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PE anti-mouse CD51 Antibody

Pricing & Availability
Clone
RMV-7 (See other available formats)
Regulatory Status
RUO
Other Names
αV integrin, Vitronectin Receptor, Integrin αV chain, ITGAV
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
RMV-7_PE_051311
C57BL/6 mouse bone marrow cells were stained with CD51 (clone RMV-7) PE (filled histogram) or Rat IgG1, κ PE isotype control (open histogram). Data shown was gated on myeloid cell population.
  • RMV-7_PE_051311
    C57BL/6 mouse bone marrow cells were stained with CD51 (clone RMV-7) PE (filled histogram) or Rat IgG1, κ PE isotype control (open histogram). Data shown was gated on myeloid cell population.
  • RMV-7_PE_1_072611
    Con A stimulated C57BL/6 mouse slenocytes were stained with CD3 APC and CD51 (clone RMV-7) PE (above) or Rat IgG1, κ PE isotype control (below).
  • RMV-7_PE_2_072611
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104105 50 µg $98
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104106 200 µg $304
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Description

CD51 is a 140 kD protein, also known as αV integrin, vitronectin receptor, and integrin αV. It is a member of the integrin family, expressed on activated T cells, polymorphonuclear granulocytes, platelets, blastocysts, and osteoclasts. CD51 forms heterodimers by association with integrins β1, β3, β5 or β6; these complexes then act as receptors for multiple extracellular matrix proteins (ECM). The αv integrin heterodimers have varied functions in development, stimulation/activation and homeostasis. The primary ligands for CD51 complexes are fibronectin, fibrinogen, vitronectin, thrombspondin, von Willebrand factor, and CD31. The RMV-7 antibody has been reported to block binding of CD51 to vitronectin, fibronectin, and CD31 in some cell types, as well as blocking LAK cell cytotoxicity.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
BALB/c mouse IL-2-activated killer (LAK) cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, and blocking1-3 of CD51 adhesion and LAK cell cytotoxicity. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 104109 & 104110).

Application References

(PubMed link indicates BioLegend citation)
  1. Takahashi K, et al. 1990. J. Immunol. 145:4371. (IP Block)
  2. Narumiya S, et al. 1994. Int. Immunol. 6:139. (Block)
  3. Piali L, et al. 1995. J. Cell Biol. 130:451. (Block)
  4. Delpino MV, et al. 2012. J. Leukoc Biol 91:285. PubMed.
Product Citations
  1. Zhang M, et al. 2021. Signal Transduct Target Ther. 6:366. PubMed
  2. Pisterzi P, et al. 2023. Hemasphere. 7:e823. PubMed
  3. Ernst MPT, et al. 2023. Hemasphere. 7:e824. PubMed
  4. Barrett N, et al. 2016. Cell Rep. 16: 1039-1054. PubMed
  5. Kedmi R, et al. 2022. Nature. 610:737. PubMed
  6. Kawai H, et al. 2023. JBMR Plus. 7:e10722. PubMed
  7. Debaugnies M, et al. 2023. Nature. 616:168. PubMed
  8. Sasaki L, et al. 2022. Nat Aging. 2:105. PubMed
  9. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  10. Weng L, et al. 2016. Sci Rep. 6:38632. PubMed
  11. Hu X, et al. 2016. Nat Commun. 7:13095. PubMed
  12. Minutti CM, et al. 2019. Immunity. 50:645. PubMed
  13. Leimkühler NB, et al. 2020. Cell Stem Cell. 28:637. PubMed
  14. Kakiuchi K, et al. 2014. Biol Reprod. 90:82. PubMed
  15. Mann M, et al. 2018. Cell Rep. 25:2992. PubMed
  16. Bai Y, et al. 2021. Front Cell Dev Biol. 9:730095. PubMed
  17. Revenco T, et al. 2019. Cell Rep. 29:1458. PubMed
  18. Morimoto A, et al. 2021. Nat Commun. 12:2136. PubMed
  19. Russler-Germain EV, et al. 2021. Elife. 10:. PubMed
  20. Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
  21. Srivastava S, et al. 2019. Cancer Cell. 35:489. PubMed
  22. Lin C, et al. 2022. Nat Commun. 13:6869. PubMed
  23. Kenswil KJG, et al. 2021. Cell Stem Cell. 28(4):653-670.e11. PubMed
  24. Horiguchi H, et al. 2019. Genes Dev. 33:1641. PubMed
  25. Pastushenko I, et al. 2020. Nature. 589:448. PubMed
  26. Delpino M, et al. 2012. J Leukoc Biol. 91:285. PubMed
  27. Krohn-Grimberghe M, et al. 2020. Nat Biomed Eng. 1076:4. PubMed
  28. Nowlan B, et al. 2019. Haematologica. 105:71. PubMed
  29. Chandrakanthan V, et al. 2016. Proc Natl Acad Sci U S A. 113: 2306-2315. PubMed
  30. Gaylo‐Moynihan A et al. 2019. Immunity. 51(2):298-309 . PubMed
  31. Malenica I, et al. 2021. Nat Commun. 12:5209. PubMed
  32. Young K, et al. 2021. Cell Stem Cell. . PubMed
  33. Guarnerio J, et al. 2018. Nat Commun. 9:66. PubMed
RRID
AB_2129493 (BioLegend Cat. No. 104105)
AB_2129493 (BioLegend Cat. No. 104106)

Antigen Details

Structure
Integrin family, associates with integrins β1, β3, β5 or β6, 140 kD
Distribution

Activated T cells, polymorphonuclear granulocytes, blastocysts, osteoclasts

Function
Adhesion
Ligand/Receptor
Fibronectin, fibrinogen, vitronectin, thrombspondin, von Willebrand factor, and CD31
Cell Type
Granulocytes, Mesenchymal Stem Cells, Osteoclasts, T cells
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Maxfield SR, et al. 1989. J. Exp. Med. 169:2173.
3. Piali L, et al. 1995. J. Cell Biol. 130:451.

Gene ID
16410 View all products for this Gene ID
UniProt
View information about CD51 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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