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- Clone
- W20199I (See other available formats)
- Regulatory Status
- RUO
- Other Names
- CD40L, TNFSF5, T-BAM, gp39
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Human peripheral blood mononuclear cells were treated with Cell Activation Cocktail (without Brefeldin A) (Cat. No. 423302). Cells were surface stained with anti-human CD154 (clone W20199I) PE (left) or rat IgG2b, κ PE isotype control (right). Cells were costained with anti-human CD4 APC.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 388203 | 25 tests | $125 | ||||
| 388204 | 100 tests | $255 | ||||
CD154, also known as CD40L, TNFSF5, T-BAM, and gp39, is a 32-39 kD type II transmembrane glycoprotein member of the TNF superfamily expressed on activated T cells. CD154 interacts as a costimulatory molecule with CD40 on B Cells to induce B cell activation, resulting in proliferation, immunoglobulin production, and Ig Isotype switching. CD40L also may regulate T cell cytokine production.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant human CD40L protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
Clone W20199I is able to block the binding of clone 24-31 on target cells.
Clone W20199I is able to block the binding of CD40 and CD40L interaction on target cells. - RRID
-
AB_3105982 (BioLegend Cat. No. 388203)
AB_3105982 (BioLegend Cat. No. 388204)
Antigen Details
- Structure
- 32-39 kD type II transmembrane glycoprotein of the TNF superfamily
- Distribution
-
Activated T cells
- Function
- B cell costimulation
- Ligand/Receptor
- CD40
- Cell Type
- T cells, Tregs
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
- van Kooten C and Banchereau J. 2000. J Leukoc Biol. 67:2-17.
- Ford GS, et al. 1999. J Immunol. 162:4037-44.
- Gene ID
- 959 View all products for this Gene ID
- UniProt
- View information about CD154 on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
Other Formats
View All CD154 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| PE anti-human CD154 | W20199I | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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PE anti-human CD154
Human peripheral blood mononuclear cells were treated with C...
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