Alexa Fluor 647 anti-human/mouse SSEA-3 Antibody anti-SSEA-3

Pricing & Availability

Clone: MC-631 (See other available formats)
Regulatory Status: RUO
Other Names: Stage-Specific Embryonic Antigen-3
Isotype: Rat IgM, κ
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NCCIT (human teratocarcinoma cell line) stained with MC-631 Alexa Fluor® 647

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330307

25 tests

$165

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330308

100 tests

$370

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Description

Stage-Specific Embryonic Antigen-3 (SSEA-3) is an internal core structure of glob-series glycolipids (R-Galβ1-3GalNAcβ1-3Galα1-4Galβ1-R). SSEA-3 is expressed on human teratocarcinoma cells (embryonal carcinoma, EC), embryonic stem cells (ESC), embryonic germ cells (EG), and preimplantation embryos. The expression of SSEA-3 on human ESC is downregulated upon differentiation. SSEA-3 is also expressed by mouse on unfertilized eggs and preimplantation to early cleaved embryos.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human, Mouse
Reported Reactivity: African Green
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: 4-8 cell stage mouse embryos
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunofluorescence microscopy¹.
RRID: AB_1227772 (BioLegend Cat. No. 330307)
AB_1227772 (BioLegend Cat. No. 330308)

Antigen Details

Distribution: Embryonic stemcells
Cell Type: Embryonic Stem Cells
Biology Area: Cell Biology, Immunology, Stem Cells
Gene ID: 93961 View all products for this Gene ID
UniProt: View information about SSEA-3 on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Description	Clone	Applications

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Other Formats

View All SSEA-3 Reagents Request Custom Conjugation

Description	Clone	Applications
PE anti-human/mouse SSEA-3	MC-631	FC
Alexa Fluor® 488 anti-human/mouse SSEA-3	MC-631	FC
Purified anti-human/mouse SSEA-3	MC-631	FC,ICC,ELISA
Alexa Fluor® 647 anti-human/mouse SSEA-3	MC-631	FC
APC/Cyanine7 anti-human/mouse SSEA-3	MC-631	FC
Biotin anti-human/mouse SSEA-3	MC-631	FC
FITC anti-human/mouse SSEA-3	MC-631	FC
PerCP/Cyanine5.5 anti-human/mouse SSEA-3	MC-631	FC
PE/Cyanine7 anti-human/mouse SSEA-3	MC-631	FC

Certificate of Analysis

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Go To Top Version: 3 Revision Date: 06/27/2014

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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