Loading...

PE anti-mouse IL-17A Antibody

Pricing & Availability
Clone
TC11-18H10.1 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-17, Cytotoxic T lymphocyte-associated antigen 8 (CTLA-8)
Isotype
Rat IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
TC11-18H10.1_PE_041910
PMA/ionomycin-stimulated (5 hours) Th17 polarized C57BL/6 mouse CD4+ T cells surface stained with CD4 (GK1.5) APC, then intracellularly stained with TC11-18H10.1 PE
  • TC11-18H10.1_PE_041910
    PMA/ionomycin-stimulated (5 hours) Th17 polarized C57BL/6 mouse CD4+ T cells surface stained with CD4 (GK1.5) APC, then intracellularly stained with TC11-18H10.1 PE
  • TC11-18H10_PE_060806
    PMA (20 ng/ml) + ionomycin (1 µg/ml) -stimulated (6 hours + monensin, 2 µM) mouse thymoma cell line EL-4 intracellularly stained with TC11-18H10.1 PE
Compare all formats See PE spectral data
Cat # Size Price Quantity Check Availability Save
506903 25 µg $110
Check Availability


Need larger quantities of this item?
Request Bulk Quote
506904 100 µg $280
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

IL-17, also known as CTLA-8, is a T cell-expressed pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpes virus Saimiri. IL-17 is produced by Th cells (Th17) that are distinct from the traditional Th1- and Th2-cell subsets. IL-23 plays an important role in triggering IL-17 production. Both recombinant and natural IL-17 have been shown to exist as disulfide linked homodimers. IL-17 exhibits multiple biological activities on a variety of cells including: the induction of IL-6 and IL-8 production in fibroblasts, activation of NF-κB, and costimulation of T cell proliferation. IL-17 is an essential inflammatory mediator in the development of autoimmune diseases. Neutralization of IL-17 with monoclonal antibody is able to ameliorate the disease course.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
E. coli expressed, recombinant mouse IL-17A
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The IL-17A antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

ELISA Capture3,4 and ELISPOT Capture5: The purified TC11-18H10.1 antibody is useful as the capture antibody in a sandwich ELISA, when used in conjunction with the biotinylated TC11-8H4 antibody (Cat. No. 507002) as the detecting antibody and recombinant mouse IL-17 (Cat. No. 576009) as the standard.
Flow Cytometry2-4,7,8,11,12: The TC11-18H10.1 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-17-producing cells within mixed cell populations.
Neutralization6,9: The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse IL-17 bioactivity in vivo and in vitro (Cat. No. 506906).

