- Clone
- 2D3 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- CD275, ICOSL, B7H2, ICOS-L, B7RP-1, B7-RP1
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
309423 | 25 tests | $134 | ||||
309424 | 100 tests | $336 |
B7-H2, a member of the B7 family and the immunoglobulin superfamily, is a 40 kD protein also known as B7RP-1, B7h, B7-H2, GL50 and ICOS Ligand. Human B7-H2 is expressed by B lymphocytes, activated monocytes/macrophages, and dendritic cells. B7-H2 binds to a CD28-like receptor, inducible costimulator molecule (ICOS, AILIM, CRP-1), which is expressed by activated T cells. The interaction of ICOS with B7-H2 plays an important role in the T cell costimulation pathway.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human B7H2-mIg fusion protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm. - Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
-
Application References
(PubMed link indicates BioLegend citation) -
- Kurosawa S, et al. 2003. Am. J. Respir. Cell Mol. Biol. 28:563.
- RRID
-
AB_2936696 (BioLegend Cat. No. 309423)
AB_2936696 (BioLegend Cat. No. 309424)
Antigen Details
- Structure
- Ig superfamily, B7 family, 40 kD
- Distribution
-
B cells and dendritic cells
- Function
- Binds ICOS on antigen-activated T cells and mediates proliferation and cytokine production
- Ligand/Receptor
- ICOS
- Cell Type
- B cells, Dendritic cells
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
- Wang S, et al. 2002. J. Biol. Chem. 96:2808.
- Wong SC, et al. 2003. Blood 102:1831.
- Gene ID
- 23308 View all products for this Gene ID
- UniProt
- View information about CD275 on UniProt.org
Other Formats
View All CD275 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
PE anti-human CD275 (B7-H2, ICOSL) | 2D3 | FC |
Purified anti-human CD275 (B7-H2, ICOSL) | 2D3 | FC,IP,ELISA |
Biotin anti-human CD275 (B7-H2, ICOSL) | 2D3 | FC |
APC anti-human CD275 (B7-H2, ICOSL) | 2D3 | FC |
PE/Cyanine7 anti-human CD275 (B7-H2, ICOSL) | 2D3 | FC |
TotalSeq™-A0009 anti-human CD275 (B7-H2, ICOSL) | 2D3 | PG |
Alexa Fluor® 647 anti-human CD275 (B7-H2, ICOSL) | 2D3 | FC |
PerCP/Cyanine5.5 anti-human CD275 (B7-H2, ICOSL) | 2D3 | FC |
TotalSeq™-C0009 anti-human CD275 (B7-H2, ICOSL) | 2D3 | PG |
TotalSeq™-B0009 anti-human CD275 (B7-H2, ICOSL) | 2D3 | PG |
PE/Dazzle™ 594 anti-human CD275 (B7-H2, ICOSL) | 2D3 | FC |
TotalSeq™-D0009 anti-human CD275 (B7-H2, ICOSL) | 2D3 | PG |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
PE anti-human CD275 (B7-H2, ICOSL)
Human monocyte-derived dendritic cells stained with 2D3 PE -
Purified anti-human CD275 (B7-H2, ICOSL)
Human monocyte-derived dendritic cells stained with purified... -
Biotin anti-human CD275 (B7-H2, ICOSL)
Human monocyte-derived dendritic cells stained with biotinyl... -
APC anti-human CD275 (B7-H2, ICOSL)
Human Burkitt's lymphoma cell line Daudi was stained with CD... -
PE/Cyanine7 anti-human CD275 (B7-H2, ICOSL)
Human Burkitt’s lymphoma cell line, Daudi was stained with... -
TotalSeq™-A0009 anti-human CD275 (B7-H2, ICOSL)
-
Alexa Fluor® 647 anti-human CD275 (B7-H2, ICOSL)
Human Burkitt's™ lymphoma cell line, Daudi was stained with ... -
PerCP/Cyanine5.5 anti-human CD275 (B7-H2, ICOSL)
Peripheral blood mononuclear cells stimulated with Recombina... -
TotalSeq™-C0009 anti-human CD275 (B7-H2, ICOSL)
-
TotalSeq™-B0009 anti-human CD275 (B7-H2, ICOSL)
-
PE/Dazzle™ 594 anti-human CD275 (B7-H2, ICOSL)
Peripheral blood mononuclear cells stimulated with recombina... -
TotalSeq™-D0009 anti-human CD275 (B7-H2, ICOSL)
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