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PE anti-mouse CD289 (TLR9) Antibody

Pricing & Availability
Clone
S18025A (See other available formats)
Regulatory Status
RUO
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
S18025A_PE_CD289_Antibody_1_070820.png
C57BL/6 mouse splenocytes were stained with CD317 Pacific Blue™, fixed, permeabilized and intracellularly stained with CD289 (TLR9) (clone S18025A) PE (left) or rat IgG1, κ isotype control PE (right).
  • S18025A_PE_CD289_Antibody_1_070820.png
    C57BL/6 mouse splenocytes were stained with CD317 Pacific Blue™, fixed, permeabilized and intracellularly stained with CD289 (TLR9) (clone S18025A) PE (left) or rat IgG1, κ isotype control PE (right).
  • S18025A_PE_CD289_Antibody_2_070820.png
    C57BL/6 mouse splenocytes were stained with CD45R/B220 APC/Cyanine7, fixed, permeabilized and intracellularly stained with CD289 (TLR9) (clone S18025A) PE (left) or rat IgG1, κ isotype control PE (right).
Compare all formats See PE spectral data
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159103 25 µg $153
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159104 100 µg $372
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Description

TLR9 is a member of the toll-like receptor family that aids in recognition of pathogen associated molecular patterns (PAMPs). TLR9 recognizes unmethylated CpG sequences in DNA (ie. intracellular bacteria and DNA viruses). TLR9 signals through the MyD88 pathway leading to inflammatory cytokine production, especially production of type I interferons by pDCs.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Proprietary
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID
AB_2876552 (BioLegend Cat. No. 159103)
AB_2876552 (BioLegend Cat. No. 159104)

Antigen Details

Distribution

B cells, Dendritic cells, pDCs

Cell Type
B cells, Dendritic cells
Biology Area
Bacterial proteins and Toxins, Immuno-Oncology, Immunology, Innate Immunity
Molecular Family
CD Molecules, Innate Immune Signaling, Toll Like Receptors
Antigen References
  1. Onji M, et al. 2013. Nat. Commun. DOI: 10.1038/ncomms2949
  2. Krug A, et al. 2001. Eur J Immunol. 10:3026-37.
Gene ID
81897 View all products for this Gene ID
UniProt
View information about CD289 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 07/08/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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