Application References

(PubMed link indicates BioLegend citation)
  1. Kennedy J, et al. 1996. J. Interferon Cytokine Res. 16:611.
  2. Schubert D, et al. 2004. J. Immunol. 172:4503. (ICFC)
  3. Infante-Duarte C, et al. 2000. J. Immunol. 165:6107. (ICFC, ELISA Capture)
  4. Harrington LE, et al. 2005. Nature Immunol. doi:10.1038/ni1254. (ICFC, ELISA Capture)
  5. Nekrasova T, et al. 2005. J. Immunol. 175:2734. (ELISPOT Capture)
  6. Yen D, et al. 2006. J. Clin. Invest. 116:1310. (Neut)
  7. Ehirchiou D, et al. 2007. J. Exp. Med. 204:1519. (ICFC)
  8. Kang SG, et al. 2007. J. Immunol. 179:3724. (ICFC)
  9. Smith E, et al. 2008. J. Immunol. 181:1357. (Neut) PubMed
  10. Neufert C, et al. 2007. Eur. J. Immunol. 37:1809. PubMed
  11. Wang C, et al. 2009. Mucosal Immunol 2:173. (ICFC) PubMed
  12. Cui Y, et al. 2009. Invest. Ophth. Vis. Sci. 50:5811. (ICFC) PubMed
  13. Kivisäkk P, et al. 2009. Ann. Neurol. 65:457. PubMed
  14. Cooney LA, et al. 2011. J. Immunol. 187:4440. PubMed
  15. Ma Y, et al. 2012. PLoS One. 7:e40763. PubMed
  16. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
Product Citations
  1. Wu J et al. 2017. Immunity. 47(6):1114-1128 . PubMed
  2. Laura C Burzynski et al. 2019. Immunity. 50(4):1033-1042 . PubMed
  3. Kimura S, et al. 2020. Nat Commun. 0.620833333. PubMed
  4. Vono M, et al. 2020. Cell Reports. 28(7):1773-1784.e5.. PubMed
  5. Xue F, et al. 2020. Int J Biol Sci. 2.105555556. PubMed
  6. Garo LP, et al. 2021. Nat Commun. 12:2419. PubMed
  7. Tian J, et al. 2021. Autophagy. 17:2586. PubMed
  8. Zheng J, et al. 2022. Front Pharmacol. 13:866228. PubMed
  9. Yamazaki S, et al. 2022. Mucosal Immunol. :. PubMed
  10. Tian WJ, et al. 2021. Clin Exp Pharmacol Physiol. 48:902. PubMed
  11. Yu C, et al. 2022. Invest Ophthalmol Vis Sci. 63:1. PubMed
  12. Hong Y, et al. 2022. J Leukoc Biol. 112:425. PubMed
  13. Chen C, et al. 2022. Sci Adv. 8:eabo4363. PubMed
  14. Mansouri S, et al. 2022. J Immunol. 209:2114. PubMed
  15. Ge Y, et al. 2022. iScience. 25:105437. PubMed
  16. Dölz M, et al. 2022. iScience. 25:105372. PubMed
  17. Zhu M, et al. 2022. Front Immunol. 13:1019248. PubMed
  18. Xu H, et al. 2022. Aging Dis. 13:1875. PubMed
  19. Weng S, et al. 2022. Front Immunol. 13:1025931. PubMed
  20. Zhao Q, et al. 2023. Nutrients. 15: . PubMed
  21. Zhang B, et al. 2023. Signal Transduct Target Ther. 8:28. PubMed
  22. Xu R 2023. Cell Reports. 42(2):112054. PubMed
  23. Wight AE, et al. 2022. Proc Natl Acad Sci U S A. 119:e2200757119. PubMed
  24. Zhao L, et al. 2022. Oncogene. 41:4200. PubMed
  25. Deák P, et al. 2022. Cell Rep. 41:111563. PubMed
  26. Wilson NG, et al. 2023. iScience. 26:105991. PubMed
  27. Aso K, et al. 2023. Nat Commun. 14:984. PubMed
  28. Li G, et al. 2023. Cell Death Dis. 14:185. PubMed
  29. Schwarz A, et al. 2023. Front Immunol. 14:1038689. PubMed
  30. Giampaolo S, et al. 2023. iScience. 26:106234. PubMed
  31. Mabire M, et al. 2023. Nat Commun. 14:1830. PubMed
  32. Yan YN, et al. 2023. Mediators Inflamm. 2023:8215567. PubMed
  33. Cai X, et al. 2023. Nat Commun. 14:2004. PubMed
  34. Levra Levron C, et al. 2023. Nat Cell Biol. 25:740. PubMed
  35. Files M, et al. 2022. NPJ Vaccines. 7:48. PubMed
  36. Jong RM, et al. 2022. J Immunol. 208:407. PubMed
  37. Qian Y, et al. 2021. Cell Reports. 36(8):109602. PubMed
  38. Yang R, et al. 2020. Cell Death Dis. 0.877083333. PubMed
