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PE/Cyanine7 anti-mouse CD49a Antibody

Pricing & Availability
Clone
HMα1 (See other available formats)
Regulatory Status
RUO
Other Names
Very late antigen 1 (VLA1), integrin alpha 1 (ITGA1)
Isotype
Armenian Hamster IgG
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Product Citations
publications
HMa1_PECy7_CD49a_Antibody_062118
C1300 (mouse neuroblastoma cell line) cells were stained with CD49a (clone HMa1) PE/Cyanine7 (filled histogram) or Armenian hamster IgG PE/Cyanine7 isotype control (open histogram).
  • HMa1_PECy7_CD49a_Antibody_062118
    C1300 (mouse neuroblastoma cell line) cells were stained with CD49a (clone HMa1) PE/Cyanine7 (filled histogram) or Armenian hamster IgG PE/Cyanine7 isotype control (open histogram).
Compare all formats See PE/Cyanine7 spectral data
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142607 25 µg $112
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142608 100 µg $335
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Description

CD49a is a 1179 aa, type I transmembrane glycoprotein also known as α1 integrin, VLA-1 α chain, or integrin α1. It associates antibody v042010 with CD29 (β1 integrin) to form the VLA-1 complex, a collagen IV and alminin-1 receptor that is expressed on activated T cells, smooth muscle cells, endothelial cells, neuronal cells, fibroblasts, and mesenchymal cells. CD49a is an adhesion molecule and is involved in the regulation of leukocyte migration, T cell proliferation, and cytokine production.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse Neuroblastoma Cell Line C1300
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant format) include: inhibition of cell adhesion and cytokine production1,2.

Application References

(PubMed link indicates BioLegend citation)
  1. Miyake S, et al. 1994. Eur. J. Immunol. 24:2000. (FC, Block)
  2. Tanaka T, et al. 1995. Int. Immunol. 7:1183. (Block)
Product Citations
  1. Aghayev T, et al. 2022. Cancer Discov. 12:1960. PubMed
RRID
AB_2749930 (BioLegend Cat. No. 142607)
AB_2749930 (BioLegend Cat. No. 142608)

Antigen Details

Structure
1179 aa, type I transmembrane glycoprotein
Distribution

Endothelial cells, fibroblasts, activated T cells

Function
Leukocyte adhesion and homing; costimulation
Ligand/Receptor
Collagens, laminin
Cell Type
Endothelial cells, Fibroblasts, Mesenchymal Stem Cells, T cells
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Innate Immunity, Signal Transduction, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barczyk M, et al. 2010. Cell Tissue Res. 339:269.

Gene ID
109700 View all products for this Gene ID
UniProt
View information about CD49a on UniProt.org
Go To Top Version: 1    Revision Date: 06/25/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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