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ELISA MAX™ Deluxe Set Mouse IFN-γ

Pricing & Availability
Regulatory Status
RUO
Other Names
IFN-g ELISA Kit
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Product Citations
publications
mouse ifn-g_122109
  • mouse ifn-g_122109
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430804 5 plates $305
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430815 10 Plates $586
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430816 20 Plates $938
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Description

ELISA Development Systems Engineered for Sensitive, Precise and Reliable Serum and Plasma Cytokine Quantitation

BioLegend's ELISA MAX™ Sets provide all the time-savings, convenience and performance of a high-end, commercial ELISA kit for a fraction of the price!

BioLegend's ELISA MAX™ Sets, in two appealing formats, address the need for sensitivity, accuracy, dependability, and versatility of an ELISA development system, while providing maximum cost effectiveness, value, and ease of use.

Both the ELISA MAX™ Set Standard and ELISA MAX™ Set Deluxe are specifically engineered for accurate recovery and measurement of analytes in complex fluids, such as serum and plasma (as well as activated cell cultures) without any interference by confounding serum factors, such as heterophilic antibody or rheumatoid factor.

Product Details
Technical data sheet

Kit Contents

Kit Contents
  • Mouse IFN-γ ELISA MAX™ Capture Antibody (200X)
  • Mouse IFN-γ ELISA MAX™ Detection Antibody (200X)
  • Mouse IFN-γ Standard
  • Avidin-HRP (1000X)
  • Substrate Solution F
  • Coating Buffer A (5X)
  • Assay Diluent A (5X)

Product Details

Verified Reactivity
Mouse
Application

ELISA
 
As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Application Notes

The assay kits will no longer include ELISA microwell plates. Recommended microwell plates can be purchased separately under Cat. No. 423501. Please contact your BioLegend sales representative for bulk pricing quotations.

