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PE anti-mouse CD287 (TLR7) Antibody

Pricing & Availability
Clone
A94B10 (See other available formats)
Regulatory Status
RUO
Other Names
TLR7, Toll-like receptor 7, CD287
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A94B10_CD287_PE_Antibody_042920
C57BL/6 splenocytes were stained with CD45R/B220 APC and CD317 Brilliant Violet 421™, then treated with BioLegend's Fixation Buffer (Cat. No. 420801) and Permeabilization Wash Buffer (Cat. No. 421002), and stained with CD287 (TLR7) (clone A94B10) (left) PE or mouse IgG1, κ PE isotype control (right). Data shown was gated in the CD45R/B220+ population.
  • A94B10_CD287_PE_Antibody_042920
    C57BL/6 splenocytes were stained with CD45R/B220 APC and CD317 Brilliant Violet 421™, then treated with BioLegend's Fixation Buffer (Cat. No. 420801) and Permeabilization Wash Buffer (Cat. No. 421002), and stained with CD287 (TLR7) (clone A94B10) (left) PE or mouse IgG1, κ PE isotype control (right). Data shown was gated in the CD45R/B220+ population.
See PE spectral data
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160003 25 µg $146
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160004 100 µg $365
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Description

Toll-like receptor (TLR) 7, also known as CD287 is a type 1 transmembrane protein, member of the TLR receptor family. TLR7 is expressed intracellularly in plasmacytoid dendritic cells, myeloid cells, and B lymphocytes and plays an important role in the innate immune response by binding single-stranded RNA (ssRNA). TLR7 signals through MyD88, which leads to the activation and secretion of IFN-α/β and other inflammatory cytokines. 

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Ba/F3 cells expressing flag-6xHis-conjugated mouse TLR7
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Kanno A, et al. 2013. Int Immunol. 25:413 
RRID
AB_2876562 (BioLegend Cat. No. 160003)
AB_2876562 (BioLegend Cat. No. 160004)

Antigen Details

Structure
Type 1 transmembrane protein, member of the Toll-like receptor (TLR) family
Distribution

Myeloid and plasmacytoid dendritic cells, and B lymphocytes

Function
Pattern recognition receptor that detects single-stranded RNA, induces proinflammatory cytokine production
Ligand/Receptor
Single-stranded RNA
Cell Type
B cells, Dendritic cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Innate Immune Signaling
Antigen References
  1. Majer O, et al. 2019. Nature. 575:371. 
  2. Akilesh HM, et al. 2019. Science 363(6423) 
  3. Hemmi H, et al. 2002. Nat. Immunol. 3:196. 
Gene ID
170743 View all products for this Gene ID
UniProt
View information about CD287 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 04/29/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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