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PE anti-mouse CD154 Antibody

Pricing & Availability
Clone
MR1 (See other available formats)
Regulatory Status
RUO
Other Names
CD40 ligand (CD40L), gp39, T-BAM, TRAP, Ly-62, TNFSF5
Isotype
Armenian Hamster IgG
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Product Citations
publications
MR1
PMA- and ionomycin-stimulated (6 hrs) BALB/c T cells stained with MR1 PE
  • MR1
    PMA- and ionomycin-stimulated (6 hrs) BALB/c T cells stained with MR1 PE
Compare all formats See PE spectral data
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106505 50 µg $110
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106506 200 µg $297
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Description

CD154 is a 39 kD TNF superfamily member also known as CD40 ligand, gp39, T-BAM, TRAP, and Ly-62. CD154 is an accessory molecule expressed predominantly on activated CD4+ lymphocytes that bind CD40. CD154 plays an important role in T-B cell costimulation. The MR1 antibody has been reported to inhibit the activation of T and B lymphocytes in vitro and antigen-specific lymphocyte responses in vivo.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Activated mouse Th1 clone D1.6
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining1,2 of acetone-fixed frozen sections, and in vitro and in vivo blocking of ligand binding3-5. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 106506) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 106504) or biotinylated anti-Armenian hamster IgG (Cat. No. 405501) second step, followed by SAv-PE (Cat. No. 405204). The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 106515-106520).

Application References

(PubMed link indicates BioLegend citation)
  1. Lettesjö H, et al. 2000. J. Immunol. 165:4095. (IHC)
  2. Dunn RJ, et al. 1997. J. Histochem. Cytochem. 45:129. (IHC)
  3. Noelle RJ, et al. 1992. P. Natl. Acad. Sci. USA 89:6550. (Block)
  4. Roy M, et al. 1995. Eur. J. Immunol. 25:596. (Block)
  5. Foy TM, et al. 1994. J. Exp. Med. 180:157. (Block)
  6. Lawson BR, et al. 2007. J. Immunol. 178:5366.
Product Citations
  1. Xie A et al. 2017. Endocrinology. 158(10):3140-3151 . PubMed
  2. Li H, et al. 2021. Nat Commun. 12:2773. PubMed
  3. Sasaki K, et al. 2019. Nat Commun. 10:3878. PubMed
  4. Gengenbacher M, et al. 2014. MBio. 5:1262. PubMed
  5. Mintz MA, et al. 2019. Immunity. 51:310. PubMed
  6. Hu Q, et al. 2018. Nat Biomed Eng. 0.660416667. PubMed
  7. Danelli L, et al. 2015. Cancer Immunol Res. 3:85. PubMed
  8. Lu X, et al. 2015. J Immunol. 194:2011. PubMed
  9. Trefzer A, et al. 2021. Cell Reports. 34(6):108748. PubMed
  10. Frost JN, et al. 2021. Med (N Y). 2:164. PubMed
  11. Chen C, et al. 2011. PLoS One. 6:e17712. PubMed
  12. Iwata H, et al. 2013. J Immunol. 191:2978. PubMed
  13. Kimura S, et al. 2020. Am J Transplant. 20:977. PubMed
  14. Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed
  15. Toriyama K, et al. 2020. Commun Biol. 0.398611111. PubMed
RRID
AB_313271 (BioLegend Cat. No. 106505)
AB_313271 (BioLegend Cat. No. 106506)

Antigen Details

Structure
TNF superfamily, 39 kD
Distribution

Activated CD4+ T cells

Function
T-B cell costimulation
Ligand/Receptor
CD40
Cell Type
T cells, Tregs
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Noelle RJ, et al. 1992. P. Natl. Acad. Sci. USA 89:6550.
3. Bancherou J, et al. 1994. Annu. Rev. Immunol. 12:881.
4. Clark EA, et al. 1996. P. Natl. Acad. Sci. USA 83:4494.

Gene ID
21947 View all products for this Gene ID
UniProt
View information about CD154 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 02/11/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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