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PE anti-human CD307d (FcRL4) Antibody

Pricing & Availability
Clone
413D12 (See other available formats)
Regulatory Status
RUO
Other Names
FcRL4 (Fc receptor-like 4), IRTA1, IGFP2
Isotype
Mouse IgG2b, κ
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Product Citations
publications
413D12_PE_022309.jpg
FcRL4 transfected P815 cells stained with 413D12 PE
  • 413D12_PE_022309.jpg
    FcRL4 transfected P815 cells stained with 413D12 PE
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340203 25 tests $146
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340204 100 tests $360
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Description

FcRL4 (Fc receptor-like 4) is a 57 kD single pass type I transmembrane protein that exhibits 4 Ig like C2 domains. Also known as ITRA1, IGFP2, and FcRH4, FcRL4 is predominately found on a subset of memory B cells. Activation of FcRL4 contributes to B cell receptor inhibition through activation of the tyrosine kinases SHP-1 and 2. FcRL4's nascent loci is 1q23.1; however, breakpoint mutations are associated with multiple myeloma and non-Hodgkins lymphoma. Increased FcRL4 expression on memory B cells has also been observed in HIV viremic patients. FcRL4 predominately binds aggregated IgA, binds weakly with IgG3 and IgG4, and does not bind IgG1 and IgG2. 

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
P815 cells transfected with FcRL4
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (with relevant formats) include: immunoprecipitation and blocking. mAb 413D12 is able to block the binding of FcRL4 to aggregated IgA, IgG3, and IgG4.

Application References

(PubMed link indicates BioLegend citation)
  1. Sohn HW, et al. 2011. Blood. 118:6332. PubMed
  2. Wilson TJ, et al. 2012. J. Immunol. 188:4741. (Block)
Product Citations
  1. Oliviero B, et al. 2011. J Hepatol. 55:53. PubMed
  2. SoRelle ED, et al. 2022. Front Immunol. 13:1001145. PubMed
  3. Li H, et al. 2016. J Immunol. 196: 4064 - 4074. PubMed
  4. Parmigiani A, et al. 2013. PLoS One. 13:79816. PubMed
  5. Sohn H, et al. 2011. Blood. 118:6332. PubMed
  6. Magri G et al. 2017. Immunity. 47(1):118-134 . PubMed
  7. Amara K, et al. 2017. J Autoimmun. 10.1016/j.jaut.2017.03.004. PubMed
  8. Csomós K, et al. 2022. Nat Immunol. 23:1256. PubMed
  9. Jenks SA et al. 2018. Immunity. 49(4):725-739 . PubMed
  10. Upasani V, et al. 2019. Front Immunol. 2.152777778. PubMed
RRID
AB_2104578 (BioLegend Cat. No. 340203)
AB_2104578 (BioLegend Cat. No. 340204)

Antigen Details

Structure
57 kD, Fc receptor like, four Ig like domains
Distribution

Subset of memory B cells, some multiple myeloma and non-Hodgkins lymphoma

Interaction
Tyrosine phophorylated FcRL4 interacts with SHP-1, SHP-2 to inhibit the B cell receptor
Bioactivity
B-cell receptor inhibition
Cell Type
B cells
Biology Area
Immunology
Molecular Family
CD Molecules, Fc Receptors
Antigen References

1. Moir S, et al. 2008. J. Exp. Med. 205:1797.
2. Ehrhardt GRA, et al. 2003. P. Natl. Acad. Sci. USA 100:13489.
3. Ehrhardt GRA, et al. 2005. J. Exp. Med. 202:783.
4. Polson AG, et al. 2006. J. Int. Immunol. 18:1363.

Gene ID
83417 View all products for this Gene ID
UniProt
View information about CD307d on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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