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PE anti-human CD307c/FcRL3 Antibody

Pricing & Availability
Clone
H5/FcRL3 (See other available formats)
Regulatory Status
RUO
Other Names
Fc receptor-like 3 (FCRL3), Immune receptor translocation-associated protein 3 (IRTA3), SH2 domain-containing phosphatase anchor protein 2 (SPAP2), IFGP family protein 3 (IFGP3)
Isotype
Mouse IgG2b, κ
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Product Citations
publications
H5slashFcRL3_PE_CD307c_Antibody_1_111717
Human peripheral blood lymphocytes were surface stained with CD3 Alexa Fluor® 488 and H5/FcRL3 PE (top) or Mouse IgG2b, κ PE Isotype control (bottom).
  • H5slashFcRL3_PE_CD307c_Antibody_1_111717
    Human peripheral blood lymphocytes were surface stained with CD3 Alexa Fluor® 488 and H5/FcRL3 PE (top) or Mouse IgG2b, κ PE Isotype control (bottom).
  • H5slashFcRL3_PE_CD307c_Antibody_2_111717
  • H5slashFcRL3_PE_CD307c_Antibody_3_111617
    Human peripheral blood lymphocytes were surface stained with CD4 Alexa Fluor® 647 and H5/FcRL3 PE then treated with True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401). Cells were then stained with FOXP3 (clone 206D) Brilliant Violet 421™. Data shown was gated on CD4+ peripheral blood lymphocytes.
  • H5slashFcRL3_PE_CD307c_Antibody_4_111617
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374405 25 tests $146
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374406 100 tests $360
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Description

CD307c is a type 1 transmembrane glycoprotein in the FcRL family of the Ig gene superfamily that is conserved in humans and not found in mice. It is expressed on NK cells, T cell, regulatory T cell, B cell and plasma cell subsets. Intracellular ITAM and ITIM motifs in conjunction with extracellular Ig domains are thought to play a role in the regulation of immune response, and autoimmune function. Impairment of normal FcRL3 function has been linked to systemic lupus erythematosus, rheumatoid arthritis, and autoimmune thyroid disease.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
FcRL3 full length expression plasmid DNA, followed by cell boost using transiently transfected cells with the same plasmid.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Nagata S, et al. 2009. J. Immunol. 182:7518-26.
RRID
AB_2687380 (BioLegend Cat. No. 374405)
AB_2687380 (BioLegend Cat. No. 374406)

Antigen Details

Distribution

NK, T, regulatory T, B, and plasma cell subsets.

Interaction
Tyrosine-protein phosphatase non-receptor type 6 (PTPN6).
Cell Type
B cells, NK cells, Plasma cells, T cells, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, Fc Receptors
Antigen References

1. Bin Dhuban K, et al. 2015. J. Immunol. 194(8):3687-96.
2. Yuan M, et al. 2016. Mol. Neurobiol. 53:2029-35.
3. Fang Y, et al. 2016. Immunobiology. 221(1):56-62.

Gene ID
115352 View all products for this Gene ID
UniProt
View information about CD307c/FcRL3 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 0    Revision Date: 07/07/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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