Application References

(PubMed link indicates BioLegend citation)
  1. Lau AH, et al. 2006. J. Leukoc. Biol. 79:941.
  2. Inoue Y, et al. 2007. J. Immunol. 179:764.
  3. Perchonock CE, et al. 2007. J. Immunol. 179:1768.
  4. Perruche S, et al. 2009. J. Immunol. 183:953. PubMed
  5. Tallima H, et. al. 2009. Cytokine 48:239. PubMed
  6. Zhang Y, et al. 2010. Infect Immun. 78:2329. PubMed
  7. El Ridi R, et al. 2010. Microbes Infect. epub. PubMed
  8. McPhee JB, et al. 2010. Infect Immun. PubMed
  9. Yu M, et al. 2011. Vaccine. 29:2429. PubMed
  10. Puangpetch A, et al. 2012. Clin Vaccine Immunol. 19:675. PubMed.
Product Citations
  1. Klement JD, et al. 2018. J Clin Invest. 128:5549. PubMed
  2. Madrid-Paulino E, et al. 2022. J Leukoc Biol. 112:475. PubMed
  3. Luo WJ, et al. 2022. Elife. 11: . PubMed
  4. Cheng P, et al. 2023. Pharmacol Res. 188:106643. PubMed
  5. Bhardwaj M, et al. 2023. J Clin Invest. :. PubMed
  6. Sillibourne JE, et al. 2022. Biotechniques. 72:143. PubMed
  7. Mooney JP, et al. 2022. Wellcome Open Res. 7:134. PubMed
  8. Nakka K, et al. 2022. Science. 377:666. PubMed
  9. Tubiana J, et al. 2022. Cell Rep. 41:111512. PubMed
  10. Do-Thi VA, et al. 2023. Cancer Res Commun. 3:80. PubMed
  11. Zhou T, et al. 2020. Nature. 583:609. PubMed
  12. Puangpetch A, et al. 2012. Clin Vaccine Immunol. 1.260416667. PubMed
  13. Perruche S, et al. 2009. J Immunol. 183:953. PubMed
  14. Chen M, et al. 2022. J Nanobiotechnology. 20:273. PubMed
  15. Xu B, et al. 2021. Front Immunol. 11:583510. PubMed
  16. Radwanska M, et al. 2021. Vaccines (Basel). 9:. PubMed
  17. Yu M, Vajdy M 2011. Vaccine. 29:2429. PubMed
  18. Du Q, et al. 2019. Front Microbiol. 10:2050. PubMed
  19. Puca E, et al. 2020. J Gastrointest Oncol. 1.015972222. PubMed
  20. Kohn M, et al. 2021. Front Immunol. 580594:12. PubMed
  21. Clancy–Thompson E, et al. 2018. Cancer Immunol Res. 1.308333333. PubMed
  22. Sun Z, et al. 2020. Front Immunol. 1.568055556. PubMed
  23. McPhee J, et al. 2010. Infect Immun. 78:3529. PubMed
  24. Daher C, et al. 2019. Cancer Immunol Res. 1.575694444. PubMed
  25. Park GT, et al. 2021. Mar Drugs. 19:. PubMed
  26. Leroux LP, et al. 2020. Front Cell Infect Microbiol. 0.755555556. PubMed
  27. Wei W, et al. 2022. mSystems. 7:e0046922. PubMed
  28. Li Y, et al. 2021. Sci Transl Med. 13:. PubMed
  29. Park GT, et al. 2019. Front Immunol. 10:2095. PubMed
  30. Wang J, et al. 2022. J Clin Invest. :. PubMed
  31. Ci T, et al. 2020. Sci Adv. 6:00. PubMed
  32. Shiozawa S, et al. 2022. iScience. 25:103537. PubMed
  33. Zeng Q, et al. 2022. iScience. 25:105151. PubMed
  34. Fernandes RA, et al. 2020. Nature. 779:586. PubMed
  35. Bliska Y 2010. Infect Immun. 78:2359. PubMed
  36. Wang Y, et al. 2021. Nat Commun. 12:4964. PubMed
  37. Abdelfattah N, et al. 2022. Nat Commun. 13:767. PubMed
  38. Garrido V, et al. 2021. Mol Syst Biol. 17:e10145. PubMed
  39. Minns D, et al. 2021. Nat Commun. 12:1285. PubMed
  40. Lanfermann C, et al. 2021. Vaccines (Basel). 9:. PubMed
  41. Gu BH, et al. 2022. Biomedicines. 10:. PubMed
  42. Lanfermann C, et al. 2021. Vaccines (Basel). 9:. PubMed
  43. Shi D, et al. 2022. Nat Commun. 13:5644. PubMed
  44. Kohn M, et al. 2021. Front Immunol. 626627:12. PubMed
  45. Li X, et al. 2022. Oncoimmunology. 11:2118210. PubMed
  46. Waaler J, et al. 2020. Commun Biol. 3:196. PubMed
  47. Reed MD, et al. 2020. Nature. 577:249. PubMed
  48. Chaudhari R, et al. 2021. Nanomaterials (Basel). 11:. PubMed
Sensitivity
4 pg/mL
Standard Range
15.6-1,000 pg/mL
Materials Not Included
  • Microwell plates: 96-well Nunc MaxiSorp™ is recommended.
  • A microplate reader capable of measuring absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
  • Deionized (DI) water
  • PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 µm filtered.
  • Wash Buffer (Phosphate-Buffered Saline (PBS) + 0.05% Tween-20, pH=7.4). BioLegend Cat. No. 421601 is recommended.
  • Wash bottle or automated microplate washer
  • Stop Solution (e.g. 2N H2SO4) Biolegend Cat. No. 423001 is recommended
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Sealer
  • Absorbent paper

Antigen Details

Cell Sources
CD8+ and CD4+ T cells, NK cells
Cell Type
Tregs
Biology Area
Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Gene ID
15978 View all products for this Gene ID
UniProt
View information about IFN-gamma on UniProt.org

Related FAQs

Do the ELISA MAX™ Deluxe Sets come with plates?

No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.

Go To Top Version: 4    Revision Date: 11/17/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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