  39. Du C, et al. 2016. Nat Commun. 7: 11120. PubMed
  40. Kang S, et al. 2007. J Immunol. 179:3724. PubMed
  41. Yang C, et al. 2018. J Immunol. 200:1316. PubMed
  42. Saleh MM, et al. 2019. Cell Host Microbe. 25:756. PubMed
  43. Kenichi Shimada et al. 2018. Immunity. 49(5):873-885 . PubMed
  44. Liu QZ, et al. 2018. Front Immunol. 1.131944444. PubMed
  45. Angiari S, et al. 2020. Cell Metab. 31:391. PubMed
  46. Su N, Xiong Y 2016. Sci Rep. 6:22658. PubMed
  47. Pan Y, et al. 2021. Front Cell Neurosci. 15:664312. PubMed
  48. Ni J, et al. 2022. Mol Med. 28:65. PubMed
  49. Zhao Y, et al. 2015. PLoS One. 10: 0134797. PubMed
  50. Piper CJM, et al. 2020. Cell Reports. 29(7):1878-1892.e7.. PubMed
  51. Lu Y, et al. 2019. Int J Mol Med. 43:2398. PubMed
  52. Ogawa C et al. 2018. Cell reports. 25(1):19-28 . PubMed
  53. Han Y et al. 2019. Cell reports. 27(3):835-846 . PubMed
  54. Kuwahara M, et al. 2014. Nat Commun. 5:3555. PubMed
  55. Nagaoka M, et al. 2014. J Immunol. 193:2812. PubMed
  56. Yasuoka T, et al. 2016. PLoS One. 11: 0157395. PubMed
  57. Wagner V, et al. 2016. Sci Transl Med. 8: 366ra164. PubMed
  58. D Kim, S Lee, H Jun 2016. Immunol Cell Biol. 10.1038/icb.2016.98. PubMed
  59. Okuniewska M, et al. 2021. Cell Reports. 36(2):109368. PubMed
  60. Aqel SI, et al. 2021. JCI Insight. 6:e142376. PubMed
  61. Zhou HF, et al. 2022. Front Pharmacol. 13:778755. PubMed
  62. Sehnert B, et al. 2022. Front Immunol. 13:921488. PubMed
  63. Alissafi T, et al. 2020. Cell Metabolism. 32(4):591-604.e7. PubMed
  64. Liu YJ, et al. 2020. Theranostics. 10:5225. PubMed
  65. Britton GJ et al. 2019. Immunity. 50(1):212-224 . PubMed
  66. He J, et al. 2020. Cell Reports. 29(9):2718-2730.e6.. PubMed
  67. Zhao F, et al. 2022. Front Immunol. 13:873720. PubMed
  68. Pu Y, et al. 2022. Transl Psychiatry. 12:223. PubMed
  69. Mansouri S, et al. 2021. J Immunol. 206:2233. PubMed
  70. Zhang B, et al. 2021. Nat Biomed Eng. 5:1288. PubMed
  71. Kawakami R, et al. 2021. Immunity. 54(5):947-961.e8. PubMed
  72. Ni P, et al. 2014. Mol Immunol. 62:199. PubMed
  73. Niwa H, et al. 2009. Clin Immunol. 132:184. PubMed
  74. Luo Y et al. 2019. Cell reports. 26(7):1869-1879 . PubMed
  75. Colliou N, et al. 2018. Gut Microbes. 9:279. PubMed
  76. Dar HY, et al. 2018. Sci Rep. 8:2503. PubMed
  77. Dar HY, et al. 2018. Bone Rep. 8:46. PubMed
  78. Bhattacharya D, et al. 2014. J Biol Chem. 289:16508. PubMed
  79. Wang J, et al. 2021. Nat Commun. 12:6198. PubMed
  80. Mara AB, et al. 2022. NPJ Vaccines. 7:86. PubMed
  81. Yong L, et al. 2022. Nat Commun. 13:4255. PubMed
  82. Zeng Q, et al. 2022. Front Immunol. 13:740805. PubMed
  83. Takeshita Y, et al. 2021. Neurol Neuroimmunol Neuroinflamm. 8:. PubMed
  84. Ouyang W, et al. 2021. Invest Ophthalmol Vis Sci. 62:25:00. PubMed
  85. Bankoti R, et al. 2017. Sci Rep. 10.1038/s41598-017-12171-3. PubMed
  86. Castro–Dopico T, et al. 2020. Cell Reports. 32(1):107857. PubMed
  87. Kawashima H, et al. 2013. J Immunol. 191:3614. PubMed
  88. Luck H, et al. 2015. Cell Metab. 21 527 . PubMed
  89. Deerhake ME, et al. 2021. Immunity. 54(3):484-498.e8. PubMed
  90. Vaena S, et al. 2021. Cell Reports. 35(5):109076. PubMed
  91. Alhosaini K, et al. 2021. Brain Sci. :11. PubMed
  92. Tan YY, et al. 2021. Exp Ther Med. 22:1368. PubMed
  93. Wen M, et al. 2022. JCI Insight. Online ahead of print. PubMed
  94. Lin YW, et al. 2022. Mol Med Rep. 26:. PubMed
