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PE anti-mouse CD317 (BST2, PDCA-1) Antibody

Pricing & Availability
Clone
927 (See other available formats)
Regulatory Status
RUO
Other Names
BST2, tetherin, HM1.2 antigen, bone marrow stromal antigen 2, PDCA-1
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
927_PE_111709
C57BL/6 splenocytes stained with RA3-6B2 (B220) APC and 927 PE
  • 927_PE_111709
    C57BL/6 splenocytes stained with RA3-6B2 (B220) APC and 927 PE
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127009 25 µg $98
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127010 100 µg $335
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Description

CD317, known as BST2, tetherin, HM1.2 antigen, bone marrow stromal antigen 2, or PDCA-1, is type II transmembrane glycoprotein with a molecular mass of 29-33 kD. It is predominantly expressed on Type I IFN-producing cells (IPCs) in naïve mice, but is up-regulated on most cell types following stimulation with type I IFNs and IFN-gamma. It is highly expressed on terminally differentiated normal plasmacytoid dendritic cells and some tumor cells, such as multiple myeloma, renal cell carcinoma, and melanoma cells. BST2 is a recently identified, IFN-induced cellular response factor that blocks release of HIV-1 and other retroviruses from infected cells. BST2 has been found to be the natural ligand of ILT7 in human model.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse plasmacytoid dendritic cells (DCs)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence microscopy, functional assay2, and depletion3,4. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays.

Application References

(PubMed link indicates BioLegend citation)
  1. Blasius AL, et al. 2006. J. Immunol. 177:3260.
  2. Schliemann C, et al. 2010. Blood 115:736. (FA, IF)
  3. Rajagopal D, et al. 2010. Blood 115:1949. (Depletion)
  4. Moniz RJ, et al. 2010. FEMS Immunol. Med. Microbiol. 58:397. (Depletion)
  5. Chen YL, et al. 2013. J Exp Med. 210:2515. PubMed
Product Citations
  1. Gardet A, et al. 2019. Front Immunol. 275:10. PubMed
  2. Chang CA, et al. 2022. Cell Rep. 111615:41. PubMed
  3. Kunimura K, et al. 2020. Cell Reports. 29(9):2823-2834.e7.. PubMed
  4. de Mingo Pulido , et al. 2021. Immunity. 54(6):1154-1167.e7. PubMed
  5. Álvaro de Mingo Pulido et al. 2018. Cancer cell. 33(1):60-74 . PubMed
  6. Kimura T, et al. 2020. Commun Biol. 0.527777778. PubMed
  7. Toivonen R, et al. 2016. J Immunol. 196: 4750 - 4759. PubMed
  8. Uddback I, et al. 2016. Sci Rep. 6:20137. PubMed
  9. Wanner-Seleznik GM, et al. 2020. Immunohorizons. 0.644444444. PubMed
  10. Kubota S, et al. 2019. Nat Commun. 10:1653. PubMed
  11. Zegarra‐Ruiz DF et al. 2018. Cell host & microbe. 25(1):113-127 . PubMed
  12. Lee A, et al. 2022. Nat Commun. 13:549. PubMed
RRID
AB_1953284 (BioLegend Cat. No. 127009)
AB_1953284 (BioLegend Cat. No. 127010)

Antigen Details

Structure
Type II transmembrane glycoprotein with a molecular mass of 29-33 kD.
Distribution

Expressed on type I IFN-producing cells, plasmacytoid dendritic cells, and neoplastic B cells, such as multiple myeloma.

Function
Recently identified antiviral protein that blocks the release of nascent retrovirus or other particles from infected cells.
Cell Type
Dendritic cells
Biology Area
Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Douglas JL. et al. 2009. J Virol. 83(16):7931
2. Cao W et al. 2009. J. Exp. Med. 206(7):1603
3. Neil SJ. et al. 2008. Nature 451:425

Gene ID
69550 View all products for this Gene ID
UniProt
View information about CD317 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 03/04/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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