-human CD19 (Cat. No. 302204). Cells were stained for flow cytometry with anti-human CD19 (clone HIB19) APC (Cat. No. 302212) and anti-human CD3 (clone UCHT1) PE (Cat. No. 300408). Dead cells were excluded using Helix NP™ Blue (Cat. No. 425305).
Cat # Size Price Quantity Check Availability Save
480032 100 µL $160
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480034 1 mL $464
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Description

MojoSort™ anti-Biotin Nanobeads can be used for positive or negative selection of targeted cells with biotin-conjugated antibodies. For positive selection, the magnetically labeled fraction is retained by the use of a magnetic separator. For negative selection, the untouched cells are collected by decanting the liquid in a clean tube. Either positive or negative selection cells can be used for downstream applications such as functional assays, gene expression, phenotypic characterization, etc.

A complete list of BioLegend’s biotin-conjugated antibodies can be found here.

MojoSort™ reagents are also compatible with column-based cell separation systems available from other vendors. Optimized protocols for cell separation using columns from in-house testing are provided for each kit under the “Related Protocols” section, as well as representative data on the product webpage (where available). Data generated using column separators are indicated on the figure legend.

Due to the property of the beads, MojoSort™ reagents typically require dilution for optimal use on column separators. Where available, recommended dilution factors for each kit component based on in-house testing are provided under the “Application Notes” section of the webpage.

Product Details
Technical data sheet

Kit Contents

Kit Contents

For Cat. No. 480032:

  • 1 vial containing 100 µL of anti-Biotin Nanobeads


For Cat. No. 480034:

  • 1 vial containing 1 mL of anti-Biotin Nanobeads

Product Details

Verified Reactivity
Species independent, all species
Formulation
Particle: Aqueous solution containing BSA and 0.05% sodium azide.
Preparation
Anti-Biotin conjugated particles: protein-coated magnetic nanobeads.
Storage & Handling
Anti-Biotin Nanobeads should be stored undiluted between 2°C and 8°C
Application

Cell Separation (MojoSort™) - Quality tested
Recommended Usage

The concentration of anti-Biotin Nanobeads to be used should be optimized by end users.

Application Notes

The MojoSort™ anti-Biotin Nanobeads are designed for the positive or negative isolation of cells when used with biotin-conjugated antibodies or similar reagents.

Nanobead dilution to use in column: To be optimized by the end user

A complete list of BioLegend’s biotin-conjugated antibodies can be found here.

Related FAQs

Is there a way to detach your magnetic particles from the cell surface?

MojoSort™ Nanobeads: These magnetic particles cannot be removed from cells. This includes standalone Nanobeads and MojoSort kits using Nanobeads. We have found that cells are functional without the need to detach the magnetic Nanobeads.

 

MojoSort Microbeads: These magnetic particles may be released following the detailed product-specific protocol.

What is the size of your magnetic particles?

MojoSort Nanobeads: the average diameter is approximately 130 nm.

 

MojoSort Microbeads: the average diameter is approximately 1 μm.

Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?

MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems. Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems.  Please contact BioLegend Technical Service for more product-specific information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.

What antibodies are present in the depletion cocktails provided for isolation kits?

Please contact our technical service team for further assistance.

Go To Top Version: 1    Revision Date: 05/31/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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