  95. Chou T, et al. 2016. Nat Commun. 7:11904. PubMed
  96. Takeuchi H, et al. 2013. J Immunol. 191:3725. PubMed
  97. Park S, et al. 2011. J Immunol. 187:5813. PubMed
  98. Chatterjee S et al. 2017. Cell metabolism. 27(1):85-100 . PubMed
  99. Van Dis E, et al. 2018. Cell Rep. 23:1435. PubMed
  100. Ramakrishnan SK, et al. 2019. Nat Commun. 10:660. PubMed
  101. Katagiri T, et al. 2019. Mucosal Immunol. 12:p1104. PubMed
  102. Xiao S, et al. 2020. Cell Reports. 32(2):107892. PubMed
  103. Zeng Q, et al. 2022. iScience. 25:105151. PubMed
  104. Ghorbani S, et al. 2022. Nat Commun. 13:2445. PubMed
  105. Derada Troletti C, et al. 2021. Cell Reports. 35(9):109201. PubMed
  106. Wang G, et al. 2021. Cell Host Microbe. 29(5):777-791.e6. PubMed
  107. Wu B, et al. 2021. Immunity. 54(2):308-323.e6. PubMed
  108. Mansouri S, et al. 2020. Mucosal Immunol. 0.954861111. PubMed
  109. Kang J, Lee J, Chang J 2016. PLoS One. 11: 0157015. PubMed
  110. Gorman JA, et al. 2019. Front Immunol. 10:44. PubMed
  111. Ouyang S, et al. 2019. J Immunol. 202:1441. PubMed
  112. Sanchez M et al. 2017. Cell host & microbe. 22(4):543-551 . PubMed
  113. Luck H, et al. 2019. Nat Commun. 10:3650. PubMed
  114. Metidji A et al. 2018. Immunity. 49(2):353-362 . PubMed
  115. Kang YH, et al. 2019. Nat Commun. 10:912. PubMed
  116. Ehirchiou D, et al. 2007. J Exp Med . 204:1519. PubMed
  117. Li X, et al. 2016. MBio. 7: 02232-15. PubMed
  118. Miura N, et al. 2017. Mol Ther. 10.1016/j.ymthe.2017.01.020. PubMed
  119. Lai Y, et al. 2022. Clin Transl Med. 12:e999. PubMed
  120. Karanika S, et al. 2022. Front Immunol. 13:972266. PubMed
  121. Zhang Y, et al. 2021. Commun Biol. 344:4. PubMed
  122. Mizutani N, et al. 2012. J Immunol. 188:5694. PubMed
  123. Suhail A, et al. 2019. Cell Rep. 29:3522. PubMed
  124. Tian D, et al. 2019. Nat Commun. 10:4246. PubMed
  125. Castro‐Dopico T et al. 2019. Immunity. 50(4):1099-1114 . PubMed
  126. Zegarra‐Ruiz DF et al. 2018. Cell host & microbe. 25(1):113-127 . PubMed
  127. Jeon Y, et al. 2015. PLoS One. 10: e0139845. PubMed
  128. Hao L, et al. 2021. Br J Pharmacol. 178:4726. PubMed
  129. Yue T, et al. 2022. Cell Death Differ. 29:218. PubMed
  130. Huang LJ, et al. 2021. Immunity. 54(8):1728-1744.e7. PubMed
  131. Yu H, et al. 2015. PLoS One. 10: 0143001. PubMed
  132. Charlton J, et al. 2015. PLoS One. 10:119200. PubMed
  133. Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed
  134. Lai NY, et al. 2020. Cell. 180:33:00. PubMed
  135. Wang N, et al. 2020. Front Immunol. 1.765972222. PubMed
  136. Li J, et al. 2012. Arthritis Rheum. 64:1098. PubMed
  137. Li C, et al. 2014. J Immunol. 192:1425. PubMed
  138. Jaeger N, et al. 2020. Cell Rep. 33:108331. PubMed
  139. Toriyama K, et al. 2020. Commun Biol. 0.398611111. PubMed
  140. Wang J, et al. 2020. Cell. 183(7):1867-1883.e26. PubMed
  141. Tang L, et al. 2022. Front Immunol. 12:770402. PubMed
  142. Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed
  143. Mansouri S, et al. 2021. J Immunol. 206:2233. PubMed
  144. Aggarwal N, et al. 2021. Cell Rep. 37:110170. PubMed
  145. Dai B, et al. 2021. Cell Reports Medicine. 2(8):100381. PubMed
  146. Pahari S, et al. 2020. Autophagy. 1.375694444. PubMed
  147. Ge Y, et al. 2020. Mucosal Immunol. 13:34. PubMed
  148. Bing Wu et al. 2018. Immunity. 49(5):886-898 . PubMed
RRID
AB_315463 (BioLegend Cat. No. 506903)
AB_315463 (BioLegend Cat. No. 506904)

