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PE anti-human CD151 (PETA-3) Antibody

Pricing & Availability
Clone
50-6 (See other available formats)
Regulatory Status
RUO
Other Names
Platelet-endothelial tetraspan antigen 3, GP27, Membrane glycoprotein SFA-1, Tetraspanin-24, Tspan-24
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
50-6_PE_CD151_Antibody_FC_080113.jpg
Human peripheral blood platelets stained with anti-human CD151 (clone 50-6) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
  • 50-6_PE_CD151_Antibody_FC_080113.jpg
    Human peripheral blood platelets stained with anti-human CD151 (clone 50-6) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
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350407 25 tests $146
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350408 100 tests $329
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Description

CD151, also known as PETA-3, is a 29 kD protein member of the tetraspanin family (TM4SF). As other members in the family, PETA-3 is composed of two extracellular loops (one larger than the other), four transmembrane domains, and two cytoplasmic tails. Also, as other members in the family, CD151 is involved in cell adhesion, migration, and tumor cell metastasis. PETA-3 is expressed by many different cell types such as immature hematopoietic cells, megakaryocytes, platelets, keratinocytes, epithelial cells, muscle cells, Schwann cells, and vascular endothelium. On the cell surface, PETA-3 associates with CD9, CD181, and the integrins α3β1, α5β1, and α6β4.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human epidermoid carcinoma cell line HEp-3
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: in vivo inhibition of metastasis of HEp-3 cells1, Western blotting1, immunocytochemical staining of HEp-3 cells1, and CD151-transfected COS cells fixed with 0.25% glutaraldehyde.

Application References

(PubMed link indicates BioLegend citation)
  1. Testa JE, et al. 1999. Cancer Res. 59:3812. (Block, ICC, WB)
RRID
AB_2562331 (BioLegend Cat. No. 350407)
AB_2562331 (BioLegend Cat. No. 350408)

Antigen Details

Structure
Member of the tetraspanin family (TM4SF), 29 kD protein, consist of two extracellular loop, four transmembrane domains and two cytoplasmic tails
Distribution

Immature hematopoietic cells, megakaryocytes, platelets, keratinocytes, epithelial cells, muscle cells, Schwann cells, vascular endothelium

Function
Cell adhesion, migration
Interaction
CD9, CD181, integrins α3β1, α5β1 and α6β4
Cell Type
Epithelial cells, Hematopoietic stem and progenitors, Megakaryocytes, Mesenchymal Stem Cells, Platelets
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Zöller M. 2009. Nat. Rev. Cancer 9:40.
2. Yañez-Mó M, et al. 2008. Blood 112:3217.
3. Nishiuchi R, et al. 2005. Proc. Natl. Acad. Sci. USA 102:1939.

Gene ID
977 View all products for this Gene ID
UniProt
View information about CD151 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 08/05/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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