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PE/Cyanine7 anti-mouse CD103 Antibody

Pricing & Availability
Clone
2E7 (See other available formats)
Regulatory Status
RUO
Other Names
Integrin αIEL chain, Integrin αE chain, αE integrin, ITGAE
Isotype
Armenian Hamster IgG
Ave. Rating
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Product Citations
publications
2E7_PECyanine7_CD103_Antibody_1_102623
C57BL/6 mouse splenocytes were stained with CD3 APC and CD103 (clone 2E7) PE/Cyanine7 (left) or Armenian hamster PE/Cyanine7 isotype control (right).
  • 2E7_PECyanine7_CD103_Antibody_1_102623
    C57BL/6 mouse splenocytes were stained with CD3 APC and CD103 (clone 2E7) PE/Cyanine7 (left) or Armenian hamster PE/Cyanine7 isotype control (right).
Compare all formats See PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
121425 25 µg $159
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121426 100 µg $370
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Description

CD103 is a type I transmembrane glycoprotein known as αE integrin or Integrin αIEL chain. It belongs to the integrin family and is primarily found on intestinal intraepithelial lymphocytes (IEL). CD103 is also expressed on a subpopulation of lamina propria T cells, epithelial dendritic cells, lamina propria-derived dendritic cells, and a small subset of peripheral lymphocytes. T regulatory cells express high level of CD103. The CD103 expression on lymphocytes can be induced upon activation and TGF-β stimulation. In association with integrin β7, CD103 is expressed as αE/β7 heterodimer. Mature CD103 protein can be cleaved into 2 chains, a 150 kD (C-terminal) chain and a 25 kD (N-terminal) chain, which remain linked by disulfide bonds. CD103 binds to E-cadherin and mediates homing of lymphocytes to the intestinal epithelium.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse intestinal intraepithelial lymphocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.65 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemical staining1,7 of acetone-fixed frozen sections, immunofluorescence2, and in vitro activation1.

Application References

(PubMed link indicates BioLegend citation)
  1. LeFrancois L, et. al, 1994. Eur. J. Immunol. 24:635. (FC, IHC, IP)
  2. Mysorekar IU, et. al, 2002. J. Biol. Chem. 277:37811. (FC, IF)
  3. Mikami N, et al. 2011. J. Immunol. 186:6886. PubMed
  4. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
  5. Quinn KM, et al. 2013. J. Immunol. 191:5085. PubMed
  6. Verhagen J and Wraith DC. 2014. J. Immunol. Methods. S0022. (FC) PubMed
  7. Xiao B, et al. 2015. PLoS One 1:e0115333. (IHC)
Product Citations
  1. Heindl S, et al. 2021. J Exp Med. 218:. PubMed
  2. Kang H, et al. 2022. Adv Mater. 34:e2106500. PubMed
  3. Seclì L, et al. 2023. J Immunother Cancer. 11:. PubMed
  4. Bhaskar A, et al. 2023. iScience. 26:106644. PubMed
  5. de Mingo Pulido , et al. 2021. Immunity. 54(6):1154-1167.e7. PubMed
  6. Acharya N, et al. 2020. Immunity. 53(3):658-671.e6. PubMed
  7. Chow MT et al. 2019. Immunity. 50(6):1498-1512 . PubMed
  8. Álvaro de Mingo Pulido et al. 2018. Cancer cell. 33(1):60-74 . PubMed
  9. Antunes KH, et al. 2022. Front Immunol. 13:867022. PubMed
  10. Ontiveros-Padilla L, et al. 2021. Microorganisms. 9:. PubMed
  11. Sandborn WJ, et al. 2021. Gastroenterology. 161:1853. PubMed
  12. Lamubol J, et al. 2021. Food Funct. 12:8044. PubMed
  13. Zhou Y, et al. 2022. Nat Commun. 13:2906. PubMed
  14. Len-Letelier RA, et al. 2020. Frontiers in Immunology. 11:583382. PubMed
  15. Hernández–Santos N, et al. 2018. Cell Host Microbe. 1.313194444. PubMed
  16. Reinfeld BI, et al. 2021. Nature. 593:282. PubMed
  17. Milner JJ, et al. 2020. Immunity. 52(5):808-824.e7. PubMed
  18. Niknam S, et al. 2018. Clin Cancer Res. 24:5735. PubMed
  19. Li C, et al. 2020. Immunity. 52(1):201-202. PubMed
  20. Seki T, et al. 2015. J Biochem. 158: 485 - 495. PubMed
  21. Younes AI, et al. 2021. Transl Oncol. 14:100983. PubMed
  22. Guendel F, et al. 2020. Immunity. 53(5):1015-1032.e8. PubMed
  23. Hoover AR, et al. 2022. Clin Transl Med. 12:e937. PubMed
  24. Gardner A, et al. 2022. J Immunother Cancer. 10:. PubMed
  25. Celias DP, et al. 2022. STAR Protoc. 3:101464. PubMed
  26. Chang MH, et al. 2021. Cell Rep. 37:109902. PubMed
RRID
AB_2563690 (BioLegend Cat. No. 121425)
AB_2563690 (BioLegend Cat. No. 121426)

Antigen Details

Structure
Type I transmembrane glycoprotein, Integrin family, can be cleaved into 150 kD and 25 kD chains, associated with β7 integrin
Distribution

Majority of intestinal intraepithelial lymphocytes (IEL), subpopulation of lamina propria T cells, epithelial dendritic cells, small subset of peripheral lymphocytes, Treg cells.

Function
Retention and activation of CD103+ lymphocytes in the intestinal epithelium, regulate tissue-specific T cell homing.
Ligand/Receptor
E-Cadherin
Cell Type
Dendritic cells, Lymphocytes, T cells, Tregs
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Kilshaw PJ and SJ. Murant. 1990. Eur. J. Immunol. 20:2201.
2. Karecla PI, et al. 1995. Eur. J. Immunol. 25:852.
3. LeFrancois L, et al. 1994. Eur. J. Immunol. 24:635.
4. Sung SS, et al. 2006. J. Immunol. 176:2161.
5. Johansson-Lindbom B, et al. 2005. J. Exp. Med. 202:1063.
6. Dujardin HC, et al. 2004. Proc. Natl. Acad. Sci. USA. 101:14473.

Gene ID
16407 View all products for this Gene ID
UniProt
View information about CD103 on UniProt.org

Related FAQs

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Go To Top Version: 3    Revision Date: 04/11/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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