PE/Cyanine7 anti-human CD18 Antibody anti-CD18

Pricing & Availability

Clone: CBR LFA-1/2 (See other available formats)
Regulatory Status: RUO
Other Names: Integrin β2 subunit, LFA -1 β subunit, β2 integrin, ITGB2, b2 integrin, beta 2 integrin
Isotype: Mouse IgG1, κ
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Product Citations: publications

CBRLFA-12_PECy7_CD18_Antibody_121517 — Human peripheral blood lymphocytes were stained with CD18 (clone CBR LFA-1/2) PE/Cyanine7 (filled histogram) or mouse IgG1, κ PE/Cyanine7 isotype control (open histogram).

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366309

25 tests

$171

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366310

100 tests

$358

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Description

CD18, also known as integrin β2 subunit, LFA-1 β subunit, and β2 integrin, is a 90 - 95 kD type I glycoprotein. CD18 non-covalently associates with CD11a, CD11b, or CD11c. CD18 is expressed on all leukocytes. CD18 and associated α chains function in the adhesion and signaling in hematopoietic cells.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Purified human LFA-1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats of this clone) include: activation of LFA-1 and MAC-1^1,2. The LEAF™ or Ultra-LEAF™ purified antibody (Endotoxin < EU/µg, Azide-Free, 0.2 µm filtered) is recommended for function assays (contact our custom solutions team).
RRID: AB_2721657 (BioLegend Cat. No. 366309)
AB_2721657 (BioLegend Cat. No. 366310)

Antigen Details

Structure: 90 - 95 kD type I transmembrane glycoprotein.
Distribution: Leukocytes.
Function: Cell adhesion and signaling.
Interaction: Integrin, type I transmembrane glycoprotein, associates with CD11a, CD11b, or CD11c
Ligand/Receptor: ICAMs.
Cell Type: Leukocytes
Biology Area: Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Anderson DC, et al. 1987. Annu. Rev. Med. 38:175.
2. Springer TA. 1994. Cell 76:301.
Gene ID: 3689 View all products for this Gene ID
UniProt: View information about CD18 on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Pathways

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Other Formats

View All CD18 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human CD18	CBR LFA-1/2	FC
PE anti-human CD18	CBR LFA-1/2	FC
APC anti-human CD18	CBR LFA-1/2	FC
FITC anti-human CD18	CBR LFA-1/2	FC
PE/Cyanine7 anti-human CD18	CBR LFA-1/2	FC
Alexa Fluor® 647 anti-human CD18	CBR LFA-1/2	FC
APC/Fire™ 750 anti-human CD18	CBR LFA-1/2	FC
PerCP/Cyanine5.5 anti-human CD18	CBR LFA-1/2	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 12/18/2017

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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