Antigen Details

Structure
Cytokine; dimer; 15 kD (Mammalian).
Bioactivity
Secretion of IL-6, IL-8, G-CSF, prostaglandin E2 by epithelial, endothelial or fibroblastic cells; stimulates cell migration, cord formation, and IL-6 secretion by stromal cells
Cell Sources
CD4+ memory T cells
Cell Targets
Fibroblasts, epithelial and endothelial cells, stromal cells
Receptors
IL-17R (CD217)
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Numasaki M, et al. 2002. Blood 101:2620.
3. Fossiez F, et al. 1996. J. Exp. Med. 183:2593.
4. Yao Z, et al. 1997. Cytokine 9:794.
5. Dong C. 2006. Nat. Rev. Immunol. 6:329.
6. Hofstetter HH, et al. 2005 Cell. Immunol. 237:123.

Gene ID
16171 View all products for this Gene ID
UniProt
View information about IL-17A on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Does the anti-mouse IL-17 antibody (clone TC11-18H10.1) recognize the A or F isoform?
Clone TC11-18H10.1 recognizes IL-17A isoform, but it also recognizes the IL-17A/F heterodimer via IL-17A binding.

Other Formats

View All IL-17A Reagents Request Custom Conjugation
Description Clone Applications
PE anti-mouse IL-17A TC11-18H10.1 ICFC
Purified anti-mouse IL-17A TC11-18H10.1 ELISA Capture,CyTOF®,ELISPOT Capture,ICFC
FITC anti-mouse IL-17A TC11-18H10.1 ICFC
Alexa Fluor® 488 anti-mouse IL-17A TC11-18H10.1 ICFC
Alexa Fluor® 647 anti-mouse IL-17A TC11-18H10.1 ICFC
Alexa Fluor® 700 anti-mouse IL-17A TC11-18H10.1 ICFC
APC anti-mouse IL-17A TC11-18H10.1 ICFC
Pacific Blue™ anti-mouse IL-17A TC11-18H10.1 ICFC
PerCP/Cyanine5.5 anti-mouse IL-17A TC11-18H10.1 ICFC
PE/Cyanine7 anti-mouse IL-17A TC11-18H10.1 ICFC
Brilliant Violet 421™ anti-mouse IL-17A TC11-18H10.1 ICFC
Brilliant Violet 605™ anti-mouse IL-17A TC11-18H10.1 ICFC
Brilliant Violet 650™ anti-mouse IL-17A TC11-18H10.1 ICFC
Brilliant Violet 785™ anti-mouse IL-17A TC11-18H10.1 ICFC
Brilliant Violet 510™ anti-mouse IL-17A TC11-18H10.1 ICFC
Purified anti-mouse IL-17A (Maxpar® Ready) TC11-18H10.1 ELISA Capture,CyTOF®
PE/Dazzle™ 594 anti-mouse IL-17A TC11-18H10.1 ICFC
APC/Cyanine7 anti-mouse IL-17A TC11-18H10.1 ICFC
Brilliant Violet 711™ anti-mouse IL-17A TC11-18H10.1 ICFC
PerCP anti-mouse IL-17A TC11-18H10.1 ICFC
Ultra-LEAF™ Purified anti-mouse IL-17A TC11-18H10.1 ELISA Capture,CyTOF®,ELISPOT Capture,Neut,ICFC
PE/Cyanine5 anti-mouse IL-17A TC11-18H10.1 ICFC
Go To Top Version: 2    Revision Date: 10/16/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login/Register
Remember me
Forgot your password? Reset Password
Request an Account